A5000938142- Genomics and Phylogenetic Studies
- Bacterial Genetics and Biotechnology
- Bacteriophages and microbial interactions
- Enzyme Structure and Function
- RNA and protein synthesis mechanisms
- Nuclear Materials and Properties
- Microbial Community Ecology and Physiology
- Fatigue and fracture mechanics
- Enzyme Production and Characterization
- Fusion materials and technologies
- High Temperature Alloys and Creep
- Biochemical and Molecular Research
- Turtle Biology and Conservation
- Physiological and biochemical adaptations
- DNA Repair Mechanisms
- Cytomegalovirus and herpesvirus research
- Hydrogen embrittlement and corrosion behaviors in metals
- RNA Interference and Gene Delivery
- Tea Polyphenols and Effects
- Non-Destructive Testing Techniques
- Food Quality and Safety Studies
- CRISPR and Genetic Engineering
- Plant Virus Research Studies
- Advanced Materials Characterization Techniques
- Fermentation and Sensory Analysis
Tongji Hospital
2022-2025
Huazhong University of Science and Technology
2022-2025
Nanjing Normal University
2023-2025
University of Illinois Urbana-Champaign
2015-2024
Shantou University Medical College
2019-2024
Second Affiliated Hospital of Shantou University Medical College
2017-2024
Shantou University
2017-2024
China Institute of Atomic Energy
2000-2023
Sichuan Agricultural University
2021-2022
Heilongjiang University of Technology
2018
Arid and semi-arid regions comprise nearly one-fifth of the earth's terrestrial surface. However, diversities functions their soil microbial communities are not well understood, despite ecological importance in driving biogeochemical cycling. Here, we analyzed geochemistry desert soils from Tarim Basin, northwestern China. Our geochemical data indicated half these saline. Metagenomic analysis showed that bacterial phylotypes (89.72% on average) dominated community, with relatively small...
Abstract Sulfolobus islandicus is a model microorganism in the TACK superphylum of Archaea, key lineage evolutionary history cells. Here we report genome-wide identification repertoire genes essential to S. growth culture. We confirm previous targeted gene knockouts, uncover non-essentiality functions assumed be cell, including proteinaceous S-layer, and highlight whose are yet determined. Phyletic distributions illustrate potential transitions that may have occurred during evolution this...
Abstract Different responses or tolerance to thermal stress between invasive and native species can affect the outcome of interactions climate change biological invasion. However, knowledge about physiological mechanisms that modulate interspecific differences in is limited. The present study analyzes metabolic by globally turtle, Trachemys scripta elegans , as compared with two co‐occurring turtle China, Pelodiscus sinensis Mauremys reevesii . Changes metabolite contents expression enzyme...
We investigated the interaction between Sulfolobus spindle-shaped virus (SSV9) and its native archaeal host islandicus. show that upon exposure to SSV9, S. islandicus strain RJW002 has a significant growth delay where majority of cells are dormant (viable but not growing) for 24 48 hours postinfection (hpi) compared controls without virus. demonstrate in this system, dormancy (i) is induced by both active inactive particles at low multiplicity infection (MOI), (ii) reversible strains with...
As a type of "cold tumor" with limited immune cell infiltration, ovarian cancer has historically shown efficacy in immunotherapy. In this study, we report that exosomes from can specifically target omentum which is the predilection site for to metastasize and combat subsequently implanted tumor. Furthermore, found substantial increase proportion CD3 + T cells, particularly CD8 within omental tissue where homed. This was accompanied by significant enhancement granzyme B levels cells....
Greater thermal tolerance of invasive species benefits their survival and spread under extreme climate events, especially global warming. Revealing the mechanisms underlying interspecific differences in between native can help understand invasion process predict potential invaders. Here, we link changes transcriptomics antioxidant defense at multiple temperatures with limits juveniles a successful globally turtle, Trachemys scripta elegans, turtle China, Mauremys reevesii. The two show...
Sulfolobus islandicus, an emerging crenarchaeal model organism, offers unique advantages for metabolic engineering and synthetic biology applications owing to its ability thrive in extreme environments. Although several genetic tools have been established this the lack of well-characterized chromosomal integration sites has considerably limited potential as a cellular factory. Here, we systematically identified characterized 13 artificial CRISPR RNAs (crRNAs) targeting eight S. islandicus...
Organisms belonging to the Crenarchaeota lineage contain three proliferating cell nuclear antigen (PCNA) subunits, while those in Euryarchaeota have only one, as for Eukarya. To study mechanism of archaeal sliding clamps, we sought generate knockouts each pcna gene Sulfolobus islandicus, a hyperthermophilic crenarchaeon, but failed with two conventional knockout methods. Then, new scheme, known marker insertion and target deletion (MID), was developed, which transformants were obtained...
We report here a novel selectable marker for the hyperthermophilic crenarchaeon Sulfolobus islandicus. The cassette is composed of sac7d promoter and hmg gene coding 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase (P(sac7d)-hmg), which confers simvastatin resistance to this crenarchaeon. basic plasmid vector pSSR was constructed by substituting pyrEF expression pSeSD P(sac7d)-hmg with plasmids pSSRlacS, pSSRAherA, pSSRNherA were constructed. Characterization transformants carrying...
BACKGROUND The aim of this study was to investigate the association between body mass index (BMI) and brachial-ankle pulse wave velocity (baPWV) in hypertensive males. MATERIAL AND METHODS Altogether, 14 866 male participants were included analysis. Participants divided into 3 groups: low BMI group (BMI <24 kg/m²), moderate (24 kg/m² ≤BMI <28 high ≥28 kg/m²). According baPWV values, arteriosclerosis (AS) set as degrees: mild AS (baPWV ≥1400 cm/s), ≥1700 cm/s) severe ≥2000 cm/s). Multivariate...
The S-layer is considered to be the sole component of cell wall in Sulfolobales , a taxonomic group within Crenarchaeota whose cellular features have been suggested close relationship last archaea-eukaryote common ancestor. In this study, we genetically dissect how two previously characterized genes as well newly identified S-layer-associated protein-encoding gene contribute architecture Sulfolobus . We provide genetic evidence for first time showing that slaA key morphology determinant and...
Sulfolobus species have become the model organisms for studying unique biology of crenarchaeal division archaeal domain. In particular, islandicus provides a powerful opportunity to explore natural variation via experimental functional genomics. To support these efforts, we further expanded genetic tools S. by developing stringent positive selection agmatine prototrophs in strains which argD gene, encoding arginine decarboxylase, has been deleted. Strains with deletions were shown be...
The identification of microbial genes essential for survival as those with lethal knockout phenotype (LKP) is a common strategy functional interrogation genomes. However, interpretation the LKP complicated because substantial fraction this remains poorly functionally characterized. Furthermore, many can exhibit not their products perform cellular functions but activates toxicity other (conditionally genes). We analyzed sets two archaea,
Sulfolobus species have been developed as excellent model organisms to address fundamental questions of archaeal biology. Interesting patterns natural variation among islandicus strains identified through genome sequencing. Experimentally testing hypotheses about the biological causes and consequences this requires genetic tools that apply a diversity strains. Previously, transformation system for S. was reported, in which overexpression 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA)...