A5089641766- Plant Stress Responses and Tolerance
- Plant Molecular Biology Research
- Photosynthetic Processes and Mechanisms
- Plant-Microbe Interactions and Immunity
- Plant nutrient uptake and metabolism
- Plant Parasitism and Resistance
- Postharvest Quality and Shelf Life Management
- Plant responses to water stress
- Plant Surface Properties and Treatments
- Mycorrhizal Fungi and Plant Interactions
- RNA Research and Splicing
- Legume Nitrogen Fixing Symbiosis
- Endoplasmic Reticulum Stress and Disease
- Insect and Pesticide Research
- Greenhouse Technology and Climate Control
- RNA regulation and disease
- RNA and protein synthesis mechanisms
- Nitric Oxide and Endothelin Effects
- Heat shock proteins research
- Fungal Biology and Applications
- Mitochondrial Function and Pathology
- RNA modifications and cancer
- Marine and coastal plant biology
- Plant Physiology and Cultivation Studies
- Plant responses to elevated CO2
Tokyo University of Agriculture
2013-2025
RIKEN Center for Sustainable Resource Science
2017-2023
Given their sessile nature, land plants must use various mechanisms to manage dehydration under water-deficit conditions. Osmostress-induced activation of the SNF1-related protein kinase 2 (SnRK2) family elicits physiological responses such as stomatal closure protect during drought With plant hormone ABA receptors [PYR (pyrabactin resistance)/PYL resistance-like)/RCAR (regulatory component receptors) proteins] and group A phosphatases, subclass III SnRK2 also constitutes a core signaling...
Pattern-recognition receptor (PRR)-triggered immunity (PTI) wards off a wide range of pathogenic microbes, playing pivotal role in angiosperms. The model liverwort Marchantia polymorpha triggers defense-related gene expression upon sensing components bacterial and fungal extracts, suggesting the existence PTI this plant model. However, molecular putative M. significance bryophytes have not yet been described. We here show that has four lysin motif (LysM)-domain-containing homologs, two...
Abscisic acid (ABA) controls seed dormancy and stomatal closure through binding to the intracellular receptor Pyrabactin resistance1 (Pyr1)/Pyr1-like/regulatory components of ABA receptors (PYR/PYL/RCAR) in angiosperms. Genes encoding PYR/PYL/RCAR are thought have arisen ancestor embryophytes, but roles genes nonvascular plants not been determined. In liverwort Marchantia polymorpha, reduces growth enhances desiccation tolerance increasing accumulation sugars various transcripts such as...
Abstract The evolution of adaptive interactions with beneficial, neutral and detrimental microbes was one the key features enabling plant terrestrialization. Extensive studies have revealed conserved unique molecular mechanisms underlying plant–microbe across different species; however, most insights gleaned to date been limited seed plants. liverwort Marchantia polymorpha, a descendant early diverging land plants, is gaining in popularity as an advantageous model system understand...
Abstract Bacterial phytopathogens, such as Pseudomonas syringae pv. tomato ( Pst ) DC3000, induce water-soaked lesions in the leaf apoplast under high humidity, facilitating infection. However, it remains largely unclear how plants regulate their resistance to restrict bacterial infection response humidity. Here, we demonstrate that abscisic acid (ABA)-catabolizing ABA 8’-hydroxylase, encoded by CYP707A3 , plays a critical role this Arabidopsis thaliana . Elevated humidity induces...
Summary The phytohormone ABA and the transcription factor ABSCISIC ACID INSENSITIVE 3 ( ABI 3)/ VIVIPAROUS 1 VP 1) function in protecting embryos during desiccation stage of seed development. In a similar signaling pathway, vegetative tissue moss P hyscomitrella patens survives by activating downstream genes (e.g. LEA response to 3. We show that p promoter responds primarily through ACTT ‐core element (5′‐ TCC GTC ‐3′), while ACGT ‐responsive ABRE ) appears respond alone. also found...
Abstract The Raf-like protein kinase abscisic acid (ABA) and abiotic stress-responsive (ARK) previously identified in the moss Physcomitrium (Physcomitrella) patens acts as an upstream regulator of subgroup III SNF1-related kinase2 (SnRK2), key ABA stress responses. However, mechanisms underlying activation ARK by for regulation SnRK2, including role receptor-associated group A PP2C (PP2C-A), are not understood. We Ser1029 phosphorylation site loop ARK, which provided a possible mechanism...
Plants are often exposed not only to short-term (S) heat stress but also long-term (L) over several consecutive days. A few Arabidopsis mutants defective in L-heat tolerance have been identified, the molecular mechanisms involved less well understood than those S-heat tolerance. To elucidate mechanisms, we isolated new sensitive heat5 (sloh5) mutant from EMS-mutagenized seeds of L-heat-tolerant Col-0. The sloh5 was hypersensitive S-heat, osmo-shock, salt-shock or oxidative stress. causal...
