A5002433760- Photosynthetic Processes and Mechanisms
- Light effects on plants
- Plant Molecular Biology Research
- RNA Interference and Gene Delivery
- Plant tissue culture and regeneration
- Photoreceptor and optogenetics research
- Advanced Fluorescence Microscopy Techniques
- Plant-Microbe Interactions and Immunity
- Plant Reproductive Biology
- Advanced biosensing and bioanalysis techniques
- Algal biology and biofuel production
- Marine and coastal plant biology
- Plant Virus Research Studies
- Bryophyte Studies and Records
- Transgenic Plants and Applications
- Biocrusts and Microbial Ecology
- Silk-based biomaterials and applications
- Plant and Biological Electrophysiology Studies
- Plant Stress Responses and Tolerance
- Plant nutrient uptake and metabolism
- RNA and protein synthesis mechanisms
- ATP Synthase and ATPases Research
- CRISPR and Genetic Engineering
- Plant Gene Expression Analysis
- Chromosomal and Genetic Variations
Utsunomiya University
2016-2025
RIKEN Center for Sustainable Resource Science
2011-2024
Tokyo University of Agriculture and Technology
2019-2023
Bioscience Research
2015-2023
Kyushu University
2009-2018
Bio-Medical Science (South Korea)
2017
Plant (United States)
2011
National Institute for Basic Biology
2007-2010
Purdue University West Lafayette
2010
Nara Institute of Science and Technology
2005-2009
Protein-protein interactions (PPIs) play crucial roles in various biological processes. Among biochemical, genetic, and imaging approaches that have been used for the study of PPIs, visualization PPIs living cells is key to understanding their cellular functions. The bimolecular fluorescence complementation (BiFC) assay represents one these tools direct cells. BiFC based on structural two nonfluorescent N- C-terminal fragments a fluorescent protein when they are fused pair interacting...
Significance Living organisms perceive temperature using thermosensory molecules. In this study, we found that phototropin, a blue-light photoreceptor, perceives via mechanism based on the photoactivated chromophore’s lifetime to induce appropriate chloroplast positioning in plants. Our findings reveal chromophore of phototropin directs optimize photosynthesis plants by ( i ) sensing blue light and ii temperature-dependent mechanism. Because many photoreceptors range contain phtoactivatable...
Quick and facile transient RNA interference (RNAi) is one of the most valuable plant biotechnologies for analysing gene functions. To establish a novel double-strand (dsRNA) delivery system plants, we developed an ionic complex synthetic dsRNA with carrier peptide in which cell-penetrating fused polycation sequence as carrier. The dsRNA-peptide 100-300 nm diameter positively charged. Infiltration into intact leaf cells Arabidopsis thaliana successfully induced rapid efficient down-regulation...
To develop a new easy and quick gene delivery system for any types of plants, we prepared ionic complexes plasmid DNA with designed peptide carriers, each which combined cell-penetrating (Bp100 or Tat(2)) polycation (nona-arginine copolymer histidine lysine). The present via the peptides demonstrated rapid efficient transient transfections into intact leaf cells Nicotiana benthamiana Arabidopsis thaliana without protoplast preparations. significantly higher transfection efficiency in...
Chloroplast, an organelle facilitating photosynthesis, exhibits strong autofluorescence, which is undesired background signal that restricts imaging experiments with exogenous fluorophore in plants. In this study, the autofluorescence was characterized planta under confocal laser microscopy, and it found time-gated technique completely eliminates autofluorescence. As a demonstration of technique, clearer fluorescent protein-tagged phototropin, blue-light photoreceptor localized at...
Available methods in plant genetic transformation are nuclear and plastid transformations because similar procedures have not yet been established for the mitochondria. The double membrane small size of organelle, addition to its large population cells, major obstacles mitochondrial transfection. Here we report intracellular delivery exogenous DNA localized mitochondria Arabidopsis thaliana using a combination mitochondria-targeting peptide cell-penetrating peptide. Low concentrations...
Genetic engineering of economically important traits in plants is an effective way to improve global welfare. However, introducing foreign DNA molecules into plant genomes create genetically engineered not only requires a lengthy testing period and high developmental costs but also well-accepted by the public due safety concerns about its effects on human animal health environment. Here, we present high-throughput nucleic acids delivery platform for using peptide nanocarriers applied leaf...
The delivery of genetic material into plants has been historically challenging due to the cell wall barrier, which blocks passage many biomolecules. Carbon nanotube-based emerged as a promising solution this problem and shown effectively deliver DNA RNA intact plants. Mitochondria are important targets their influence on agronomic traits, but organelle limited low efficiencies, restricting potential in engineering. This work describes use carbon nanotube-polymer hybrid modified with...
