A5013689373- Bacteriophages and microbial interactions
- Evolution and Genetic Dynamics
- Genomics and Phylogenetic Studies
- Plant Virus Research Studies
- Antibiotic Resistance in Bacteria
- Microbial Community Ecology and Physiology
- CRISPR and Genetic Engineering
- Bacterial Genetics and Biotechnology
- Virus-based gene therapy research
- Evolutionary Game Theory and Cooperation
- Antibiotics Pharmacokinetics and Efficacy
- RNA Interference and Gene Delivery
- Monoclonal and Polyclonal Antibodies Research
- Extracellular vesicles in disease
- Origins and Evolution of Life
- Plant and animal studies
- Respiratory viral infections research
- Microbial infections and disease research
- Plant and Fungal Interactions Research
- Plant Disease Resistance and Genetics
- Nanocluster Synthesis and Applications
- Gold and Silver Nanoparticles Synthesis and Applications
- Insect and Pesticide Research
- Bacillus and Francisella bacterial research
- Viral Infections and Vectors
University of Jyväskylä
2014-2023
University of Cambridge
2018-2019
University of Helsinki
2012-2018
Australian National University
2012-2014
Bacteriophages are bacterial viruses, capable of killing even multi-drug resistant cells. For this reason, therapeutic use phages is considered as a possible alternative to conventional antibiotics. However, very host specific in comparison wide-spectrum antibiotics and thus preparation phage-cocktails beforehand against pathogens can be difficult. In study, we evaluate whether it may isolate on-demand from environmental reservoir. We attempted enrich infectious bacteriophages sewage...
Standard of care for cancer is commonly a combination surgery with radiotherapy or chemoradiotherapy. However, in some advanced patients this approach might still remaininefficient and may cause many side effects, including severe complications even death. Oncolytic viruses exhibit different anti-cancer mechanisms compared conventional therapies, allowing the possibility improved effect therapy. Chemotherapeutics combined oncolytic stronger cytotoxic responses oncolysis. Here, we have...
Bacteriophage therapy, the use of viruses that infect bacteria as antimicrobials, has been championed a promising alternative to conventional antibiotics. Although in laboratory bacterial resistance against phages arises rapidly, so far an only minor problem for effectiveness phage therapy. Resistance antibiotics, however, become major issue after decades extensive use. Should we expect similar problems long-term antimicrobials? Like are often noted be drivers evolution. phage-treated...
Antibiotic-resistance genes are often carried by conjugative plasmids, which spread within and between bacterial species. It has long been recognized that some viruses of bacteria (bacteriophage; phage) have evolved to infect kill plasmid-harbouring cells. This raises a question: can phages cause the loss plasmid-associated antibiotic resistance selecting for plasmid-free bacteria, or plasmids evolve in other ways? Here, we show multiple antibiotic-resistance containing stably maintained...
The emergence of pathogenic bacteria resistant to multiple antibiotics is a serious worldwide public health concern. Whenever are applied, the genes encoding for antibiotic resistance selected within bacterial populations. This has led prevalence conjugative plasmids that carry and can transfer themselves between diverse groups. In this study, we investigated whether it feasible attempt prevent spread resistances with lytic bacteriophage, which replicate in wide range gram-negative...
The coincidental evolution hypothesis predicts that traits connected to bacterial pathogenicity could be indirectly selected outside the host as a correlated response abiotic environmental conditions or different biotic species interactions. To investigate this, an opportunistic pathogen, Serratia marcescens, was cultured in absence and presence of lytic bacteriophage PPV (Podoviridae) at 25°C 37°C for four weeks (N = 5). At end, we measured changes phage-resistance potential virulence...
Extracellular vesicles (EVs) have been showcased as auspicious candidates for delivering therapeutic cargo, including oncolytic viruses cancer treatment. Delivery of in EVs could provide considerable advantages, hiding the from immune system and providing alternative entry pathways into cells. Here we describe formation viral cargo secreted by cells infected with an adenovirus (IEVs, cell-derived EVs) a function time after infection. IEVs were already before lytic release virions their...
In this article, we - the Bacterial Viruses Subcommittee and Archaeal of International Committee on Taxonomy (ICTV) summarise results our activities for period March 2020 2021. We report division former in two separate Subcommittees, welcome new members, a Chair Vice Chair, give an overview taxa that were proposed 2020, approved by Executive ratified vote particular, realm, three orders, 15 families, 31 subfamilies, 734 genera 1845 species newly created or redefined (moved/promoted).
Studies on viral capsid architectures and coat protein folds have revealed the evolutionary lineages of viruses branching to all three domains life. A widespread group icosahedral tailless viruses, PRD1-adenovirus lineage, was first be established. double β-barrel fold for a single major is characteristic these viruses. Similar carrying genes coding two proteins with more complex structure, such as Thermus phage P23-77 haloarchaeal virus SH1, been isolated. Here, we studied host range, life...
