A5091192903- Cellular transport and secretion
- Photosynthetic Processes and Mechanisms
- RNA Interference and Gene Delivery
- Plant tissue culture and regeneration
- CAR-T cell therapy research
- Virus-based gene therapy research
- Retinal Development and Disorders
- Plant Molecular Biology Research
- Plant Stress Responses and Tolerance
- Nutrition and Health in Aging
- Japanese History and Culture
- HIV Research and Treatment
- Advanced Fluorescence Microscopy Techniques
- Plant Reproductive Biology
- Immune Cell Function and Interaction
- Photoreceptor and optogenetics research
- Plant Disease Management Techniques
- Advanced Electron Microscopy Techniques and Applications
- Endoplasmic Reticulum Stress and Disease
- Stroke Rehabilitation and Recovery
- Lysosomal Storage Disorders Research
- Photovoltaic Systems and Sustainability
- Potassium and Related Disorders
- Magnetic and Electromagnetic Effects
- Autophagy in Disease and Therapy
RIKEN Center for Sustainable Resource Science
2009-2025
Kurashiki Central Hospital
2023
National Institute for Materials Science
2023
Tokyo University of Agriculture
2019
Toho University
2018
Takara (Japan)
2008-2012
Nara Institute of Science and Technology
2008
Kyoto University
2008
Institute of Agrobiological Sciences
2008
Research Institute for Sustainable Humanosphere
2008
Abstract Nicotine is a major alkaloid accumulating in the vacuole of tobacco (Nicotiana tabacum), but transporters involved vacuolar sequestration are not known. We here report that genes (NtMATE1 and NtMATE2) encoding multidrug toxic compound extrusion (MATE) family coordinately regulated with structural for nicotine biosynthesis root, respect to spatial expression patterns, regulation by NIC regulatory loci, induction methyl jasmonate. Subcellular fractionation, immunogold electron...
Secretory proteins and extracellular glycans are transported to the space during cell growth. These materials carried in secretory vesicles generated at trans-Golgi network (TGN). Analysis of mammalian post-Golgi pathway demonstrated movement separated cell. Using carrier membrane protein 2 (SCAMP2) as a marker for tobacco (Nicotiana tabacum) BY-2 model cell, we characterized transport machinery plant cells. A combination analyses, including electron microscopy quick-frozen cells...
Abstract The trans -Golgi network (TGN) has been known as a key platform to sort and transport proteins their final destinations in post-Golgi membrane trafficking. However, how the TGN sorts with different destinies still remains elusive. Here, we examined 3D localization 4D dynamics of TGN-localized Arabidopsis thaliana that are involved either secretory or vacuolar trafficking from TGN, by multicolor high-speed high-resolution spinning-disk confocal microscopy approach developed. We...
When a fusion protein of cytochrome b5 (Cyt b5) and the red fluorescent (RFP) are expressed in tobacco BY-2 cells, forms intracellular aggregates that emit fluorescence. such cells grown to stationary phase or incubated nutrient limited medium, RFP fluorescence can be detected vacuolar lumen. We investigated this transport mechanism using limited-nitrogen model. E-64 3-methyladenine, which inhibit autophagic processes, blocked signal vacuole. next traced process YFP fused with Atg8 homologue...
Abstract The thylakoid membrane (TM) serves as the scaffold for oxygen-evolving photosynthesis, hosting protein complexes responsible light reactions and ATP synthesis. Vesicle Inducing Protein in Plastid 1 (VIPP1), a key TM remodeling, has been recognized essential homeostasis. In vitro studies of cyanobacterial VIPP1 demonstrated its ability to form large homo-oligomers (> 2 MDa) manifesting ring-like or filament-like assemblies associated with membranes. Similarly, Chlamydomonas...
Direct delivery of proteins into plants represents a promising alternative to conventional gene for probing and modulating cellular functions without the risk random integration transgenes host genome. This remains challenging, however, because lack protein tool applicable diverse plant species limited information about entry mechanisms exogenous in cells. Here, we present synthetic multidomain peptide (named dTat-Sar-EED4) cytosolic various via simple peptide-protein coincubation....
