A5059913180- Viral Infectious Diseases and Gene Expression in Insects
- CRISPR and Genetic Engineering
- Virus-based gene therapy research
- Animal Genetics and Reproduction
- 3D Printing in Biomedical Research
- Tissue Engineering and Regenerative Medicine
- Monoclonal and Polyclonal Antibodies Research
- Pluripotent Stem Cells Research
- RNA Interference and Gene Delivery
- Muscle Physiology and Disorders
- Liver physiology and pathology
- Electrospun Nanofibers in Biomedical Applications
- Helicobacter pylori-related gastroenterology studies
- Cellular Mechanics and Interactions
- Transgenic Plants and Applications
- Nanoparticle-Based Drug Delivery
- Protein purification and stability
- Surgical Simulation and Training
- Cancer, Hypoxia, and Metabolism
- Cancer Diagnosis and Treatment
- Neuroscience and Neural Engineering
- Allergic Rhinitis and Sensitization
- Microfluidic and Bio-sensing Technologies
- Pancreatic function and diabetes
- Spaceflight effects on biology
Kyushu University
2015-2024
KM Biologics (Japan)
2022
ECSI Fibrotools (United States)
2010-2015
Nagoya University
1988-2013
Toyota Motor Corporation (Switzerland)
2012
Hosei University
2006
University of Tsukuba
2002
Osaka Medical and Pharmaceutical University
1970-1972
Aichi Cancer Center
1966
Electrical impulses are necessary for proper in vivo skeletal muscle development. To fabricate functional tissues vitro, recapitulation of the niche, including physical stimuli, is crucial. Here, we report a technique to engineer vitro by electrical pulse stimulation (EPS). Electrically excitable tissue-engineered constructs were stimulated with continuous pulses 0.3 V/mm amplitude, 4 ms width, and 1 Hz frequency, resulting 4.5-fold increase force at day 14. In myogenic differentiation...
Gene knock-in techniques have rapidly evolved in recent years, along with the development and maturation of genome editing technology using programmable nucleases. We recently reported a novel strategy for microhomology-mediated end-joining-dependent integration donor DNA by TALEN or CRISPR/Cas9 optimized targeting vectors, named PITCh (Precise Integration into Target Chromosome) vectors. Here we describe vector-mediated long gene cassettes, including single-chain Fv-Fc (scFv-Fc) gene,...
Skeletal muscle tissue engineering is currently applied in a variety of research fields, including regenerative medicine, drug screening, and bioactuator development, all which require the fabrication biomimic functional skeletal tissues. In present study, magnetite cationic liposomes were used to magnetically label C2C12 myoblast cells for construction three-dimensional artificial tissues by an magnetic force. functions, such as biochemical contractile properties, evaluated constructs....
Genomics-based safety and quality evaluation studies are advancing the bioindustry by enhancing various aspects, including viral safety, host cell protein (HCP) control, product heterogeneity cellular process reproducibility. High-throughput instruments genome-scale databases essential in genomics, with reference genome sequence being most critical database. The completeness accuracy of these sequences depend on DNA quality, sequencing instruments, read coverage, assembly strategies....
The Cre-loxP system is frequently used for site-specific recombination in animal cells. equilibrium and specificity of the reaction can be controlled using mutated loxPs. In present study, we designed an accumulative gene integration Cre recombinase loxPs which Cre-mediated cassette exchange infinitely repeatable target into loxP sites. To evaluate feasibility usefulness this system, a series reactions were repeated confirmed vitro protein plasmids. Accumulative was also performed on genome...
Abstract Skeletal muscle tissue engineering holds great promise for pharmacological studies. Herein, we demonstrated an in vitro drug testing system using tissue-engineered skeletal constructs. In response to epigenetic drugs, myotube differentiation of C2C12 myoblast cells was promoted two-dimensional cell cultures, but the levels contractile force generation constructs prepared by three-dimensional cultures were not correlated with cultures. contrast, sarcomere formation and activity...
Here we applied a magnetic force-based tissue engineering technique to cardiac fabrication. A mixture of extracellular matrix precursor and cardiomyocytes labeled with nanoparticles was added into well containing central polycarbonate cylinder. With the use magnet, cells were attracted bottom allowed form cell layer. During cultivation, layer shrank towards cylinder, leading formation ring-shaped that possessed multilayered structure contractile properties. These results indicate fabrication...
Antigen-specific immunotherapy is the only curative approach for treatment of allergic diseases such as Japanese cedar pollinosis. Immunotherapy using a T cell epitope vaccine in combination with adjuvant R848 particular interest safe and effective to treat diseases. Herein, we propose simple easy handle administration method original solid-in-oil (S/O) nanodispersion system that permeates through skin. The S/O composed nanoparticles hydrophilic molecules surrounded hydrophobic surfactants...
Tissue-engineered skeletal muscle constructs should be designed to generate contractile force with directional movement. Because electrical impulses from a somatic nervous system are crucial for in vivo development, pulse stimulation (EPS) culture as an artificial exercise is essential fabricate functional tissues vitro. To further improve functions, the activation of cell-signaling pathways myogenic growth factors, such insulin-like factor (IGF)-I, also important. tissue-engineered maintain...