A5050748026- Plant Molecular Biology Research
- Neurogenesis and neuroplasticity mechanisms
- Plant nutrient uptake and metabolism
- Neuroscience and Neuropharmacology Research
- Plant Stress Responses and Tolerance
- Plant Reproductive Biology
- Photosynthetic Processes and Mechanisms
- Light effects on plants
- Analytical Chemistry and Chromatography
- RNA Research and Splicing
- Axon Guidance and Neuronal Signaling
- Memory and Neural Mechanisms
- MicroRNA in disease regulation
- Humic Substances and Bio-Organic Studies
- Plant-Derived Bioactive Compounds
- Biochemical and Structural Characterization
- Plant Gene Expression Analysis
- Caveolin-1 and cellular processes
- Metabolomics and Mass Spectrometry Studies
- Photoreceptor and optogenetics research
- Probiotics and Fermented Foods
- Polysaccharides and Plant Cell Walls
- Microtubule and mitosis dynamics
- Soybean genetics and cultivation
- Parasitic Infections and Diagnostics
Université Paris-Saclay
2019-2025
RIKEN Center for Sustainable Resource Science
2020-2025
Institut Jean-Pierre Bourgin
2019-2025
AgroParisTech
2019-2025
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2025
Tokyo Metropolitan University
2013-2019
Centre National de la Recherche Scientifique
2019
Japan Society for the Promotion of Science
2019
Kyoto University
1991-2018
RIKEN Center for Brain Science
2005-2012
Collapsin response mediating protein‐2 (CRMP2) has been identified as an intracellular protein Semaphorin3A (Sema3A), a repulsive guidance molecule. In this study, we demonstrate that cyclin‐dependent kinase 5 (Cdk5) and glycogen synthase 3β (GSK3β) plays critical role in Sema3A signalling. vitro assay, Cdk5 phosphorylated CRMP2 at Ser522, while GSK3β did not induce any phosphorylation of CRMP2. Phosphorylation by was exclusively observed Cdk5‐phosphorylated CRMP2, but barely CRMP2T509A....
Summary Indole‐3–acetic acid ( IAA ), an auxin plant hormone, is biosynthesized from tryptophan. The indole‐3–pyruvic IP y A ) pathway, involving the tryptophan aminotransferase TAA 1 and YUCCA YUC enzymes, was recently found to be a major biosynthetic pathway in Arabidopsis. catalyzes conversion of , produces . Using chemical biology approach with maize coleoptiles, we identified 5–(4–chlorophenyl)‐4H‐1,2,4–triazole‐3–thiol (yucasin) as potent inhibitor biosynthesis ‐expressing coleoptile...
The mammalian cerebral cortex consists of six layers that are generated via coordinated neuronal migration during the embryonic period. Recent studies identified specific phases radial cortical neurons. After final division, neurons transform from a multipolar to bipolar shape within subventricular zone-intermediate zone (SVZ-IZ) and then migrate along glial fibres. Mice lacking Cdk5 exhibit abnormal corticogenesis owing defects. When we introduced GFP into migrating at E14.5 by in utero...
Abscisic acid (ABA) is an important hormone for seed development and germination whose physiological action modulated by its endogenous levels. Cleavage of carotenoid precursors 9-cis epoxycarotenoid dioxygenase (NCED) inactivation ABA 8′-hydroxylase (CYP707A) are key regulatory metabolic steps. In Arabidopsis (Arabidopsis thaliana), both enzymes encoded multigene families, having distinctive expression patterns. To evaluate the genome-wide impact deficiency in developing seeds at maturation...
Significance Plant roots grow toward gravity. The phenomenon is known as “root gravitropism” early described by Charles Darwin, and it has been well established that the plant hormone auxin plays a central role in this response. major endogenous auxin, indole-3-acetic acid (IAA), mainly synthesized from tryptophan via indole-3-pyruvic acid, whereas indole-3-butyric (IBA) minor precursor of IAA. Previous studies have shown IAA derived IBA specific regulating lateral root development. However,...
SUMMARY Seed dormancy is an adaptive trait that delays germination until environmental conditions become favorable for seedling survival and growth. Germination has been shown to depend on the mechanical resistance strength of covering layers (testa endosperm) counteracts growth force embryo. Cell wall remodeling essential in regulation processes. In Arabidopsis thaliana , side chain trimming xyloglucans (XyG), major hemicellulose cell walls, by apoplastic XYLOSIDASE1 (XYL1), previously...
Abstract Cdk5 (cyclin‐dependent kinase 5) activity is dependent upon association with one of two neuron‐specific activators, p35 or p39. Genetic deletion causes perinatal lethality severe defects in corticogenesis and neuronal positioning. –/– mice are viable milder histological abnormalities. Although substantial evidence implicates synaptic plasticity, its role learning memory has not been evaluated using mutant mouse models. We report here that have deficiencies spatial memory. Close...
The plant hormone abscisic acid (ABA) is actively synthesized in vascular tissues and transported to guard cells promote stomatal closure. Although several transmembrane ABA transporters have been identified, how the movement of within plants regulated not fully understood. In this study, we determined that Arabidopsis NPF4.6, previously identified as an transporter expressed tissues, also present positively regulates closure leaves. We found mutants defective NPF5.1 had a higher leaf...
