A5060920616- Bacteriophages and microbial interactions
- Evolution and Genetic Dynamics
- Antibiotic Resistance in Bacteria
- Microbial Community Ecology and Physiology
- Marine and coastal plant biology
- Genomics and Phylogenetic Studies
- Antibiotics Pharmacokinetics and Efficacy
- Protist diversity and phylogeny
- Gut microbiota and health
- Antimicrobial Resistance in Staphylococcus
- Plant Pathogens and Fungal Diseases
- Bacterial Genetics and Biotechnology
University of Jyväskylä
2020-2023
Abstract Bacteria often acquire resistance against antibiotics through the transfer of conjugative plasmids. Hence, it is vital to develop strategies mitigate dispersal antimicrobial (AMR). CRISPR-based tools offer a sequence-specific solution diminish and restrict dissemination genes among bacteria. CRICON (CRISPR via conjugation) an CRISPR tool that has been shown efficiently reduce multi-resistance when targeting ESBL (Extended Spectrum Beta-Lactamase) harboring However, conjugatively...
The rapid developments in the next-generation sequencing methods recent years have provided a wealth of information on community structures and functions endophytic bacteria. However, assembly processes these communities different plant tissues are still currently poorly understood, especially wild plants natural settings. aim this study was to compare composition bacterial leaves roots arcto-alpine pioneer Oxyria digyna, investigate, how tissue (leaf or root) origin affect assembly. To...
Antibiotic resistance genes of important Gram-negative bacterial pathogens are residing in mobile genetic elements such as conjugative plasmids. These rapidly disperse between cells when antibiotics present and hence our continuous use antimicrobials selects for that often harbor multiple genes. Plasmid-dependent (or male-specific or, some cases, pilus-dependent) bacteriophages viruses infect specifically bacteria carry certain The introduction these specialized phages into a...
Conjugative plasmids can confer antimicrobial resistance (AMR) to their host bacterium. The disperse even between distantly related species, rescuing the from otherwise detrimental effects of antibiotics. Little is known about role these in spread AMR during antibiotic treatment. One unstudied question whether past evolutionary history a plasmid particular species creates specificity its rescue potential or if interspecific coevolution improve rescues. To study this, we coevolved RP4 under...
Plasmids are extrachromosomal genetic elements, some of which disperse horizontally between different strains and species bacteria. They a major factor in the dissemination virulence factors antibiotic resistance. Understanding ecology plasmids has notable anthropocentric value, therefore, interactions bacterial hosts individual have been studied detail. However, systems often carry multiple genetically distinct plasmids, but dynamics within these multiplasmid communities remained unstudied....
Beta-lactams are commonly used antibiotics that prevent cell-wall biosynthesis. Beta-lactam sensitive bacteria can acquire conjugative resistance elements and hence become resistant even after being exposed to lethal (above minimum inhibitory) antibiotic concentrations. Here we show neither the length of exposure (1 16 h) nor beta-lactam type (penam or cephem) have a major impact on rescue bacteria. We demonstrate an evolutionary occur between different clinically relevant bacterial species...
Abstract Plasmids are genetic elements that disperse horizontally between different strains and species of bacteria a major factor in the dissemination virulence factors antibiotic resistance. Understanding ecology plasmids has notable anthropocentric value therefore interactions bacterial hosts individual have been studied detail. However, systems often carry multiple genetically distinct plasmids, but dynamics these multiplasmid “clashes” remained unstudied. Here, we set to investigate...