A5006341320- Seed Germination and Physiology
- Soybean genetics and cultivation
- Plant Stress Responses and Tolerance
- Plant Genetic and Mutation Studies
- Photosynthetic Processes and Mechanisms
- Plant tissue culture and regeneration
- Plant Molecular Biology Research
- Plant nutrient uptake and metabolism
- Genetic Mapping and Diversity in Plants and Animals
- Plant Parasitism and Resistance
- Plant Reproductive Biology
- African Botany and Ecology Studies
- Genetics and Plant Breeding
- Plant biochemistry and biosynthesis
- Lipid metabolism and biosynthesis
- Legume Nitrogen Fixing Symbiosis
- Magnetic and Electromagnetic Effects
- Plant Gene Expression Analysis
- GABA and Rice Research
- Growth and nutrition in plants
- Allelopathy and phytotoxic interactions
- Agricultural pest management studies
- Food composition and properties
- Agriculture and Rural Development Research
- Plant responses to water stress
Institut Jean-Pierre Bourgin
2016-2025
AgroParisTech
2015-2025
Université Paris-Saclay
2016-2025
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2019-2025
Centre National de la Recherche Scientifique
2005-2019
Bayer (France)
2004-2017
Université Claude Bernard Lyon 1
2008-2016
Institut National de la Recherche Agronomique
2009-2013
ParisTech
2012
Bayer (Germany)
2007
Abstract A variety of mechanisms have been proposed to account for the extension life span in seeds (seed longevity). In this work, we used Arabidopsis (Arabidopsis thaliana) as a model and carried out differential proteomics investigate trait, which is both ecological agricultural importance. our system based on controlled deterioration treatment (CDT), compared seed samples treated different periods time up 7 d. Germination tests showed progressive decrease germination vigor depending...
Abstract To investigate the role of stored and neosynthesized mRNAs in seed germination, we examined effect α-amanitin, a transcriptional inhibitor targeting RNA polymerase II, on germination nondormant Arabidopsis seeds. We used transparent testa mutants, which coat is highly permeable, to better ascertain that drug can reach embryo during imbibition. Even with most permeable mutant (tt2-1), (radicle protrusion) occurred absence transcription, while subsequent seedling growth was blocked....
Abstract The influence of salicylic acid (SA) on elicitation defense mechanisms in Arabidopsis (Arabidopsis thaliana) seeds and seedlings was assessed by physiological measurements combined with global expression profiling (proteomics). Parallel experiments were carried out using the NahG transgenic plants expressing bacterial gene encoding SA hydroxylase, which cannot accumulate active form this plant elicitor. markedly improved germination under salt stress. Proteomic analyses disclosed a...
Abstract Increased cellular levels of reactive oxygen species are known to occur during seed development and germination, but the consequences in terms protein degradation poorly characterized. In this work, carbonylation, which is an irreversible oxidation process leading a loss function modified proteins, has been analyzed by proteomic approach first stages Arabidopsis (Arabidopsis thaliana) germination. dry mature seeds, legumin-type globulins (12S cruciferins) were major targets....
Hydrogen peroxide (H2O2) and nitric oxide (˙NO) are key reactive species in signal transduction pathways leading to activation of plant defense against biotic or abiotic stress. Here, we investigated the effect pre-treating citrus plants (Citrus aurantium L.) with either these two molecules on acclimation salinity show that both pre-treatments strongly reduced detrimental phenotypical physiological effects accompanying this A proteomic analysis disclosed 85 leaf proteins underwent...
Dormancy is an adaptive trait that enables seed germination to coincide with favorable environmental conditions. It has been clearly demonstrated dormancy induced by abscisic acid (ABA) during development on the mother plant. After dispersal, preceded a decline in ABA imbibed seeds, which results from catabolism through 8'-hydroxylation. The hormonal balance between and gibberellins (GAs) shown act as integrator of cues maintain or activate germination. interplay other endogenous signals...
