A5019801309- Plant Stress Responses and Tolerance
- Plant Molecular Biology Research
- Plant nutrient uptake and metabolism
- Bryophyte Studies and Records
- Plant Reproductive Biology
- Botany and Plant Ecology Studies
- Light effects on plants
- Photosynthetic Processes and Mechanisms
- Seed Germination and Physiology
- Biocrusts and Microbial Ecology
- Plant-Microbe Interactions and Immunity
- Plant responses to water stress
- Plant tissue culture and regeneration
- Lichen and fungal ecology
- Marine and coastal plant biology
- Plant Genetic and Mutation Studies
- Wheat and Barley Genetics and Pathology
- Plant Physiology and Cultivation Studies
- Plant Gene Expression Analysis
- Postharvest Quality and Shelf Life Management
- Heat shock proteins research
- Silk-based biomaterials and applications
- Peatlands and Wetlands Ecology
- Neurobiology and Insect Physiology Research
- Research in Cotton Cultivation
Saitama University
2014-2025
Graduate School USA
2018
Tokyo University of Agriculture
2010
Kyoto University
2010
Hokkaido University
1997-2004
Washington State University
1993-1996
University of Washington
1995
Significance Plants can sense loss of water caused by drought and stimulate internal mechanisms for protecting cells from damage with the aid stress hormone abscisic acid (ABA). Analysis a mutant basal land plant, moss Physcomitrella patens , revealed that an impairment protooncogene Raf-like protein kinase, designated “ARK” (for “ABA abiotic stress-responsive kinase”), causes both ABA sensitivity osmotic tolerance. We show evidence ARK has role in integrating signals upstream sucrose...
Vegetative desiccation tolerance is common in bryophytes, although this character has been lost most vascular plants. The moss Physcomitrella patens survives complete if treated with abscisic acid (ABA). Group A protein phosphatases type 2C (PP2C) are negative regulators of signalling. Here we show that the elimination PP2C sufficient to ensure P. survival full desiccation, without ABA treatment, its growth severely hindered. Microarray analysis shows PP2C-regulated genes exclusively overlap...
Abstract The SNF1-related protein kinase 2 (SnRK2) family includes key regulators of osmostress and abscisic acid (ABA) responses in angiosperms can be classified into three subclasses. Subclass III SnRK2s act the ABA response while ABA-nonresponsive subclass I are regulated through osmostress. Here we report that an ancient SnRK2-based signalling module including upstream Raf-like (ARK) exclusively protects moss Physcomitrella patens from drought. both Arabidopsis semiterrestrial alga...
Given their sessile nature, land plants must use various mechanisms to manage dehydration under water-deficit conditions. Osmostress-induced activation of the SNF1-related protein kinase 2 (SnRK2) family elicits physiological responses such as stomatal closure protect during drought With plant hormone ABA receptors [PYR (pyrabactin resistance)/PYL resistance-like)/RCAR (regulatory component receptors) proteins] and group A phosphatases, subclass III SnRK2 also constitutes a core signaling...
Calcium, a universal second messenger, regulates diverse cellular processes in eukaryotes. Ca2+ and Ca2+/calmodulin-regulated protein phosphorylation play pivotal role amplifying diversifying the action of Ca(2+)-binding domain was cloned characterized from lily. The cDNA clone contains an open reading frame coding for 520 amino acids. predicted structure CCaMK catalytic followed by two regulatory domains, calmodulin-binding visinin-like domain. amino-terminal region all 11 conserved...
Abscisic acid (ABA) is postulated to be a ubiquitous hormone that plays central role in seed development and responses environmental stresses of vascular plants. However, liverworts (Marchantiophyta), which represent the oldest extant lineage land plants, ABA has been least emphasized; thus, very little information available on molecular mechanisms underlying responses. In this study, we isolated characterized MpABI1, an ortholog ABSCISIC ACID INSENSITIVE1 (ABI1), from liverwort Marchantia...