Targeted delivery of genes to specific plant organelles is a key challenge for fundamental science, bioengineering, and agronomic applications. Nanoscale carriers have attracted interest as promising tool organelle-targeted DNA in plants. However, nanocarrier-mediated plants severely hampered by the barrier cell wall, resulting insufficient efficiency. Herein, we propose unique strategy that synergistically combines wall-loosening zwitterionic liquid (ZIL) with peptide-displaying micelle...
Adoptive immunotherapy using TCR gene-modified T-lymphocytes is an attractive strategy for targeting malignancies. However, mispairings between endogenous and introduced chains are a major concern, as they may induce mixed TCRs with unknown specificities reduce the expression of therapeutic TCRs. To overcome these problems, we have recently established novel retroviral siTCR vector encoding small-interfering RNAs (siRNAs) to knockdown genes efficient In this study, improve efficacy vectors,...
Rapid growth of plant cells by cell division and expansion requires an endomembrane trafficking system. The compartments, such as the Golgi stacks, endosome vesicles, are important in synthesis wall materials during elongation. However, changes morphology, distribution number these compartments different stages proliferation differentiation have not yet been clarified. In this study, we examined at ultrastructural level tobacco Bright yellow 2 (BY-2) log stationary phases growth. We analyzed...
Programmed cell death (PCD) in lateral root caps (LRCs) is crucial for maintaining cap functionality. Endoplasmic reticulum (ER) bodies play important roles plant immunity and PCD. However, the distribution of ER their communication with vacuoles LRC remain elusive. In this study, we investigated ultrastructure cells wild-type transgenic Arabidopsis lines using an auto-acquisition transmission electron microscope (TEM) system high-pressure freezing. Gigapixel-scale high-resolution TEM...
NK cells play important roles in immune surveillance against malignancy and virus-infected cells. cell functions are affected patients infected with HIV-1; however, whether there is direct interaction between HIV-1 remains controversial. In this study the expression of CD4, an receptor for HIV-1, was up-regulated on co-cultured cell-selective stimulating line, HFWT, rIL-2. Although level CD4 lower than CD4+ T cells, co-receptor CCR5 clearly expressed higher levels HLA-DR CD25 CD4- suggesting...
Correlative array tomography, combining light and electron microscopy via serial sections, plays a crucial role in the three-dimensional ultrastructural visualization molecular distribution analysis biological structures. To address challenges of aligning fluorescence images sections irregularly shaped specimens, we developed diamond notch knife, new tool for puncturing holes using needle. The needle featured triangular right-angled tip, enabling drilling deep upon insertion into polished...
A comprehensive study of soluble
Abstract There is known to be a close relationship among anxiety, fatigue and stress. Here, we studied the anxiolytic‐like effects of essential oil from Thymus vulgaris ( EOT ), while focusing on with Male ICR mice inhaled (i.h.) for 90 min. Mice were placed under three different conditions; isolated, intraperitoneal (i.p.) administration saline or polyinosinic‐polycytidylic acid (poly I:C). did not show any significant in isolated mice. On other hand, brain model by poly I:C, showed...
Large scale T-cell expansion and efficient gene transduction are required for adoptive therapy. Based on our previous observations, human peripheral blood mononuclear cells (PBMCs) can be expanded efficiently while conserving a naïve phenotype by stimulating with both recombinant fibronectin fragment (CH-296) anti-CD3 monoclonal antibodies. In this article, we explored the possibility of using co-stimulation method to generate engineered T lentiviral vector. Human PBMCs were stimulated...
Live imaging of secretory cargoes is a powerful method for understanding the mechanisms membrane trafficking. Inducing synchronous release from an organelle key enhancing microscopic observation. We developed optical cargo-releasing method, 'retention using dark state LOV2' (RudLOV), which enables precise spatial, temporal, and quantity control during cargo release. A limited amount cargo-release RudLOV able to visualize cisternal-movement cargo-specific exit sites on Golgi/trans-Golgi...