Serratia marcescens is an important opportunistic pathogen in hospitals, where quaternary ammonium compounds are often used for disinfection. The aim of this study to elucidate the effect a biocide on emergence biocide- and antibiotic-resistant mutants characterize molecular mechanism resistance marcescens. A compound-resistant strain, CRes01, was selected by exposing wild-type strain S. cetylpyridinium chloride. CRes01 cells exhibited 2- 16-fold more than biocides antibiotics, including...

Single crystals of alkoxy-functionalized hydroxamate/zinc metal–organic frameworks (MOFs) were obtained by fixating the hydroxamate moiety via intramolecular hydrogen bonding. The resulting MOF structures depend on steric demand alkoxy groups, whereby incorporation bulky isopropyl groups affords porous MOFs. topological isopropyl-substituted MOFs could be controlled adding acid.

The gemini quaternary salt (gemini-QUAT) containing two pyridinium residues per molecule, 3,3'-(2,7-dioxaoctane) bis (1-decylpyridinium bromide) (3DOBP-4,10), exerted fungicidal activity against Saccharomyces cerevisiae accompanied by respiration inhibition and the cytoplasmic material leakage of ATP, magnesium, potassium ions. We previously found that gemini-QUAT bacterioclastic action Escherichia coli causing rapid abundant turbid materials from cells. In addition, first stage was...

Abstract The NfxC‐type mutant of Pseudomonas aeruginosa produces the MexEF‐OprN efflux pump and down‐regulates expression quorum‐sensing‐dependent MexAB‐OprM production virulence factors in presence an active transcriptional regulator, MexT. Consequently, these cells are resistant to chloramphenicol hypersusceptible β‐lactam antibiotics. An upper negative MexS, has been assumed inactivate MexT wild‐type strains, hence shutting down pump. This observation was, however, reported only one...

Abstract We report the mixed-ligand synthesis of a novel hydroxamate/zinc metal-organic framework (MOF) with dibenzothiophene scaffold. The reaction dibenzothiophene-3,7-dicarbohydroxamic acid, isonicotinic and zinc nitrate under solvothermal conditions afforded porous MOF. structure gas-adsorption properties toward N2, H2, CO2, CH4 MOF were investigated.

Abstract The low energy of UV-A (315–400 nm) is insufficient for disinfection. To improve disinfection technology, we evaluated the effect ferulic acid (FA) addition on by light-emitting diode (LED) (350-385 against various food spoilers and pathogens (seven bacteria four fungi species). Photoantimicrobial assays were performed at FA concentrations below MIC. MIC isomerized FA, consisting 93% cis-form 7% trans-form, was very similar to that commercially available (trans-form). Irradiation...

Enhanced photocatalytic activity of rutile-based TiO2 materials under general lighting is practically desired. O2 plasma-assisted annealing (PAA) effects on Pt-doped rutile nanoparticles were clarified along with its visible-light-driven enhancement. The PAA-treated samples mainly analyzed using optical spectroscopy and x-ray photoelectron (XPS). was assessed by decomposing methylene blue dye inactivating Bacillus subtilis lighting. PAA treatment changed the O 1s, Ti 2p, Pt 4f spectra XPS...

We synthesized gemini quaternary ammonium compounds (gemini QACs) having two thiazolium moieties in a molecule, 5,5'-[2,2'-(alpha,omega-polymethylnedicarbonyldioxy)diethyl]bis(3-alkyl-4-methylthiazolium iodide) (5DEBT-m,n), on which the carbon number of methylene chain linking thiazoles (m) is 2, 6 or 8 and that alkyl group (n) 8, 10, 12, 14 16. 5,5'-[2,2'-(p-Phenylenedicarbonyldioxy)diethyl]bis(3-alkyl-4-methylthiazolium bromide) (5DEBT-P,n) was then synthesized, composed p-phenylene as...

We previously found that the gemini quaternary salt (gemini-QUAT) containing two pyridinium residues per molecule, 3,3'- (2,7-dioxaoctane) bis (1-decylpyridinium bromide) (3DOBP-4,10) , exerted fungicidal activity against Saccharomyces cerevisiae and caused respiration inhibition cytoplasmic leakage of ATP, magnesium, potassium ions. Here, we investigated how gemini-QUAT, 3DOBP-4,10, exerts more powerful antimicrobial than mono-QUAT N-cetylpyridinium chloride (CPC) examined association...

A single-step immunoassay device for ultrafast measurement of proteins within 40 seconds, fabricated by using a square glass capillary, antibody–graphene oxide conjugate and fluorescently labelled antibody, was developed.

Ultraviolet-A (UV-A)-mediated bactericidal activity was enhanced by combined treatment with trans-ferulic acid (trans-FA, compound 1) or its derivatives. Derivative compounds 4 and 10 contain a phenyl group an l-tyrosine HCl tert-butyl ester, respectively, linked to the carboxyl of trans-FA. Of three compounds, exhibited highest synergistic in photobactericidal assay based on treating Escherichia coli derivative UV-A irradiation (wavelength 350-385 nm). Inactivation viable cells at 4.9 J...

5a-h, a series of (5-substituted-2-methyl-1,3-thiazole-4-yl) acetic acids as heterocyclic acid derivatives, was designed and synthesized from ethyl acetoacetate. The compounds were screened for their antimicrobial activities against bacterial fungal strains, characteristics investigated by assays under various temperature pH conditions. Cytotoxicity evaluated with the use sheep erythrocytes human neonate dermal fibroblasts. Similarly, agents such lauric 6 parabens 7a-b, which are used...

Ultraviolet-A (UV-A) can damage microbes by generating reactive oxygen species (ROS), singlet oxygen, superoxides, hydrogen peroxide and hydroxyl radicals. These readily react with lipids, proteins, DNA other constituents of cells, leading to oxidative deterioration the eventual death microbe. However, ability these also harms viability mammalian cells such as fibroblasts keratinocytes, they cause both acute chronic damage, photo-aging, photo-carcinogenesis. This study describes a UV-A...

A UiO-67-type Zr-based metal–organic framework (MOF) with dibenzothiophene linkers, UiO-67-S, was synthesized using a solvothermal method. The crystal structure of UiO-67-S exhibits the same topological as UiO-67 and its derivatives. shows high thermal stability excellent permanent porosity. Moreover, hydrogen storage indicates that thiophene moiety is suitable for enhancing ability MOFs.

A facile carbon‐doping process is proposed to enhance the photocatalytic activity of anatase/rutile‐mixed phase TiO 2 nanoparticles using polyethylene glycol (PEG). The ‐PEG composite loaded into a boat and covered tightly with Al foil increase pressure inside that during annealing. annealed for 1 h at different temperatures PEG ratios. annealing 30% 300 °C enhances decomposition organic pollutants bacterial inactivation under 405 nm light compared without films. This causes 2.5–3% carbon...

Serratia marcescens gained resistance to both biocides and antibiotics on expressing the SdeAB efflux pump, following exposure increasingly higher concentrations of a biocide (H. Maseda et al., Antimicrob. Agents Chemother. 53:5230-5235, 2009). To reveal regulatory mechanism sdeAB expression, wild-type cells were subjected transposon-mediated random mutagenesis, mutant with antibiotic resistance, which mimicked phenotype previous biocide-resistant cells, was obtained. The transposon element...