A5066573213- Developmental Biology and Gene Regulation
- Congenital heart defects research
- Pluripotent Stem Cells Research
- Animal Genetics and Reproduction
- Epigenetics and DNA Methylation
- Genetic and Kidney Cyst Diseases
- Renal and related cancers
- Genetic Syndromes and Imprinting
- Genomics and Chromatin Dynamics
- CRISPR and Genetic Engineering
- Spine and Intervertebral Disc Pathology
- Wnt/β-catenin signaling in development and cancer
- Genomic variations and chromosomal abnormalities
- Neurogenesis and neuroplasticity mechanisms
- Zebrafish Biomedical Research Applications
- RNA Research and Splicing
- TGF-β signaling in diseases
- Microtubule and mitosis dynamics
- Hedgehog Signaling Pathway Studies
- Kruppel-like factors research
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Genetics and Neurodevelopmental Disorders
- Cellular Mechanics and Interactions
- Angiogenesis and VEGF in Cancer
- Axon Guidance and Neuronal Signaling
Medizinische Hochschule Hannover
2015-2025
Institute of Molecular Biology
2002-2019
Max Planck Institute for Heart and Lung Research
2008
Kerckhoff Klinik
2007
Lunenfeld-Tanenbaum Research Institute
1992-2004
Mount Sinai Hospital
1989-2004
Max Planck Institute for Molecular Genetics
2004
Max Planck Institute for Biophysical Chemistry
2004
Max Planck Institute of Immunobiology and Epigenetics
2004
Jackson Laboratory
1995-2001
A strategy was devised for identifying regions of the mouse genome that are transcriptionally active in a temporally and spatially restricted manner during development. The approach is based on introduction into embryonic stem cells two types lacZ reporter constructs can be activated by flanking genomic sequences. Embryonic containing were used to produce chimaeric mice. Developmental regulation expression occurred at high frequency. Molecular cloning endogenous genes these potential...
ABSTRACT The Drosophila Delta (Dl) gene is essential for cell-cell communication regulating the determination of various cell fates during development. Dl encodes a transmembrane protein, which contains tandem arrays epidermal-growth-factor-like repeats in extracellular domain and directly interacts with Notch, another protein similar structural features, ligand-receptor-like manner. Similarly, interactions involving Delta-like Notch-like proteins are required fate determinations C. elegans....
This study demonstrates that blastocyst-derived embryonic stem cells (ES cells) can be used as a vehicle for transgenesis. The method is nearly efficient other methods, and the introduced neomycin phosphotransferase (neo) gene stably transmitted through several generations with no apparent loss in G418 resistance. An important factor contributing to efficiency of this process rigorous selection, before blastocyst injection, genetically transformed vitro developmental pluripotency. One...
The Notch pathway regulates cell fate determination in numerous developmental processes. Here we report that Notch2 acts non-redundantly to control the processes of nephron segmentation through an Rbp-J-dependent process. Notch1 and are detected early renal vesicle. Genetic analysis reveals only is required for differentiation proximal structures (podocytes convoluted tubules)despite presence activated nuclei putative progenitors. inability endogenous compensate deficiency may reflect...
ABSTRACT Somitic segmentation provides the framework on which segmental pattern of vertebrae, some muscles and peripheral nervous system is established. Recent evidence indicates that a molecular oscillator, ‘segmentation clock’, operates in presomitic mesoderm (PSM) to direct periodic expression c-hairy1 lunatic fringe (l-fng). Here, we report identification characterisation second avian hairy-related gene, c-hairy2, also cycles PSM whose sequence closely related mammalian HES1 downstream...
The mammalian auditory sensory epithelium, the organ of Corti, contains hair cells and nonsensory supporting arranged in a highly patterned mosaic. Notch-mediated lateral inhibition is proposed mechanism for creating this Previous work has shown that mice lacking Notch ligand JAG2 differentiate supernumerary cochlea,consistent with inhibitory model. However, it was not clear why only relatively modest increases cell production were observed Jag2 mutant mice. Here, we show another ligand,...
In vertebrates, skeletal myogenesis is initiated by the generation of myoblasts followed their differentiation to myocytes and formation myofibers. The determination are controlled muscle regulatory factors that activated at specific stages during myogenesis. During late embryonic fetal a distinct population resident proliferating progenitor cells major source myogenic cells. How regulated not clear. We show in mouse embryos Notch ligand Delta1 (Dll1) controls both early maintenance Early...
Notch signaling in the presomitic mesoderm (psm) is critical for somite formation and patterning. Here, we show that WNT signals regulate transcription of ligand Dll1 tailbud psm. LEF/TCF factors cooperate with TBX6 to activate from promoter vitro. Mutating either T or sites abolishes reporter gene expression vitro as well tail bud psm transgenic embryos. Our results indicate activity, synergy TBX6, regulates thereby controls formation,
The Notch ligands Dll1 and Dll3 are coexpressed in the presomitic mesoderm of mouse embryos. Despite their coexpression, mutations cause strikingly different defects. To determine if there is any functional equivalence, we replaced with mice. does not compensate for Dll1; DLL1 activates Drosophila wing discs, but DLL3 not. We do observe evidence antagonism between DLL3, or repression activity mice Drosophila. In vitro analyses show that differences various domains individually contribute to...
Cytokinesis terminates mitosis, resulting in separation of the two sister cells. Septins, a conserved family GTP-binding cytoskeletal proteins, are an absolute requirement for cytokinesis budding yeast. We demonstrate that septin-dependence mammalian differs greatly between cell types: genetic loss pivotal septin subunit SEPT7 vivo reveals septins indispensable fibroblasts, but expendable cells hematopoietic system. SEPT7-deficient mouse embryos fail to gastrulate, and septin-deficient...
In eutherian mammals, implantation and establishment of the chorioallantoic placenta are essential for embryo development survival. As a maternal response to implantation, uterine stromal cells proliferate, differentiate, generate decidua, which encapsulates conceptus forms part placenta. Little is known about decidual functions molecular interactions that regulate its maintenance. Here we show receptor cytokine interleukin-11 (IL-11Ralpha) required specifically normal decidua. Females...