Sub-minimum inhibiting concentrations (sub-MICs) of antibiotics frequently occur in natural environments owing to wide-spread antibiotic leakage by human action. Even though the are very low, these sub-MICs have recently been shown alter bacterial populations selecting for resistance and increasing rate adaptive evolution. However, studies lacking on how effects reverberate into key ecological interactions, such as bacteria–phage interactions. Previously, co-selection bacteria phages...
Prokaryotes harbor a variety of genetic replicators, including plasmids, viruses, and chromosomes, each having different effects on the phenotype hosting cell. Here, we propose classification for replicators bacteria archaea basis their horizontal‐transfer potential type relationships (mutualistic, symbiotic, commensal, or parasitic) that they have with host cell vehicle. Horizontal movement can be either active passive, reflecting whether not replicator encodes means to mediate its own...
Water-soluble gold nanoclusters with well-defined molecular structures and stability possess particular biophysical properties making them excellent candidates for biological applications as well fundamental spectroscopic studies. The currently existing synthetic protocols atomically monodisperse thiolate-protected (AuMPCs) have been widely expanded organothiolates, yet the direct synthesis reports water-soluble AuMPCs are still deficient. Here, we demonstrate a wet-chemistry pH-controlled...
The possibility to modify gut bacterial flora has become an important goal, and various approaches are used achieve desirable communities. However, the genetic engineering of existing microbes in gut, which already compatible with rest community host immune system, not received much attention. Here, we discuss experimentally evaluate use modified mobilizable CRISPR-Cas9-endocing plasmid as a tool induce changes This system (briefly midbiotic) is delivered from vector into target bacteria via...
The human gut microbiome is composed of diverse microbes, and its association with health well-recognized. Antibiotic therapies for treating infectious diseases often mediate adverse influences on microbial ecosystems. Further, ESBL (extended-spectrum beta-lactamase)-producing bacteria potentially residing in the benefit from antibiotic-induced environmental changes via positive selection resistance traits. As antimicrobial (AMR) genes are harbored by mobile plasmids, importance these...
Abstract Bacteria often acquire resistance against antibiotics through the transfer of conjugative plasmids. Hence, it is vital to develop strategies mitigate dispersal antimicrobial (AMR). CRISPR-based tools offer a sequence-specific solution diminish and restrict dissemination genes among bacteria. CRICON (CRISPR via conjugation) an CRISPR tool that has been shown efficiently reduce multi-resistance when targeting ESBL (Extended Spectrum Beta-Lactamase) harboring However, conjugatively...
ABSTRACT The genome sequence of a Bacillus anthracis -specific clear plaque mutant phage, AP50c, contains 31 open reading frames spanning 14,398 bp, has two mutations compared to wild-type AP50t, and colinear architecture highly similar that gram-positive Tectiviridae phages. Spontaneous AP50c-resistant B. mutants exhibit mucoid colony phenotype.
ABSTRACT We have sequenced the genome and identified structural proteins lipids of novel membrane-containing, icosahedral virus P23-77 Thermus thermophilus . has an ∼17-kb circular double-stranded DNA genome, which was annotated to contain 37 putative genes. Virions were subjected dissociation analysis, five protein species shown associate with internal viral membrane, while three constituents capsid. Analysis bacteriophage revealed it be evolutionarily related another phage (IN93), archaeal...
Horizontal gene transfer by conjugative plasmids plays a critical role in the evolution of antibiotic resistance. Interactions between bacteria and other organisms can affect persistence spread plasmids. Here we show that protozoan predation increased resistance plasmid RP4 populations opportunist bacterial pathogen Serratia marcescens . A conjugation-defective mutant was unable to survive under predation, suggesting is required for realistic condition predation. These results indicate...
Prokaryotic biosphere is vastly diverse in many respects. Any given bacterial cell may harbor different combinations viruses, plasmids, transposons, and other genetic elements along with their chromosome(s). These agents interact complex environments various ways causing multitude of phenotypic effects on hosting cells. In this discussion I perform a dissection for order to simplify the diversity into components that help approach ocean details evolving microbial worlds. The itself separated...
Distribution of species across the Earth shows strong latitudinal and altitudinal gradients with number decreasing declining temperatures. While these patterns have been recognized for well over a century, mechanisms generating maintaining them remained elusive. Here, we propose mechanistic explanation temperature‐dependent rates molecular evolution that can influence speciation global biodiversity gradients. Our hypothesis is based on effects temperature temperature‐adaptation stability...
Bacteria live in dynamic systems where selection pressures can alter rapidly, forcing adaptation to the prevailing conditions. In particular, bacteriophages and antibiotics of anthropogenic origin are major bacterial stressors many environments. We previously observed that populations bacterium Pseudomonas fluorescens SBW25 exposed lytic bacteriophage SBW25Φ2 a noninhibitive concentration antibiotic streptomycin (coselection) achieved higher levels phage resistance compared alone. addition,...