A chimeric Ca2+/calmodulin-dependent protein kinase (CCaMK) gene characterized by a catalytic domain, calmodulin-binding and neural visinin-like Ca2+-binding domain was recently cloned from plants (Patil, S., Takezawa, D., Poovaiah, B. W. (1995) Proc. Natl. Acad. Sci. U. S. A. 92, 4797-4801). The Escherichia coli-expressed CCaMK phosphorylates various peptide substrates in manner. region of has similarity to the alpha-subunit multifunctional (CaMKII). exhibits basal autophosphorylation at...
Abscisic acid (ABA) controls seed dormancy and stomatal closure through binding to the intracellular receptor Pyrabactin resistance1 (Pyr1)/Pyr1-like/regulatory components of ABA receptors (PYR/PYL/RCAR) in angiosperms. Genes encoding PYR/PYL/RCAR are thought have arisen ancestor embryophytes, but roles genes nonvascular plants not been determined. In liverwort Marchantia polymorpha, reduces growth enhances desiccation tolerance increasing accumulation sugars various transcripts such as...
Significance SNF1-related protein kinase 2s (SnRK2s) are central regulators in abscisic acid (ABA) signaling and stress responses plants. However, SnRK2 substrates largely unknown. Here, we identified Arabidopsis group C Raf-like kinases Raf36 Raf22 as substrates. Reverse genetic analyses showed that negatively regulate ABA signaling. Under optimal growth conditions, accumulates the cell phosphorylates to suppress responses. Conversely, under abiotic ABA-activated SnRK2s phosphorylate...
Cold acclimation of winter wheat ( Triticum aestivum L.) seedlings induces accumulation in the apoplast taTLPs that are similar to thaumatin‐like proteins (TLPs), which pathogenesis‐related proteins. We characterized a cDNA WAS‐3a encoding major isoform from cells and showed transcripts were markedly increased by treatment with ABA elicitors (chitosan, β ‐glucan cell wall fractions Fusarium oxysporum Microdochium nivale ) cells. To analyse function WAS‐3a, highly efficient expression system...
Cortical parenchyma cells of mulberry (Morus bombycis Koidz.) trees acquire extremely high freezing tolerance in winter as a result seasonal cold acclimation. The amount total proteins endoplasmic reticulum (ER)-enriched fractions isolated from these increased parallel with the process Protein compositions ER-enriched fraction also changed seasonally, prominent accumulation 20-kD (WAP20) and 27-kD (WAP27) winter. N-terminal amino acid sequence WAP20 exhibited homology to ER-localized small...
Summary Plants acclimate to environmental stress signals such as cold, drought and hypersalinity, provoke internal protective mechanisms. Abscisic acid ( ABA ), a carotenoid‐derived phytohormone, which increases in response the above, has been suggested play key role acclimation process angiosperms, but of basal land plants mosses, including its biosynthetic pathways, not clarified. Targeted gene disruption Pp 1 , encoding zeaxanthin epoxidase moss Physcomitrella patens was conducted...
Summary Abscisic acid (ABA) and its signaling system are important for land plants to survive in terrestrial conditions. Here, we took a phosphoproteomic approach elucidate the ABA network Physcomitrella patens , model species of basal plants. Our analysis detected 4630 phosphopeptides from wild‐type P . two ABA‐responsive mutants, disruptant group‐A type‐2C protein phosphatase (PP2C; ppabi1a/b ) AR7, defective mutant ARK, identified as an upstream regulator SnRK2. Quantitative 143 The...
A novel calcium-binding calcium/calmodulin-dependent protein kinase (CCaMK) with a catalytic domain, calmodulin-binding and neural visinin-like domain was cloned characterized from plants [Patil et al., (1995) Proc. Nall. Accad Sci USA 92, 4797–4801; Takezawa al. (1996) J. BioL Chem 271, 8126–8132]. The mechanisms of CCaMK activation by calcium calcium/calmodulin were investigated using various deletion mutants. use mutants lacking either one, two, or all three EF hands indicated that sites...