A5055301255- Bacteriophages and microbial interactions
- Genomics and Phylogenetic Studies
- DNA and Nucleic Acid Chemistry
- Microbial Community Ecology and Physiology
- DNA Repair Mechanisms
- Enzyme Structure and Function
- Genomics and Chromatin Dynamics
- Bacterial Genetics and Biotechnology
- RNA and protein synthesis mechanisms
- Plant Virus Research Studies
- Cancer therapeutics and mechanisms
- Chromosomal and Genetic Variations
- Methane Hydrates and Related Phenomena
- RNA modifications and cancer
- Advanced biosensing and bioanalysis techniques
- Epigenetics and DNA Methylation
- Protist diversity and phylogeny
- Biochemical and Molecular Research
- Antibiotic Resistance in Bacteria
- Plant Water Relations and Carbon Dynamics
- Viral gastroenteritis research and epidemiology
- Plant Molecular Biology Research
- Plant responses to water stress
- Microbial infections and disease research
- Enzyme Production and Characterization
Institute of Microbiology
2016-2025
Chinese Academy of Sciences
2016-2025
Microbiology Institute of Shaanxi
2016-2025
University of Chinese Academy of Sciences
2016-2025
Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou)
2025
Institute of Subtropical Agriculture
2022-2024
Zhanjiang Experimental Station
2022-2024
Institute of Biophysics
2002-2024
Czech Academy of Sciences, Institute of Microbiology
1998-2023
National Human Genome Research Institute
2023
The histone variants H3.3 and H2A.Z have recently emerged as two of the most important features in transcriptional regulation, molecular mechanism which still remains poorly understood. In this study, we investigated regulation on chromatin dynamics during activation. Our vitro biophysical biochemical investigation showed that promoted compaction repressed activity. Surprisingly, with only four to five amino acid differences from canonical H3, greatly impaired higher-ordered folding gene...
ABSTRACT The bovine rumen represents a highly specialized bioreactor where plant cell wall polysaccharides (PCWPs) are efficiently deconstructed via numerous enzymes produced by resident microorganisms. Although large number of fibrolytic genes from microorganisms have been identified, it remains unclear how they expressed in coordinated manner to degrade PCWPs. In this study, we performed metatranscriptomic analysis the microbiomes adult Holstein cows fed fiber diet and obtained total...
Thermoanaerobacter tengcongensis is a rod-shaped, gram-negative, anaerobic eubacterium that was isolated from freshwater hot spring in Tengchong, China. Using whole-genome-shotgun method, we sequenced its 2,689,445-bp genome an isolate, MB4 T (Genbank accession no. AE008691 ). The encodes 2588 predicted coding sequences (CDS). Among them, 1764 (68.2%) are classified according to homology other documented proteins, and the rest, 824 CDS (31.8%), functionally unknown. One of interesting...
ABSTRACT The genomes of two Sulfolobus islandicus strains obtained from Icelandic solfataras were sequenced and analyzed. Strain REY15A is a host for versatile genetic toolbox. It exhibits genome minimal size, stable genetically, easy to grow manipulate. HVE10/4 shows broad range exceptional crenarchaeal viruses conjugative plasmids was selected studying their life cycles interactions. are 2.5 2.7 Mb, respectively, each carries variable region 0.5 0.7 Mb where major differences in gene...
ABSTRACT A virus infecting the hyperthermophilic archaeon Sulfolobus tengchongensis has been isolated from a field sample Tengchong, China, and characterized. The virus, denoted STSV1 ( spindle-shaped 1), morphology of spindle (230 by 107 nm) with tail variable length (68 nm on average) at one end is largest known viruses. After its host, multiplied rapidly to high titers (>10 10 PFU/ml). Replication retarded host growth but did not cause lysis cells. integrate into chromosome existed in...
The phylum Actinobacteria has been reported to be common or even abundant in deep marine sediments, however, knowledge about the diversity, distribution, and function of actinobacteria is limited. In this study, actinobacterial diversity sea along Southwest Indian Ridge (SWIR) was investigated using both 16S rRNA gene pyrosequencing culture-based methods. samples were collected at depths 1662–4000 m below water surface. Actinobacterial sequences represented 1.2–9.1% all microbial amplicon...
Specific recognition of centromere-specific histone variant CENP-A-containing chromatin by CENP-N is an essential process in the assembly kinetochore complex at centromeres prior to mammalian cell division. However, mechanisms recruitment centromeres/kinetochores remain unknown. Here, we show that a CENP-A-specific RG loop (Arg80/Gly81) plays and dual regulatory role this process. The assists formation compact "ladder-like" structure CENP-A chromatin, concealing thus impairing its recruiting...
Archaea contain a variety of chromatin proteins consistent with the evolution different genome packaging mechanisms. Among two main kingdoms in Archaea, Euryarchaeota synthesize histone homologs, whereas Crenarchaeota have not been shown to possess protein conserved at kingdom level. We report identification Cren7, novel family highly Crenarchaeota. A small, basic, methylated and abundant protein, Cren7 displays higher affinity for double-stranded DNA than single-stranded DNA, constrains...
