A5106014401- Plant and Fungal Interactions Research
- Plant-Microbe Interactions and Immunity
- Insect Resistance and Genetics
- Plant Virus Research Studies
- Plant Pathogens and Fungal Diseases
- RNA Interference and Gene Delivery
- Silk-based biomaterials and applications
Hochschule Bielefeld
2024-2025
In vertebrates and plants, dsRNA plays crucial roles as PAMP a mediator of RNAi. How higher fungi respond to is not known. We demonstrate that Magnaporthe oryzae (Mo), globally significant crop pathogen, internalizes across broad size range 21 about 3000 bp. Incubation fungal conidia with 10 ng/µL dsRNA, regardless or sequence, induced aberrant germ tube elongation, revealing strong sequence-unspecific effect in this fungus. Accordingly, the synthetic analogue poly(I:C) various sizes...
We developed a formulation of long double-stranded RNA (dsRNA) using interpolyelectrolyte complexes (IPECs) composed the biopolymers chitosan and alginate, in order to protect dsRNA from biotic abiotic factors. Our primary objectives were enhance stability against environmental nucleases and, secondarily, mitigate negative charge dsRNA, which may promote foliar uptake. approach relies on submicron particles with adjustable surface being either positive or negative. Following this approach,...
<title>Abstract</title> In vertebrates and plants, double-stranded (ds)RNA plays crucial roles as a pathogen-associated molecular pattern (PAMP) mediator of RNA interference (RNAi). However, our understanding fungal responses to dsRNA remains limited. Here, we demonstrate that <italic>Magnaporthe oryzae</italic> (<italic>Mo</italic>), globally significant crop pathogen, actively internalizes exogenous across broad size range 21 about 3000 bp. Treatment <italic>Mo</italic> conidia with dsRNA,...