A5011536713- Vector-borne infectious diseases
- Estrogen and related hormone effects
- Viral Infections and Vectors
- Neuroscience and Neuropharmacology Research
- Receptor Mechanisms and Signaling
- Veterinary medicine and infectious diseases
- Helicobacter pylori-related gastroenterology studies
- Vector-Borne Animal Diseases
- Yersinia bacterium, plague, ectoparasites research
- Stress Responses and Cortisol
- Neuropeptides and Animal Physiology
- Pain Mechanisms and Treatments
- Galectins and Cancer Biology
- Protein Kinase Regulation and GTPase Signaling
- RNA and protein synthesis mechanisms
- Antimicrobial Peptides and Activities
- Molecular Biology Techniques and Applications
- Pharmacogenetics and Drug Metabolism
- Medical Imaging Techniques and Applications
- Signaling Pathways in Disease
- Bacteriophages and microbial interactions
- Immune Response and Inflammation
- Animal Ecology and Behavior Studies
- Neurotransmitter Receptor Influence on Behavior
- Mosquito-borne diseases and control
Université de Poitiers
2008-2020
Institut Pasteur
2006-2017
Centre Hospitalier Universitaire de Poitiers
2005-2016
GlaxoSmithKline (Italy)
2002-2014
Laboratoire National de Référence
2010
GlaxoSmithKline (United Kingdom)
2010
Assistance Publique Hôpitaux de Marseille
2009
International Centre for Diarrhoeal Disease Research
2005
DuPont (United States)
2003
Inserm
1987-2003
ABSTRACT Analysis of Lyme borreliosis (LB) spirochetes, using a novel multilocus sequence analysis scheme, revealed that OspA serotype 4 strains (a rodent-associated ecotype) Borrelia garinii were sufficiently genetically distinct from bird-associated B. to deserve species status. We suggest be raised status and named bavariensis sp. nov. The rooted phylogenetic trees provide insights into the evolutionary history LB spirochetes.
To evaluate the role of mitochondrial peripheral-type benzodiazepine receptor (PBR) in steroidogenesis, we developed a molecular approach based on disruption PBR gene, by homologous recombination, constitutive steroid producing R2C rat Leydig tumor cell line. Inactivation one allele gene resulted suppression mRNA and ligand binding expression. Immunoblot electron microscopic immunogold labeling analyses confirmed absence 18-kDa protein selected clone. Although mitochondria from PBR-negative...
While the spread of Salmonella enterica serotype Kentucky resistant to ciprofloxacin across Africa and Middle-East has been described recently, presence this strain in humans, food, various animal species (livestock, pets, wildlife) environment is suspected other countries different continents. Here, we report results an in-depth molecular epidemiological study on a global human non-human collection S. (n = 70). We performed XbaI-pulsed field gel electrophoresis multilocus sequence typing,...
Gonadal steroidogenesis is regulated by pituitary gonadotropins and a locally produced, unidentified factor. A 70-kilodalton (kD) protein complex secreted from rat Sertoli cells was isolated. The complex, composed of 28- 38-kD proteins, stimulated Leydig ovarian granulosa in dose-dependent adenosine 3′,5′-monophosphate-independent manner. follicle-stimulating hormone-induced 28-kD appeared to be responsible for the bioactivity, but indispensable maximal activity. proteins were shown...
The peripheral-type benzodiazepine receptor (PBR) was identified and characterized by its high affinity for two distinct classes of compounds, the benzodiazepines (BZs) isoquinolines (IQs). An M(r) 18,000 IQ-binding protein has been as PBR. In this report we isolated sequenced a 626-base pair cDNA, specifying an open reading frame 169 amino acid residues with predicted molecular weight 18,843, from MA-10 mouse tumor Leydig cells [i.e., (mPBR)]. Expression mPBR cDNA in simian virus...
(-)-cis-1-Methyl-7-[[4-(2,6-dichlorophenyl)piperidin-1-yl]methyl]-6,7,8,9-tetrahydro-5H-benzocyclohepten-5-ol (SB-612111) is a novel human opiate receptor-like orphan receptor (ORL-1) antagonist that has high affinity for the clonal ORL-1 (hORL-1 K(i) = 0.33 nM), selectivity versus mu-(174-fold), delta-(6391-fold), and kappa (486-fold)-opioid receptors able to inhibit nociceptin signaling via hORL-1 in whole cell gene reporter assay. SB-612111 no measurable antinociceptive effects vivo mouse...
Previous studies demonstrated that the polypeptide diazepam binding inhibitor (DBI) and its receptor, peripheral-type benzodiazepine receptor (PBR), are involved in regulation of steroid biosynthesis one site PBR action resides mitochondria. In present investigation, evidence is presented a functional form also at cell surface. First, was immunolocalized rat testis using biotin-streptavidin peroxidase immunocytochemistry, results revealed exclusively interstitial Leydig cells. Next,...
The specific involvement of the delta-opioid receptor in control nociception was explored by investigating pharmacological activity vivo a selective, orally active, and centrally penetrant agonist. [8R-(4bS*,8aalpha,8abeta,12bbeta)]7,10-dimethyl-1-methoxy-11-(2-methylpropyl)oxycarbonyl 5,6,7,8,12,12b-hexahydro-(9H)-4,8-methanobenzofuro[3,2-e]pyrrolo[2,3-g]isoquinoline hydrochloride (SB-235863) is new pyrrolomorphinan with high affinity (Ki = 4.81 +/- 0.39 nM) for receptor, full agonist...