Initial exposure of plants to osmotic stress caused by drought, cold, or salinity leads acclimation, termed acquired tolerance, subsequent severe stresses. Acquired osmotolerance induced salt is widespread across Arabidopsis (Arabidopsis thaliana) accessions and conferred disruption a nucleotide-binding leucine-rich repeat gene, designated ACQUIRED OSMOTOLERANCE. De-repression this gene under causes detrimental autoimmunity via ENHANCED DISEASE SUSCEPTIBILITY1 PHYTOALEXIN DEFICIENT4 (PAD4)....
Acquired osmotolerance induced after salt stress is widespread across Arabidopsis thaliana (Arabidopsis) accessions (e.g., Bu-5). However, it remains unclear how this established. Here, we isolated a mutant showing an acquired osmotolerance-defective phenotype (aod2) from ion-beam-mutagenized M2 population of Bu-5. aod2 was impaired not only in but also osmo-shock, salt-shock, and long-term heat tolerances compared with Bu-5, displayed abnormal morphology, including small, wrinkled leaves,...
We have previously reported a wide variation in salt tolerance among Arabidopsis thaliana accessions and identified ACQOS , encoding nucleotide-binding leucine-rich repeat (NLR) protein, as the causal gene responsible for disturbance of acquired osmotolerance induced after mild stress. is conserved osmosensitive accessions, including Col-0. In response to osmotic stress, it induces detrimental autoimmunity, resulting suppression osmotolerance, but how triggers autoimmunity remains unclear....
Abstract Plants are often exposed not only to short-term (S-) heat stress but also diurnal long-term (L-) over several consecutive days. To reveal the mechanisms underlying L-heat tolerance, we here used a forward genetic screen for sensitive (sloh) mutants and isolated sloh4. The mutant was hypersensitive S-heat stress. causal gene of sloh4 identical MIP3 encoding member MAIGO2 (MAG2) tethering complex, which is composed MAG2, MIP1, MIP2 subunits localized at endoplasmic reticulum (ER)...
Acquired osmotolerance induced by initial exposure to mild salt stress is widespread across Arabidopsis thaliana ecotypes, but the mechanism underlying it remains poorly understood. To clarify it, we isolated acquired osmotolerance-deficient 1 (aod1), a mutant highly sensitive osmotic stress, from ion-beam-irradiated seeds of Zu-0, an ecotype known for its remarkably high osmotolerance. Aod1 showed growth inhibition with spotted necrotic lesions on rosette leaves under normal conditions...
Acquired osmotolerance after salt stress is widespread among
Abstract Calcium (Ca2+) is a major ion in living organisms, where it acts as second messenger for various biological phenomena. The Golgi apparatus retains higher Ca2+ concentration than the cytosol and returns cytosolic to basal levels after transient elevation response environmental stimuli such osmotic stress. However, transporters localized of plants have not been clarified. We previously found that wild-type (WT) salt-tolerant Arabidopsis (Arabidopsis thaliana) accession, Bu-5, showed...
Plants are often exposed not only to short-term (S-) but also long-term (L-)heat stress over several consecutive days. A few Arabidopsis mutants defective in L-heat tolerance have been identified, the molecular mechanisms less understood for this than S-heat tolerance. To elucidate of former, we used a forward genetic screen sensitive heat (sloh) and isolated sloh3 sloh63. The were hypersensitive L- stress, sloh63 was salt stress. We identified causal genes, SLOH3 SLOH63, both which encoded...
Natural genetic variation has facilitated the identification of genes underlying complex traits such as stress tolerances. We here evaluated long-term (L-) heat tolerance (37°C for 5 days) 174 Arabidopsis thaliana accessions and short-term (S-) (42°C, 50 min) 88 found extensive variation, respectively. Interestingly, L-heat-tolerant are not necessarily S-heat tolerant, suggesting that mechanisms different. To elucidate we performed a chromosomal mapping using F2 progeny cross between Ms-0 (a...
ABSTRACT The Raf-like protein kinase ARK previously identified in the moss Physcomitrella patens acts as an upstream regulator of subgroup III SnRK2, key abscisic acid (ABA) and abiotic stress responses. However, mechanisms underlying activation by ABA for regulation SnRK2 including role receptor-associated group A PP2C (PP2C-A) are not understood. We Ser1029 phosphorylation site loop ARK, which provided a possible mechanism its activity. Analysis transgenic ark lines expressing ARK-GFP with...
Abstract Pattern-recognition receptor (PRR)-triggered immunity (PTI) wards off a wide range of pathogenic microbes, playing pivotal role in plant immunity. The model liverwort Marchantia polymorpha is emerging as popular for investigating the evolution plant-microbe interactions. M. triggers defense-related gene expression upon sensing components bacterial and fungal extracts, suggesting existence PTI this model. However, molecular putative have not yet been described. We show that, , which...