Burkholderia pseudomallei is the causative agent of melioidosis, a frequently occurring disease in northeastern Thailand, where soil and water high salt content are common. Using microarray analysis, we previously showed that B. up-regulated short-chain dehydrogenase/oxidoreductase (SDO) under stress. However, importance SDO infection unknown. This study aimed to explore function SDO, investigate its role interactions between host cells. Bioinformatics analysis structure, based on homology...

Burkholderia pseudomallei is an intracellular pathogen and the causative agent of melioidosis, a life-threatening disease humans. Within host cells, superoxide important mediator killing. In this study, we have identified B. K96243 sodC gene, shown that it has dismutase activity, constructed allelic deletion mutant gene. Compared with wild-type, was more sensitive to killing by extracellular superoxide, but not generated intracellularly. The showed markedly decreased survival in J774A.1...

Bloodstream infection surveillance conducted from 2008 to 2014 in all 20 hospitals Sa Kaeo and Nakhon Phanom provinces, Thailand, allowed us look at disease burden, antibiotic susceptibilities, recurrent infections caused by extended-spectrum β-lactamase (ESBL)–producing Escherichia coli Klebsiella pneumoniae. Of 97,832 blood specimens, 3,338 were positive for E. 1,086 K. The proportion of isolates producing ESBL significantly increased 19% 22% 2008–2010 approximately 30% 2011 (P-value trend...

Acinetobacter baumannii is a priority bacterial pathogen and leading cause of nosocomial infections, particularly in intensive care units (ICUs). The average incidence carbapenem-resistant A. infections ICUs 41.7 cases/1,000 patients, highlighting the urgent need for more effective alternative therapies to replace carbapenems. Thus, this study aimed investigate first time antibacterial activity curcumin combination with novel phage vB_AbaSI_1 combat multidrug-resistant (MDR) vitro. Phage...

Trehalose is a disaccharide formed from two glucose molecules. This sugar molecule can be isolated range of organisms including bacteria, fungi, plants and invertebrates. has variety functions role as an energy storage molecule, structural component glycolipids plays in the virulence some microorganisms. There are many metabolic pathways that control biosynthesis degradation trehalose different organisms. The enzyme trehalase forms part pathway converts into glucose. In this study we set out...

Bacteriophages (phages), viruses that infect bacteria, are found in abundance not only the environment but also human body. The use of phages for diagnosis melioidosis, a tropical infectious disease caused by Burkholderia pseudomallei , is emerging as promising novel approach, our understanding conditions under which prophages can be induced remains limited. Here, we first demonstrated isolation from hemocultures melioidosis patients. B. -positive hemoculture bottles were filtered to remove...

Burkholderia pseudomallei, the causative agent of melioidosis, can be isolated from soil and water. To persist, adapt survive within outside their human host, bacteria rely on regulatory mechanisms that allow them to respond rapidly stressful situations. We have examined possible role B. pseudomallei alternative sigma factor sigma(E) (RpoE) in stress response found rpoE its putative regulators (bprE-rseB-mucD) are transcribed a single transcriptional unit. Inactivation operon changed...

We have previously shown that the alternative sigma factor sigmaE (RpoE), encoded by rpoE, is involved in stress tolerance and survival of Burkholderia pseudomallei. However, its molecular pathogenic mechanisms remain unclear. In present study, we applied gel-based, differential proteomics to compare cellular proteome an rpoE operon knockout mutant (RpoE Mut) wild-type (K96243 WT) B. Quantitative intensity analysis (n = 5 gels from individual culture flasks each group) revealed significantly...

Abstract Burkholderia pseudomallei is an environmental saprophyte and the causative agent of melioidosis, a severe infectious disease prevalent in tropical areas, including southeast Asia northern Australia. In Thailand, highest incidence melioidosis northeast region, where saline soil water are abundant. We hypothesized that B. develops ability to thrive conditions gains selective ecological advantage over other soil‐dwelling microorganisms. However, little known about how elevated NaCl...

