A5071726265- Bacteriophages and microbial interactions
- RNA and protein synthesis mechanisms
- Plant Virus Research Studies
- Plant and Fungal Interactions Research
- RNA Interference and Gene Delivery
- Bacterial Genetics and Biotechnology
- Genomics and Phylogenetic Studies
- Viral gastroenteritis research and epidemiology
- interferon and immune responses
- Microbial infections and disease research
- Influenza Virus Research Studies
- Herpesvirus Infections and Treatments
- Viral Infections and Immunology Research
- Virus-based gene therapy research
- Field-Flow Fractionation Techniques
- Immune Response and Inflammation
- Respiratory viral infections research
- Plant-Microbe Interactions and Immunity
- RNA modifications and cancer
- Chromosomal and Genetic Variations
- Animal Virus Infections Studies
- Hepatitis C virus research
- Immunotherapy and Immune Responses
- Protein purification and stability
- SARS-CoV-2 and COVID-19 Research
University of Helsinki
2016-2025
University of Giessen
2021
Helsinki Art Museum
2021
Biocenter Finland
2006-2016
Diamond Light Source
2013
Centre for Human Genetics
2013
Šiauliai University
2013
Institute for Molecular Medicine Finland
2013
University of Oxford
2013
BD Biosciences (United States)
2012
Abstract This article summarises the activities of Bacterial Viruses Subcommittee International Committee on Taxonomy for period March 2021−March 2022. We provide an overview new taxa proposed in 2021, approved by Executive Committee, and ratified vote Significant changes to taxonomy bacterial viruses were introduced: paraphyletic morphological families Podoviridae , Siphoviridae Myoviridae as well order Caudovirales abolished, a binomial system nomenclature species was established. In...
Electron cryomicroscopy can yield near-atomic resolution structures of highly ordered macromolecular complexes. Often however some subunits bind in a flexible manner, have different symmetry from the rest complex, or are present sub-stoichiometric amounts, limiting attainable resolution. Here we report general method for localized three-dimensional reconstruction such subunits. After determining particle orientations, local areas corresponding to be extracted and treated as single particles....
A universal taxonomy of viruses is essential for a comprehensive view the virus world and communicating complicated evolutionary relationships among viruses. However, there are major differences in conceptualisation approaches to classification nomenclature virologists, clinicians, agronomists, other interested parties. Here, we provide recommendations guide construction coherent taxonomy, based on expert scientific consensus. Firstly, assignments should be congruent with best attainable...
In vertebrates and plants, dsRNA plays crucial roles as PAMP a mediator of RNAi. How higher fungi respond to is not known. We demonstrate that Magnaporthe oryzae (Mo), globally significant crop pathogen, internalizes across broad size range 21 about 3000 bp. Incubation fungal conidia with 10 ng/µL dsRNA, regardless or sequence, induced aberrant germ tube elongation, revealing strong sequence-unspecific effect in this fungus. Accordingly, the synthetic analogue poly(I:C) various sizes...
Summary Pathogens induce severe damages on cultivated plants and represent a serious threat to global food security. Emerging strategies for crop protection involve the external treatment of with double‐stranded (ds) RNA trigger interference. However, applying this technology in greenhouses fields depends ds quality, stability efficient large‐scale production. Using components bacteriophage phi6, we engineered stable accurate vivo production system Pseudomonas syringae bacteria. Unlike other...
The RNAP elongation dynamic of dsRNA virus is captured by magnetic tweezers d Large sets high-resolution data allow quantification polymerase fidelity Maximum likelihood estimation allows extraction critical model parameters
The discovery of RNA interference (RNAi) has revolutionized biological research and a huge potential for therapy. Since small double-stranded RNAs (dsRNAs) are required various RNAi applications, there is need cost-effective methods producing large quantities high-quality dsRNA. We present two novel, flexible virus-based systems the efficient production dsRNA: (1) an in vitro system utilizing combination T7 polymerase RNA-dependent (RdRP) bacteriophage ϕ6 to generate dsRNA molecules...
The production of aberrant RNA (aRNA) is the initial step in several RNAi pathways. How aRNA produced and specifically recognized by RNA-dependent polymerases (RdRPs) to generate double-stranded (dsRNA) not clear. We previously showed that filamentous fungus Neurospora, RdRP QDE-1 required for rDNA-specific production, suggesting may be important synthesis. Here we show a recombinant both an DNA-dependent polymerase (DdRP). Its DdRP activity much more robust than occurs on ssDNA but dsDNA...
In this article, we - the Bacterial Viruses Subcommittee and Archaeal of International Committee on Taxonomy (ICTV) summarise results our activities for period March 2020 2021. We report division former in two separate Subcommittees, welcome new members, a Chair Vice Chair, give an overview taxa that were proposed 2020, approved by Executive ratified vote particular, realm, three orders, 15 families, 31 subfamilies, 734 genera 1845 species newly created or redefined (moved/promoted).
RNA viruses are the fastest evolving known biological entities. Consequently, sequence similarity between homologous viral proteins disappears quickly, limiting usability of traditional sequence-based phylogenetic methods in reconstruction relationships and evolutionary history among viruses. Protein structures, however, typically evolve more slowly than sequences, structural can still be evident, when no detected. Here, we used an automated comparison method, structure finder, for...
We developed a formulation of long double-stranded RNA (dsRNA) using interpolyelectrolyte complexes (IPECs) composed the biopolymers chitosan and alginate, in order to protect dsRNA from biotic abiotic factors. Our primary objectives were enhance stability against environmental nucleases and, secondarily, mitigate negative charge dsRNA, which may promote foliar uptake. approach relies on submicron particles with adjustable surface being either positive or negative. Following this approach,...
Virus-induced changes in cellular gene expression and host physiology have been studied extensively. Still, there are only a few analyses covering the entire viral replication cycle whole-host pool at resolution of single gene. Here we report Escherichia coli during bacteriophage PRD1 infection using microarray technology. Relative mRNA levels were systematically measured for over 99% open reading frames throughout cycle. Although drastic modifications could be detected individual genes,...
The activation of the interferon (IFN) system, which is triggered largely by recognition viral nucleic acids, one most important host defense reactions against infections. Although influenza A and B viruses, both have segmented negative-strand RNA genomes, share major structural similarities, they evolutionarily diverged, with total genetic incompatibility. Here we compare antiviral-inducing mechanisms during infections type viruses in human dendritic cells. We observed that IFN responses...
Recognition of viral genetic material takes place via several different receptor systems, such as retinoic acid-inducible gene I-like receptors and TLRs 3, 7, 8, 9. At present, systematic comparison the ability types RNAs to induce innate immune responses in human cells has been limited. In this study, we generated bacteriophage 6 influenza A virus-specific ssRNA dsRNA molecules ranging from 58 2956 nt. monocyte-derived dendritic (moDCs), short dsRNAs efficiently upregulated expression IFN...