Summary The seed expressed gene DELAY OF GERMINATION ( DOG ) 1 is absolutely required for the induction of dormancy. Next to a non‐dormant phenotype, dog1‐1 mutant also characterized by reduced longevity suggesting that may affect additional processes as well. This aspect however, has been hardly studied and poorly understood. To uncover roles in seeds we performed detailed analysis dog1 using both transcriptomics metabolomics investigate molecular consequences dysfunctional gene. Further,...
During seed germination, the transition from a quiescent metabolic state in dry mature to proliferative vigorous seedling is crucial for plant propagation as well optimizing crop yield. This work provides detailed description of dynamics protein synthesis during time course demonstrating that mRNA translation both sequential and selective this process. The complete inhibition germination process presence inhibitor cycloheximide established critical Arabidopsis germination. However, turnover...
Proteomics approaches have been a useful tool for determining the biological roles and functions of individual proteins identifying molecular mechanisms that govern seed germination, vigour viability in response to ageing. In this work dry proteome four Arabidopsis thaliana genotypes, carry introgression fragments at position longevity quantitative trait loci as result display different levels longevity, was investigated. Seeds two physiological states, after-ripened seeds had full...
Germination is a traditional process and re-emerging trend in healthy foods, resulting progressively increase scientific research on their nutritional traits phytochemical contents. This review examines the physiological biochemical changes during germination sensu stricto pulses, taking into consideration genotype, environmental conditions, hormone control, metabolic transition from seed to seedling. achieved as soon elongation of radicle, implies heterotrophy metabolism; further thermal...
SUMMARY Seeds of Brassicaceae produce a large diversity beneficial and antinutritional specialized metabolites (SMs) that influence their quality provide resistance to stresses. While SM distribution has been described in leaves root tissues, limited information is available about spatiotemporal accumulation seeds. Camelina sativa (camelina) an oilseed cultivated for human animal nutrition industrial uses. we previously explored plasticity, no expression related proteins genes camelina In...
Abscisic acid (ABA) is an important hormone for seed development and germination whose physiological action modulated by its endogenous levels. Cleavage of carotenoid precursors 9-cis epoxycarotenoid dioxygenase (NCED) inactivation ABA 8′-hydroxylase (CYP707A) are key regulatory metabolic steps. In Arabidopsis (Arabidopsis thaliana), both enzymes encoded multigene families, having distinctive expression patterns. To evaluate the genome-wide impact deficiency in developing seeds at maturation...
Autophagy is essential for protein degradation, nutrient recycling, and nitrogen remobilization. induced during leaf ageing in response to starvation, known play a fundamental role recycling remobilization seed filling. Accordingly, leaves of Arabidopsis autophagy mutants (atg) have been shown over-accumulate proteins peptides, possibly because reduced degradation capacity. Surprisingly, atg also displayed higher protease activities. The work reported here aimed at identifying the nature...
Summary Autophagy is a universal mechanism in eukaryotic cells that facilitates the degradation of unwanted cell constituents and essential for homeostasis nutrient recycling. The salicylic acid‐independent effects autophagy defects on leaf metabolism were determined through large‐scale proteomic lipidomic analyses atg5 atg5/sid2 mutants under different nitrogen sulfur growth conditions. Results revealed irrespective conditions, plants carrying mutation presented all characteristics...
Diversity, functions and related enzymes of Specialized Metabolite (SM) modifications from plants seeds Brassicaceae are summarized in this review. A particular focus is made on SM plasticity modification seeds.
• Seed longevity is an important trait in many crops and essential for the success of most land plant species. Current knowledge its molecular regulation limited. The Arabidopsis mutants abscisic acid insensitive3-5 (abi3-5) leafy cotyledon1-3 (lec1-3) have impaired seed maturation quickly lose viability. abi3-5 lec1-3 were used as sensitized genetic backgrounds study longevity. We exploited natural variation to create introgression lines from Seis am Schlern Shahdara accessions in,...