Burkholderia pseudomallei is a gram-negative bacterium and the causative agent of melioidosis, one important lethal diseases in tropical regions. In this article, we demonstrate crucial role B. rpoE locus response to heat stress. The operon knockout mutant exhibited growth retardation reduced survival when exposed high temperature. Expression analysis using rpoH promoter-lacZ fusion revealed that stress induction rpoH, which encodes shock sigma factor (sigma(H)), was abolished mutant....

The twin arginine translocation (Tat) system in bacteria is responsible for transporting folded proteins across the cytoplasmic membrane, and some bacteria, Tat-exported substrates have been linked to virulence. We report here that Tat machinery present Burkholderia pseudomallei, B. mallei, thailandensis, we show essential aerobic but not anaerobic growth. Switching off of thailandensis grown anaerobically resulted filamentous showed increased sensitivity β-lactam antibiotics. In Galleria...

Domestic and wild animals are important reservoirs for antibiotic-resistant bacteria. This study aimed to isolate Escherichia coli from feces of domestic at an agricultural land interface area Salaphra Wildlife Sanctuary, Thailand, the phylogenic characteristics antibiotic resistance in these isolates. In this cross-sectional, descriptive study, we randomly collected ground free-ranging (deer elephants) (cattle goats). All fecal samples were inoculated onto MacConkey agar plates,...

ABSTRACT In this exploratory study, we developed an automated workflow that leverages Large Language Models, specifically GPT-4, to prioritize candidate genes for targeted assay development. The automates interaction with OpenAI models and enables prompt creation, submission. It features customizable prompts designed evaluate based on criteria such as association biological processes, biomarker potential, therapeutic implications, which can be tailored specific diseases or processes....

Leptospirosis remains an important worldwide zoonotic disease caused by Leptospira spp affecting human and animals. This research aims to study the virulent-associated secreted proteins (protein secretome) of pathogenic interrogans serovar Icterohemorrhagiae strain RGA (Leptospira RGA) transition from environment mammalian physiological osmolarity, temperature (37 °C) carbon dioxide concentration (5% CO2) conditions for 24 h. Mass Spectrometry bioinformatics approaches, we identified...

Burkholderia pseudomallei is a facultative intracellular bacterial pathogen that causes melioidosis, severe invasive disease of humans. We previously reported the stress-related catecholamine hormone epinephrine enhances motility B . , transcription flagellar genes and production flagellin. It has been QseBC two-component sensory system regulates virulence-associated in other Gram-negative bacteria response to catecholamines, albeit disparities between studies exist. constructed whole-genome...

Antimicrobial-resistant Enterobacterales carriage and the coronavirus disease 2019 (COVID-19) lockdown measures may impact incidence all-cause mortality rate among nursing home residents. To determine in presence/absence of antimicrobial-resistant before during COVID-19 pandemic lockdown, this prospective closed-cohort study was conducted at various types homes Bangkok, Thailand, from June 2020 to December 2021. The elderly residents included 142 participants (aged ≥60 years) living ≥3...

Abstract Background Acute flaccid paralysis (AFP) surveillance was conducted as part of the World Health Organization’s strategy for completely eradicating poliomyelitis and leaving non-polio enteroviruses NPEVs one main potential causes AFP. We aimed to detect NPEV in association with Methods used 459 isolates reported be Negative Polio some by Organization Regional Reference Laboratory (Thailand), which had been obtained during polio programmes Thailand 2013–2014. Of isolates, 35 belonged...

Abstract Background Acute flaccid paralysis (AFP) surveillance was conducted as part of the World Health Organization’s strategy for completely eradicating poliomyelitis and leaving non-polio enteroviruses NPEVs one main potential causes AFP. We aimed to detect NPEV in association with Methods used 459 isolates reported be Negative Polio some by Organization Regional Reference Laboratory (Thailand), which had been obtained during polio programmes Thailand 2013–2014. Of isolates, 35 belonged...