A5041739252- Plant Molecular Biology Research
- Plant Stress Responses and Tolerance
- Plant-Microbe Interactions and Immunity
- Plant nutrient uptake and metabolism
- Photosynthetic Processes and Mechanisms
- Plant Gene Expression Analysis
- Plant responses to water stress
- Plant Disease Resistance and Genetics
Tokyo University of Agriculture and Technology
2020-2024
Significance SNF1-related protein kinase 2s (SnRK2s) are central regulators in abscisic acid (ABA) signaling and stress responses plants. However, SnRK2 substrates largely unknown. Here, we identified Arabidopsis group C Raf-like kinases Raf36 Raf22 as substrates. Reverse genetic analyses showed that negatively regulate ABA signaling. Under optimal growth conditions, accumulates the cell phosphorylates to suppress responses. Conversely, under abiotic ABA-activated SnRK2s phosphorylate...
SUMMARY Abscisic acid (ABA) is a phytohormone that promotes leaf senescence in response to environmental stress. We previously identified methyl CpG‐binding domain 10 (MBD10) as phosphoprotein becomes differentially phosphorylated after ABA treatment Arabidopsis. ABA‐induced was delayed mbd10 knockout plants but accelerated MBD10 ‐overexpressing plants, suggesting positively regulates senescence. phosphorylation of occurs planta on Thr‐89, and our results demonstrated Thr‐89 essential for...
Abstract Plants adopt optimal tolerance strategies depending on the intensity and duration of stress. Retaining water is a priority under short-term drought conditions, whereas maintaining growth reproduction processes takes precedence over survival conditions prolonged drought. However, mechanism underlying changes in stress response degree unclear. Here, we report that SNF1-related protein kinase 2 (SnRK2) substrate 1 (SNS1) involved this regulation SNS1 phosphorylated stabilized by SnRK2...
Abstract The Raf-like protein kinase abscisic acid (ABA) and abiotic stress-responsive (ARK) previously identified in the moss Physcomitrium (Physcomitrella) patens acts as an upstream regulator of subgroup III SNF1-related kinase2 (SnRK2), key ABA stress responses. However, mechanisms underlying activation ARK by for regulation SnRK2, including role receptor-associated group A PP2C (PP2C-A), are not understood. We Ser1029 phosphorylation site loop ARK, which provided a possible mechanism...
When plants are exposed to drought stress, there is a trade-off between plant growth and stress responses. Here, we identified signaling mechanism for the initial steps of drought-growth trade-off. Phosphoproteomic profiling revealed that Raf13, B1 subgroup Raf-like kinase, dephosphorylated under conditions. Raf13 related B1-Raf Raf15 required rather than acquisition osmotolerance. We also found interacts with B55-family regulatory subunits protein phosphatase 2A (PP2A), which mediates...
Abstract When plants are exposed to drought stress, there is a trade-off between plant growth and stress responses. Here, we identified signaling mechanism for the initial steps of drought-growth trade-off. Phosphoproteomic profiling revealed that Raf13, B1 subgroup Raf-like kinase, dephosphorylated under conditions. Raf13 related B1-Raf Raf15 required rather than acquisition osmotolerance. We also found interacts with B55-family regulatory subunits protein phosphatase 2A (PP2A), which...
Abstract Abscisic acid (ABA)-induced stomatal closure limits water loss from plants under drought stress. To investigate signaling pathways involved in closure, we performed a phosphoproteomic analysis of ABA-treated Arabidopsis guard cell protoplasts (GCPs). From this analysis, discovered that MITOGEN-ACTIVATED PROTEIN 4 KINASE 1 (MAP4K1) is phosphorylated response to ABA. Subsequent biochemical assays showed Ser-479 MAP4K1 directly by SRK2E (OST1/SnRK2.6), protein kinase plays central role...
ABSTRUCT A phytohormone abscisic acid (ABA) has a major role in abiotic stress responses plants, and subclass III SNF1-related protein kinase 2 (SnRK2) mediates ABA signaling. In this study, we identified Raf36, group C Raf-like Arabidopsis, as an interacting with SnRK2. series of reverse genetic biochemical analyses revealed that Raf36 negatively regulates is directly phosphorylated by SnRK2s. addition, found Raf22, another C-type kinase, functions partially redundantly to regulate...