In this article, we - the Bacterial Viruses Subcommittee and Archaeal of International Committee on Taxonomy (ICTV) summarise results our activities for period March 2020 2021. We report division former in two separate Subcommittees, welcome new members, a Chair Vice Chair, give an overview taxa that were proposed 2020, approved by Executive ratified vote particular, realm, three orders, 15 families, 31 subfamilies, 734 genera 1845 species newly created or redefined (moved/promoted).
Archaeal viruses are well known for their diverse morphologies and extreme stability. In this study, we used cryo-electron tomography to analyze the structure of SEV1 its infection strategies in native state. The results show that nucleocapsid adopts a ‘coil-stacking’ architecture which displays degree flexibility. VP4, whose homologues widespread thermo-acidic environment globally, is identified as major capsid protein binds genomic DNA forming “beads-on-a-string” arrangement. Simulations...
Abstract Seamounts are critical marine biodiversity hot spots, while the metabolic activity of their microbial community remains largely unknown. In this study, we investigated diversity and free-living particle-attached microorganisms in surface, middle, bottom layers seawater at Zhenbei seamount South China Sea using omics approaches including 16S rRNA/16S rDNA ratio analysis. Over 20 phyla were detected with Proteobacteria, Actinobacteriota, Cyanobacteria, Bacteroidota, Thaumarchaeota,...
Organisms belonging to the Crenarchaeota lineage contain three proliferating cell nuclear antigen (PCNA) subunits, while those in Euryarchaeota have only one, as for Eukarya. To study mechanism of archaeal sliding clamps, we sought generate knockouts each pcna gene Sulfolobus islandicus, a hyperthermophilic crenarchaeon, but failed with two conventional knockout methods. Then, new scheme, known marker insertion and target deletion (MID), was developed, which transformants were obtained...
An improved microfluidic streak plate technique relying on droplet microfluidics can advance the exploration of deep-sea rare microbes.
The phylum Gemmatimonadota is widely distributed in various environments. However, their physiology, ecology and evolutionary history remain unknown, primary due to the limited cultured isolates available genomes.
The DNA binding protein Ssh10b, a member of the Sac10b family, has been purified from hyperthermophilic archaeon Sulfolobus shibatae. Ssh10b constitutes about 4% cellular protein. Electrophoretic mobility shift assays showed that first bound double-stranded fragment with an estimated size approximately 12 bp, forming distinct shifts, until was coated Binding more resulted in formation smears lower mobilities. migration pattern smearing Ssh10b-DNA complexes affected by temperature, whereas...
Proteins of the Sac10b family, which is highly conserved among hyperthermophilic archaea, have been regarded as DNA-binding proteins. Based on their in vitro properties, these proteins are thought to be involved chromosomal organization or DNA repair/recombination. We show that Ssh10b, a member family from Sulfolobus shibatae, bound with similar affinities double-stranded DNA, single-stranded and RNA vitro. However, protein was exclusively S. shibatae cells, revealed by vivo UV cross-linking...
Abstract Thermus strains are regarded as models to investigate the mechanism of thermostability thermophiles, and phages from particularly interesting because their way regulate gene expression. In this research, a bacteriophage named TSP4 ( Siphoviridae phage) was isolated Tengchong hot springs in China, characteristics morphology, temperature for phage production, pH organic solvent sensitivity, DNA restriction endonuclease digestion protein composition were further studied. belonged...
Cren7, a newly found chromatin protein, is highly conserved in the Crenarchaeota. The protein shows higher affinity for double-stranded DNA than single-stranded DNA, constrains negative supercoils vitro and associated with genomic vivo. Here we report crystal structures of Cren7 from Sulfolobus solfataricus complex two sequences. binds minor groove causes single-step sharp kink (∼53°) through intercalation hydrophobic side chain Leu28. Loop β3-β4 undergoes significant conformational change...
Pathogens have evolved sophisticated mechanisms to evade detection and destruction by the host immune system. Large DNA viruses encode homologues of chemokines their receptors, as well chemokine-binding proteins (CKBPs) modulate chemokine network in response. The SECRET domain (smallpox virus-encoded receptor) represents a new family viral CKBPs that binds subset from different classes inhibit activities, either independently or fused with tumor necrosis factor receptors (vTNFRs). Here we...
Parasitic wasps rely on olfaction to locate their hosts in complex chemical environments. Odorant receptors (ORs) function together with well-conserved odorant coreceptors (ORcos) determine the sensitivity and specificity of olfactory reception. Campoletis chlorideae (Hymenoptera: Ichneunmonidae) is a solitary larval endoparasitoid cotton bollworm, Helicoverpa armigera, some other noctuid species. To understand molecular basis C. chlorideae's reception, we sequenced transcriptome adult male...