A5042042350- Bacteriophages and microbial interactions
- Genomics and Phylogenetic Studies
- Viral gastroenteritis research and epidemiology
- Clostridium difficile and Clostridium perfringens research
- Microbial Community Ecology and Physiology
- Microbial infections and disease research
- Plant Virus Research Studies
- Genetics, Bioinformatics, and Biomedical Research
- Salmonella and Campylobacter epidemiology
- Monoclonal and Polyclonal Antibodies Research
- Burkholderia infections and melioidosis
- CRISPR and Genetic Engineering
- Vector-borne infectious diseases
- Protist diversity and phylogeny
- Viral Infections and Vectors
- Antibiotic Resistance in Bacteria
- Aquaculture disease management and microbiota
- Respiratory viral infections research
- RNA and protein synthesis mechanisms
- Vibrio bacteria research studies
- Biomedical and Engineering Education
- Gut microbiota and health
- Cancer Research and Treatments
- Mosquito-borne diseases and control
- Insect symbiosis and bacterial influences
University of Leicester
2016-2025
Umm al-Qura University
2024
North Bristol NHS Trust
2024
Southmead Hospital
2024
Royal Brompton Hospital
2019
Imperial College London
2019
Ludwig-Maximilians-Universität München
2019
German Center for Lung Research
2019
Helmholtz Zentrum München
2019
Institut thématique Immunologie, inflammation, infectiologie et microbiologie
2011-2014
Phages are the most abundant organisms in biosphere and they a ubiquitous feature of prokaryotic existence. A bacteriophage is virus which infects bacterium. Archaea also infected by viruses, whether these should be referred to as ‘phages’ debatable, but included such scope this article. Phage have been interest scientists tools understand fundamental molecular biology, vectors horizontal gene transfer drivers bacterial evolution, sources diagnostic genetic tools, novel therapeutic agents....
Background: With advances in sequencing technology and decreasing costs, the number of phage genomes that have been sequenced has increased markedly past decade. Materials Methods: We developed an automated retrieval analysis system for (https://github.com/RyanCook94/inphared) to produce INfrastructure a PHAge REference Database (INPHARED) associated metadata. Results: As January 2021, 14,244 complete sequenced. The INPHARED data set is dominated by phages infect small bacterial genera, with...
The microbiome dysbiosis caused by antibiotic treatment has been associated with both susceptibility to and relapse of Clostridium difficile infection (CDI). Bacteriophage (phage) therapy offers target specificity dose amplification in situ, but few studies have focused on its use CDI treatment. This mainly reflects the lack strictly virulent phages that this pathogen. While it is widely accepted temperate are unsuitable for therapeutic purposes due their transduction potential, analysis...
The characterization of therapeutic phage genomes plays a crucial role in the success rate therapies. There are three checkpoints that need to be examined for selection candidates, namely, presence temperate markers, antimicrobial resistance (AMR) genes, and virulence genes. However, currently, no single-step tools available this purpose. Hence, we have developed tool capable checking all conditions required suitable candidates. This consists an ensemble machine-learning-based predictors...
Bacterial defense against phage predation involves diverse systems acting individually and concurrently, yet their interactions remain poorly understood. We investigated >100 in 42,925 bacterial genomes identified numerous instances of non-random co-occurrence negative association. For several pairs significantly co-occurring Escherichia coli strains, we demonstrate synergistic anti-phage activity. Notably, Zorya II synergizes with Druantia III ietAS systems, while tmn exhibits synergy...
Viral metagenomics has fuelled a rapid change in our understanding of global viral diversity and ecology. Long-read sequencing hybrid assembly approaches that combine long- short-read technologies are now being widely implemented bacterial genomics metagenomics. However, the use long-read to investigate communities is still its infancy. While Nanopore PacBio have been applied metagenomics, it not known what extent different will impact reconstruction community. Thus, we constructed mock...
Bacteriophage S-PM2 infects several strains of the abundant and ecologically important marine cyanobacterium Synechococcus. A large lytic phage with an isometric icosahedral head, has a contractile tail by this criterion is classified as myovirus (1). The linear, circularly permuted, 196,280-bp double-stranded DNA genome contains 37.8% G+C residues. It encodes 239 open reading frames (ORFs) 25 tRNAs. Of these ORFs, 19 appear to encode proteins associated cell envelope, including putative...
The discovery of the genes psbA and psbD , encoding D1 D2 core components photosynthetic reaction center PSII (photosystem II), in genome bacteriophage S-PM2 (a cyanomyovirus) that infects marine cyanobacteria begs question as to how these were acquired. In an attempt answer this question, it was established occurrence is widespread among cyanomyovirus isolates may even extend podoviruses. phage fall into a clade includes from their potential Synechococcus Prochlorococcus hosts, thus,...
The transfer of novel genetic material into the genomes bacterial viruses (phages) has been widely documented in several host-phage systems. Bacterial genes are incorporated phage genome and, if retained, subsequently evolve within them. expression these can subvert or bolster processes, including altering pathogenicity. phiCDHM1 infects Clostridium difficile, a pathogenic bacterium that causes nosocomial infections and is associated with antibiotic treatment. Genome sequencing annotation...
Abstract Bacteriophage therapeutic development will clearly benefit from understanding the fundamental dynamics of in vivo phage-bacteria interactions. Such information can inform animal and human trials, much be ascertained cell-line work. We have developed a cell-based system using Clostridium difficile , pernicious hospital pathogen with limited treatment options, phage phiCDHS1 that effectively kills this bacterium liquid culture. The colon tumorigenic cell line HT-29 was used because it...
Clostridium difficile infection (CDI) is a global health threat associated with high rates of morbidity and mortality. Conventional antibiotic CDI therapy can result in treatment failure recurrent infection. C. produces biofilms which contribute to its virulence impair antimicrobial activity. Some bacteriophages (phages) penetrate thus could be developed either replace or supplement antibiotics. Here, we determined the impact previously optimized 4-phage cocktail on ribotype 014/020...
Antimicrobial resistance (AMR) is a major problem globally. The main bacterial organisms associated with urinary tract infection (UTI) sepsis are E. coli and Klebsiella along Enterobacter species. These all have AMR strains known as ESBL (Extended Spectrum Beta-Lactamase), which featured on the WHO priority pathogens list “critical” for research. Bacteriophages (phages), viruses that can infect kill bacteria, could provide an effective tool to tackle these strains. There currently no “gold...