A5071009771- Insect and Pesticide Research
- Plant and animal studies
- Insect and Arachnid Ecology and Behavior
- Insect-Plant Interactions and Control
- Plant Pathogens and Fungal Diseases
- Peanut Plant Research Studies
- Plant-Microbe Interactions and Immunity
- Plant Virus Research Studies
- Stress Responses and Cortisol
- Entomopathogenic Microorganisms in Pest Control
- Insect Pest Control Strategies
- Allelopathy and phytotoxic interactions
- Agronomic Practices and Intercropping Systems
- Agricultural pest management studies
- Plant Parasitism and Resistance
- Bee Products Chemical Analysis
- Plant and Fungal Interactions Research
- Plant Pathogenic Bacteria Studies
- Mycorrhizal Fungi and Plant Interactions
- Plant and soil sciences
- Nematode management and characterization studies
- Botanical Research and Applications
- Tryptophan and brain disorders
- Fungal Biology and Applications
- Essential Oils and Antimicrobial Activity
University of Concepción
2015-2024
Centro de Investigaciones Biológicas Margarita Salas
2003-2014
University of Zulia
2012
Consejo Superior de Investigaciones Científicas
2008
Duke Medical Center
1991-1993
Emory University
1993
Duke University Hospital
1992
Double-stranded RNA (dsRNA) is a potent initiator of gene silencing in diverse group organisms that includes plants, Caenorhabditis elegans, Drosophila and mammals. We have previously shown patented mechanical inoculation vitro-transcribed dsRNA derived from viral sequences specifically prevents virus infection plants. The approach required the vitro synthesis large amounts involving high cost considerable labour. developed an vivo expression system to produce virus-derived dsRNAs bacteria,...
Food losses, defined as a reduction in the quantity and quality of food during production storage, impact safety security. Losses caused by plant pathogens are among most significant. Chemical pesticides have been extensively used to prevent microbial diseases. Their toxicity reduced efficacy, however, encouraged investigators develop alternatives. Alternatives based on biopesticides tend be safer more environmentally benign than conventional pesticides. In recent years, formulations...
Considerable evidence indicates that corticotropin-releasing factor (CRF) is responsible for integrating not only the endocrine, but autonomic and behavioral responses of an organism to stress. We have investigated time course action anxiolytic triazolobenzodiazepine, alprazolam, on activity hypothalamic-pituitary-adrenal (HPA) axis CRF neurons after acute chronic administration. In addition, because many signs symptoms observed in animals humans abrupt discontinuation benzodiazepines...
SUMMARY A comparative analysis of the synergistic interaction between PVX and either PVY or TEV potyviruses was performed in Nicotiana benthamiana N. tabacum plants. In each PVX/potyvirus combination, doubly infected plants developed much more severe symptoms than singly ones. However, while accumulation increased compared with plants, did not vary drastically respect to single These findings suggest that relationship viral titre enhancement synergism infections is host dependent. Since...
The gut parasite Nosema ceranae is one of the most prevalent honey bee microsporidia worldwide and its chemical management in American countries mainly based on antibiotic fumagillin. However, it has recently been reported that this drug negative side effects bees; therefore, non-harmful alternative treatments must be found. methanolic extracts (2, 4, 8 16%) from Chilean native plant leaves (Aristotelia chilensis, Ugni molinae, Gevuina avellana) propolis (Biobío (BB) Los Ríos (LR) regions)...
Lotmaria passim currently appears to be the predominant trypanosome in honey bees worldwide. Although, specific effects of L. by single or mixed with other gut parasites such as Nosema ceranae on bees' health is still unclear. We consequently measured survival, parasite loads, expression antimicrobial peptides (AMPs) and vitellogenin gene. Thus, (1) naturally infected passim, (2) healthy inoculated ceranae, (3) N. (4) (control) were maintained under controlled conditions. Honey infections...
One hundred twenty-five yeast strains isolated from table grapes and apples were evaluated for the control Botrytis cinerea of in vitro vivo. Ten selected their ability to inhibit mycelial growth B. vitro. In vivo assays, these yeasts tested at 20 °C on ‘Thompson Seedless’ berries 7 days; only three (m11, me99 ca80) because they significantly reduced incidence gray mold. These then different concentrations (1 × 107, 1 108 109 cells mL−1) grape °C. The m11, ca80 11.9, 26.1 32.1%,...
Lentil is cultivated in Chilean Mediterranean drylands, areas with soils that are nutrient depleted and eroded. Inoculation of lentil rhizobia co-inoculation growth promoting rhizobacteria would allow higher biomass an opportunity for early nodulation increased nitrogen fixation. The objective this research was to select rhizosferic bacteria (PGPR) from lentils evaluate their effect on rhizobia. Sixty three isolates where obtained nine the mediterranean area. These were fingerprinted through...
Insect behavior is coordinated mainly by smell through the diverse odor-binding proteins (OBP) that allow them to identify and recognize their environment. Sensory information collected then analyzed interpreted in brain, allowing for correct insect functioning. The of honeybees (Apis mellifera L.) can be affected different pathogens, such as deformed wing virus (DWV). In particular, DWV variant A (DWV-A) capable altering olfactory sensitivity reducing gene expression OBPs, including those...
Click to increase image sizeClick decrease sizeKeywords: honey bee virusesNosema ceranaehoney mortalityblack queen cell virusdeformed wing virussacbrood virus
Different episodes of mortalities honey bee (Apis mellifera L.) colonies have been associated with the presence pathogens.Since Biobío Region has among highest number apiaries in Chile, aim present study was to identify viruses affecting bees, evaluate their relation other pathogens, and conduct a phylogenetic analysis.Pupae adult bees were collected from 60 Apis L. over 2 yr.RNA detected by reverse transcription PCR (RT-PCR), Acarapis woodi, Nosema spp., Varroa destructor via PCR.Three...
The honeybee (Apis mellifera L.) is an important pollinator worldwide. Beekeepers have experienced increasing loss of colonies in recent years and pathologies including viruses intestinal parasites such as Nosema ceranae are among those held responsible for this decline. aim study was to determine the prevalence distribution, using real time PCR method, six Deformed Wing Virus (DWV), Black Queen Cell (BQCV), Sacbrood (SBV), Acute Bee Paralysis (ABPV), Chronic (CBPV) Israeli (IAPV) addition...
Aegorhinus superciliosus is an important pest on blueberry (Vaccinium corymbosum L.) and other fruit trees. The use of entomopathogenic fungi as Metarhizium spp. has been evaluated for the control this insect, but variability observed among different strains. aim study was to characterize six promising strains A. superciliosus. studied were QuM173c, Qu-M363, Qu-M171a, Qu-M156a, Qu-M421, Qu-M430, all which belonged Chilean Collection Microbial Genetic Resources (ChCMGR) Instituto de...
Specific post-transcriptional gene silencing (PTGS) of target genes can be induced in a variety organisms by providing homologous double-stranded RNA (dsRNA) molecules. In plants, PTGS is part defense mechanism against virus infection. We have previously shown and patented that direct delivery to nontransgenic plants dsRNA derived from viral sequences specifically interfere with Here, we show transient expression constructs encoding hairpin rapidly replicating plant tobamovirus also...