A5016227945- Plant Molecular Biology Research
- Plant nutrient uptake and metabolism
- Plant Reproductive Biology
- Plant Stress Responses and Tolerance
- Plant tissue culture and regeneration
- Polysaccharides and Plant Cell Walls
- Plant-Microbe Interactions and Immunity
- Plant Gene Expression Analysis
- Photosynthetic Processes and Mechanisms
- Legume Nitrogen Fixing Symbiosis
- Plant responses to water stress
- Polysaccharides Composition and Applications
- 14-3-3 protein interactions
Tokyo University of Agriculture and Technology
2019-2024
Institut Jean-Pierre Bourgin
2023
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2023
Biopolymères Interactions Assemblages
2023
Abstract Inactivation of the phytohormone auxin plays important roles in plant development, and several enzymes have been implicated inactivation. In this study, we show that predominant natural auxin, indole-3-acetic acid (IAA), is mainly inactivated via GH3-ILR1-DAO pathway. IAA first converted to IAA-amino conjugates by GH3 IAA-amidosynthetases. The IAA-aspartate (IAA-Asp) IAA-glutamate (IAA-Glu) are storage forms can be back ILR1/ILL amidohydrolases. We further DAO1 dioxygenase...
Auxin is the first discovered plant hormone and essential for many aspects of growth development. Indole-3-acetic acid (IAA) main auxin plays pivotal roles in intercellular communication through polar transport. Phenylacetic (PAA) another natural that does not show movement. Although a wide range species have been shown to produce PAA, its biosynthesis, inactivation physiological significance plants are largely unknown. In this study, we demonstrate overexpression CYP79A2 gene, which...
The phytohormone auxin, indole-3-acetic acid (IAA), plays a prominent role in plant development. Auxin homeostasis is coordinately regulated by auxin synthesis, transport, and inactivation; however, the physiological contribution of inactivation to has not been determined. GH3 IAA–amino conjugating enzymes play central inactivation. Chemical inhibition proteins planta challenging because these leads IAA overaccumulation that rapidly induces expression. Here, we report characterization potent...
Significance Plant roots grow toward gravity. The phenomenon is known as “root gravitropism” early described by Charles Darwin, and it has been well established that the plant hormone auxin plays a central role in this response. major endogenous auxin, indole-3-acetic acid (IAA), mainly synthesized from tryptophan via indole-3-pyruvic acid, whereas indole-3-butyric (IBA) minor precursor of IAA. Previous studies have shown IAA derived IBA specific regulating lateral root development. However,...
Gretchen Hagen 3 (GH3) amido synthetases conjugate amino acids to a carboxyl group of small molecules including hormones auxin, jasmonate, and salicylic acid. The Arabidopsis genome harbors 19 GH3 genes, whose exact roles in plant development have been difficult define because genetic redundancy among the genes. Here we use CRISPR/Cas9 gene editing technology delete II which are able indole-3-acetic acid (IAA) acids. We show that plants lacking eight genes (gh3 octuple mutants) accumulate...
Abstract Plants adopt optimal tolerance strategies depending on the intensity and duration of stress. Retaining water is a priority under short-term drought conditions, whereas maintaining growth reproduction processes takes precedence over survival conditions prolonged drought. However, mechanism underlying changes in stress response degree unclear. Here, we report that SNF1-related protein kinase 2 (SnRK2) substrate 1 (SNS1) involved this regulation SNS1 phosphorylated stabilized by SnRK2...
Abstract Auxin is a key regulator of plant growth and development. Indole-3-acetic acid (IAA), auxin, mainly produced from tryptophan via indole-3-pyruvate (IPA) in both bryophytes angiosperms. Angiosperms have multiple, well-documented IAA inactivation pathways, involving conjugation to IAA-aspartate (IAA-Asp)/glutamate by the GH3 auxin-amido synthetases, oxidation 2-oxindole-3-acetic (oxIAA) DAO proteins. However, biosynthesis processes remain elusive lycophytes, an early lineage...
4-Phenylbutyric acid (4PBA) is utilized as a drug to treat urea cycle disorders and also being studied potential anticancer that acts via its histone deacetylase (HDAC) inhibitor activity. During search find small molecules affect plant regeneration in Arabidopsis, we found 4PBA treatment promotes this process by mimicking the effect of exogenous auxin. Specifically, tissue culture experiments revealed medium containing enhances callus formation subsequent shoot regeneration. Analyses with...
Abstract Several plant-associated microbes synthesize the auxinic plant growth regulator phenylacetic acid (PAA) in culture; however, role of PAA plant-pathogen interactions is not well understood. In this study, we investigate during between phytopathogenic bacterium Pseudomonas syringae strain Pto DC3000 ( DC3000) and model host, Arabidopsis thaliana . Previous work demonstrated that indole-3-acetaldehyde dehydrogenase A (AldA) converts (IAAld) to auxin indole-3-acetic (IAA). Here, further...
Several plant-associated microbes synthesize the auxinic plant growth regulator phenylacetic acid (PAA) in culture; however, role of PAA plant-pathogen interactions is not well understood. In this study, we investigated during between phytopathogenic bacterium Pseudomonas syringae strain Pto DC3000 ( DC3000) and model host, Arabidopsis thaliana . Previous work demonstrated that indole-3-acetaldehyde dehydrogenase A (AldA) converts (IAAld) to auxin indole-3-acetic (IAA). Here, further...
Abstract During Arabidopsis seed coat development, copious amounts of mucilage polysaccharides are produced in the epidermal cells. When hydrated on imbibition, these expand and released to encapsulate as a two-layered hydrogel. Polysaccharides synthesized from UDP-sugars by glycosyltransferases (GTs) several GTs, with differing activities, have been identified that contribute polysaccharide synthesis. How GTs orchestrate production complex found remains be determined. In this study, we...
Abstract Active membrane transport of plant hormones and their related compounds is an essential process that determines the distribution within tissues and, hence, regulates various physiological events. Here, we report Arabidopsis NITRATE TRANSPORTER 1/PEPTIDE FAMILY 7.3 (NPF7.3) protein functions as a transporter indole-3-butyric acid (IBA), precursor major endogenous auxin indole-3-acetic (IAA). When expressed in yeast, NPF7.3 mediated cellular IBA uptake. Loss-of-function npf7.3 mutants...
Abstract The phytohormone auxin, specifically indole-3-acetic acid (IAA) plays a prominent role in plant development. Cellular auxin concentration is coordinately regulated by synthesis, transport, and inactivation to maintain homeostasis; however, the physiological contribution of homeostasis has remained elusive. GH3 genes encode amino conjugating enzymes that perform central inactivation. chemical inhibition GH3s planta challenging because leads IAA overaccumulation rapidly induces...
Abstract Gretchen Hagen 3 (GH3) amido synthetases conjugate amino acids to a carboxyl group of small molecules including hormones auxin, jasmonate, and salicylic acid. The Arabidopsis genome harbors 19 GH3 genes, whose exact roles in plant development have been difficult define because genetic redundancy among the genes. Here we use CRISPR/Cas9 gene editing technology delete II which are able indole-3-acetic acid (IAA) acids. We show that plants lacking eight genes ( gh3 octuple mutants)...