Selective gene delivery into organellar genomes (mitochondrial and plastid genomes) has been limited because of a lack appropriate platform technology, even though these organelles are essential for metabolite energy production. Techniques selective modification needed to functionally improve produce transplastomic/transmitochondrial plants. However, no method mitochondrial genome yet established multicellular organisms including Likewise, few plant species algae. In the present study, we...
In current plant biotechnology, the introduction of exogenous DNA encoding desired traits is most common approach used to modify plants. However, general transformation methods can cause random integration into genome. To avoid these events, alternative methods, such as a direct protein delivery system, are needed plant. Although there have been reports proteins cultured cells, currently no for intact plants, owing their hierarchical structures. Here, we demonstrate efficient...
Chloroplasts move toward weak light (accumulation response) and away from strong (avoidance response). The fast accurate movement of chloroplasts in response to ambient conditions is essential for efficient photosynthesis photodamage prevention chloroplasts. Here, we report that two Arabidopsis mutants, ak chloroplast under b lue 1 ( web1 ) web2 , are defective both the avoidance accumulation responses. Map-based cloning revealed genes encode coiled-coil proteins WEB2 identical plastid...
The liverwort Marchantia polymorpha L. is being developed as an emerging model plant, and several transformation techniques were recently reported. Examples are biolistic- Agrobacterium-mediated methods. Here, we report a simplified method for of sporelings, it termed Agar-utilized Transformation with Pouring Solutions (AgarTrap). procedure the AgarTrap was carried out by simply exchanging appropriate solutions in Petri dish, completed within week, successfully yielding sufficient numbers...
Liverworts are key species for studies of plant evolution, occupying a basal position among the land plants. Marchantia polymorpha has emerged as highly studied model liverwort, and many relevant techniques, including genetic transformation, have been established this species. Agrobacterium-mediated transformation is widely used in because its low cost. Recently, we developed simplified method transforming M. polymorpha, known AgarTrap (agar-utilized with pouring solutions). The procedure,...
Significance SNF1-related protein kinase 2s (SnRK2s) are central regulators in abscisic acid (ABA) signaling and stress responses plants. However, SnRK2 substrates largely unknown. Here, we identified Arabidopsis group C Raf-like kinases Raf36 Raf22 as substrates. Reverse genetic analyses showed that negatively regulate ABA signaling. Under optimal growth conditions, accumulates the cell phosphorylates to suppress responses. Conversely, under abiotic ABA-activated SnRK2s phosphorylate...
Abstract Organisms adapt to predictable environmental changes via a biological mechanism called priming. Phototropin (phot) is plant-specific blue light photoreceptor that mediates daily light-induced responses, such as chloroplast relocation, stomatal opening, and phototropism, optimize photosynthesis. Phot also functions thermosensor for relocation may sense temperature decreases at night, thereby modulating responses dawn; however, this hypothesis has not yet been fully explored. Here, we...
Organelles change their subcellular positions in response to various environmental conditions. Recently, we reported that cold treatments alter the intracellular position of chloroplasts and nuclei (cold positioning) fern Adiantum capillus-veneris; localized periclinal cell wall relocated anticlinal after treatments. To further understand organelle positioning under conditions, studied cold-induced relocation liverwort Marchantia polymorpha L. When sporelings gemmmalings were treated low...
Direct delivery of proteins into plants represents a promising alternative to conventional gene for probing and modulating cellular functions without the risk random integration transgenes host genome. This remains challenging, however, because lack protein tool applicable diverse plant species limited information about entry mechanisms exogenous in cells. Here, we present synthetic multidomain peptide (named dTat-Sar-EED4) cytosolic various via simple peptide-protein coincubation....
Simple and efficient cell-penetrating peptide-based multiple DNA, RNA protein codelivery platforms to plant cells have been developed.
Abstract Wound-induced protein kinase (WIPK) is a tobacco (Nicotiana tabacum) mitogen-activated known to play an essential role in defense against wounding and pathogens, although its downstream targets have yet be clarified. This study identified gene encoding of 648 amino acids, which directly interacts with WIPK, designated as N. tabacum WIPK-interacting factor (NtWIF). The N-terminal region approximately 250 acids showed high similarity the plant-specific DNA binding domain, B3, but no...
Abstract Non-viral gene carriers have been extensively investigated as alternatives to viral vectors for delivery systems into animal and plant cells. A non-viral carrier containing a cell-penetrating peptide cationic sequence was previously developed use in intact plants cells; however, the permeation pathway of cells is yet be elucidated, which would facilitate improvement efficiency. Here, we identified vacuum/compression infiltration-mediated tissues using complex plasmid DNA...