Gravitropism refers to the growth or movement of plants that is influenced by gravity. Roots exhibit positive gravitropism, and root cap thought be gravity-sensing site. In some plants, requires light irradiation for gravitropic responses. However, mechanisms regulating this phenomenon are unknown. We herein report maize roots exposed white continuously ≥1–2h show increased indole-3-acetic acid (IAA) levels in tips, especially transition zone (1–3mm from tip). Treatment with IAA biosynthesis...
Abstract Doublecortin (DCX) is expressed in young neurons and functions as a microtubule‐associated protein. DCX essential for neuronal migration because humans with mutations the gene exhibit cortical lamination defects known lissencephaly males subcortical laminar heterotopia (or double cortex syndrome) females. Phosphorylation of alters its affinity tubulin may modulate neurite extension migra tion. Previous vitro phosphorylation experiments revealed that cyclin‐dependent kinase 5 (Cdk5)...
Learning and memory processes are similarly organized in humans monkeys; therefore, monkeys can be ideal models for analyzing human aging neurodegenerative diseases such as Alzheimer's disease. With the development of novel gene modification methods, common marmosets (Callithrix jacchus) have been suggested an animal model diseases. Furthermore, marmoset's lifespan is relatively short, which makes it a practical aging. Working deficits prominent symptom both dementia aging, but no data...
Phototropin1 (phot1) perceives low- to high-fluence blue light stimuli and mediates both the first second positive phototropisms. High-fluence is known induce autophosphorylation of phot1, leading phototropism. However, phosphorylation status phot1 by low-fluence that induces phototropism had not been observed. Here, we conducted a phosphoproteomic analysis maize coleoptiles investigate fluence-dependent Zmphot1. induced Zmphot1 at several sites. Notably, significantly increased level Ser291...
Plant roots play important roles in absorbing water and nutrients, tolerance against environmental stresses. Previously, we identified a rice root-specific pathogenesis-related protein (RSOsPR10) induced by drought, salt, wounding. RSOsPR10 expression is strongly jasmonate (JA)/ethylene (ET), but suppressed salicylic acid (SA). Here, analyzed the promoter activity of RSOsPR10. Analyses transgenic lines harboring different-length promoter::β-glucuronidase (GUS) constructs showed that 3-kb...
A representative mucilage, named Althaea-mucilage OL, has been isolated from the leaves of Althaea officinalis L. The final Preparation was homogeneous as determined by ultracentrifugal analysis, cellulose acetate membrane electrophoresis, and gel chromatography. Its water solution gave an intrinsic viscosity value 49.0. It mainly composed partially acetylated acidic polysaccharide, its molecular weight estimated to be about 1800000. polysaccharide L-rhamnose : D-galacturonic acid...
A division arrest mutant, cdaA, of Tetrahymena thermophila is known to have a temperature sensitive-defect in the determination plane, and its gene product had been shown be protein designated as p85 (Mr=85,000; pI=4.7). Here localization was accessary structure specialized basal body close furrow by immunoelectron microscopy using anti-p85 antiserum.
Studies using inhibitors of indole-3-acetic acid (IAA) transport, not only for efflux but influx carriers, provide many aspects auxin physiology in plants. 1-Naphtoxyacetic (1-NOA), an analog the synthetic 1-N-naphtalene acetic (NAA), inhibits IAA carrier AUX1. However, 1-NOA also shows activity because its structural similarity to NAA. In this study, we have identified another candidate inhibitor carrier. The compound, “7-B3; ethyl 2-[(2-chloro-4-nitrophenyl)thio]acetate,” is a...
Abstract Active membrane transport of plant hormones and their related compounds is an essential process that determines the distribution within tissues and, hence, regulates various physiological events. Here, we report Arabidopsis NITRATE TRANSPORTER 1/PEPTIDE FAMILY 7.3 (NPF7.3) protein functions as a transporter indole-3-butyric acid (IBA), precursor major endogenous auxin indole-3-acetic (IAA). When expressed in yeast, NPF7.3 mediated cellular IBA uptake. Loss-of-function npf7.3 mutants...
千葉県小櫃川・小糸川流域の民家において, ミツバツツジ類植栽木の生育状況と自然発生実生の調査を行った。ミツバツツジ類はヤマツツジより樹勢が悪かった。数量化理論1類の解析結果より, キヨスミミツバツツジは排水良好で低温多湿の壌土のところ, ミツバツツジは排水良好の埴壌土で西日を遮るものがあるところ, ヤマツツジは根系が制限されない立地で, 低温多湿の酸性土壌が生育に適した環境であるということがわかった。また, 実生はターフ型のコケ上に多く分布しており, ミツバツツジ類は半日陰の壌土か埴壌土のところ, ヤマツツジは日陰で壌土のところで発芽・更新するということがわかった。
Cystine formation from various S-protected cysteine derivatives by treatment with combinations of sulphoxides and silyl compounds in trifluoroacetic acid was examined, selectivity disulphide bond forming found these systems.