The polypeptide diazepam binding inhibitor (DBI) and drug ligands for the mitochondrial peripheral-type benzodiazepine receptor (PBR) have been shown to regulate cholesterol transport, rate-determining step in steroidogenesis, hormone-responsive steroidogenic cells including MA-10 Leydig tumor cells. present study was designed characterize role of DBI PBR R2C rat constitutive steroid-producing cell model. Both were treatment with a cholesterol-linked phosphorothioate oligodeoxynucleotide...
Due to the high Lyme borreliosis incidence in Alsace, northeastern France, we investigated 2003-2004 three cantons this region order determine density of Ixodes ricinus ticks infected by Borrelia burgdorferi sensu lato and Anaplasmataceae. The peak nymphs B. at Munster Guebwiller, where disease was high, among highest reported Europe (105 114 per 100 m(2), respectively). In contrast, low canton Dannemarie (5/100 m(2)), low. two main species detected were afzelii, more frequent nymphs,...
ABSTRACT We developed a single-vessel multiplex real-time PCR assay that detects Helicobacter pylori infection and identified the four existing alleles of 23S rRNA genes H. —the wild-type sequence three mutations conferring clarithromycin resistance—using allele-specific Scorpion primers directly on biopsy specimens. The combine primer probe in single molecule are able to distinguish single-nucleotide polymorphism. Fluorescent signals, produced when probes annealed, read channels by...
Background Leptospirosis has long been a major public health concern in the southwestern Indian Ocean. However, Madagascar, only few, old studies have provided indirect serological evidence of disease humans or animals. Methodology/Principal Findings We conducted large animal study focusing on small-mammal populations. Five field trapping surveys were carried out at five sites, from April 2008 to August 2009. Captures consisted Rattus norvegicus (35.8%), R. rattus (35.1%), Mus musculus...
Borrelia burgdorferi sl is a complex of pathogen bacteria transmitted to the host by Ixodes ticks. European ricinus ticks transmit different B. species, pathogenic human. Bacteria are principally present in unfed tick midgut, then migrate salivary glands during blood meal and infect new via saliva. In this study, efficiency transmission mouse model three species belonging complex, sensu stricto (B31, N40 BRE-13), afzelii (IBS-5) bavariensis (PBi) examined order evaluate infection risk after...
We hypothesized that the up-regulated expression of one or more members regulator G protein signaling (RGS) family can cause an attenuation via Gi/Go-coupled opioid receptors, and thereby play a role in development hyperalgesia accompanying insensitivity to morphine observed animal models neuropathic pain. Accordingly, we examined mRNA several RGS genes rat model chronic pain induced by partial ligation sciatic nerve. During hyperalgesia, RGS4 was only isoform whose levels increased...
ABSTRACT We determined the prevalence of gyrA mutations conferring fluoroquinolone resistance in 97 Helicobacter pylori isolates collected France from 2007 to 2010. Ninety-four harbored one or two already found quinolone determining region (QRDR) (for T87I, n = 23; for N87K, 32; D91N, 30; D91G, 7; D91Y, 6), 2 a mutation never previously described (D91H and A88P), strain was resistant (ciprofloxacin MIC 8 mg/liter) without detected this gyrB genes.
Lyme borreliosis is the most common tick-borne disease in northern hemisphere. In Europe, it transmitted by Ixodes ticks that carry bacteria belonging to Borrelia burgdorferi sensu lato complex. The objective of this work was explore eco-epidemiological factors peri-urban forests France (Sénart, Notre-Dame and Rambouillet). We investigated whether introduction Tamias sibiricus Sénart could alter density infected ticks. Moreover, tick infection were according tree species found various...
Pseudomonas aeruginosa, an opportunistic pathogen involved in skin and lung diseases, possesses numerous virulence factors, including type 2 3 secretion systems (T2SS T3SS) its flagellum, whose functions remain poorly known during cutaneous infection. Using isogenic mutants deleted from genes encoding each or all of these three we investigated their role induction inflammatory response tissue invasiveness human primary keratinocytes reconstructed epidermis. Our results showed that but not...
To evaluate the role of estrogen receptor in differentiation cells neural origin, we developed a molecular approach aimed at identification target genes by mRNA differential display PCR (ddPCR) human neuroblastoma SK-ER3 cells. More than 3000 RNAs were examined, few which displayed regulation pattern response to 17β-estradiol (E 2 ). Sequence analysis three differentially amplified ddPCR products showed homology with growth-associated nuclear protein prothymosin-α (PTMA), Bcl2-interacting...
We previously demonstrated that the mitochondrial peripheral-type benzodiazepine receptor (PBR) is coupled to hormone-activated steroidogenesis by regulating intramitochondrial cholesterol transport, rate-determining step of steroid biosynthesis. In present study we examined whether PBR site hormone action using hCG-responsive MA-10 mouse Leydig tumor cell line as a model system. Within 15 sec addition hCG cells 3-fold cAMP-dependent increase in binding was observed. This rapid returned...
Abstract We found that 20.5% of patients with an unexplained fever in northwestern Morocco had tick-borne relapsing fever. Molecular detection specific for the 16S rRNA gene identified Borrelia hispanica. The noncoding intergenic spacer sequence domain showed high sensitivity and good resolution this species.
Lyme borreliosis, one of the most frequently contracted zoonotic diseases in Northern Hemisphere, is caused by bacteria belonging to different genetic groups within Borrelia burgdorferi species complex, which are transmitted ticks among various wildlife reservoirs, such as small mammals and birds. These features make complex an attractive biological model that can be used study diversification epidemiology endemic bacterial pathogens. We investigated potential population genomic approaches...