A5060946965- Plant nutrient uptake and metabolism
- Autophagy in Disease and Therapy
- Plant Molecular Biology Research
- Photosynthetic Processes and Mechanisms
- Nitrogen and Sulfur Effects on Brassica
- Legume Nitrogen Fixing Symbiosis
- Plant Stress Responses and Tolerance
- Plant responses to water stress
- Leaf Properties and Growth Measurement
- Phytase and its Applications
- Crop Yield and Soil Fertility
- Plant Physiology and Cultivation Studies
- Plant Micronutrient Interactions and Effects
- Soil Carbon and Nitrogen Dynamics
- Growth and nutrition in plants
- Rice Cultivation and Yield Improvement
- Studies on Chitinases and Chitosanases
- Genetic Mapping and Diversity in Plants and Animals
- Plant Disease Resistance and Genetics
- Wheat and Barley Genetics and Pathology
- Plant-Microbe Interactions and Immunity
- Caching and Content Delivery
- Metalloenzymes and iron-sulfur proteins
- Agriculture, Plant Science, Crop Management
- Greenhouse Technology and Climate Control
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2017-2024
AgroParisTech
2012-2024
Institut Jean-Pierre Bourgin
2013-2024
Université Paris-Saclay
2016-2024
Centre National de la Recherche Scientifique
2018
Institut National de la Recherche Agronomique
2012-2014
• Processes allowing the recycling of organic nitrogen and export to young leaves seeds are important determinants plant yield, especially when plants nitrate-limited. Because autophagy is induced during leaf ageing in response starvation, its role remobilization was suspected. It recently shown that participates trafficking Rubisco-containing bodies vacuole. To investigate remobilization, several autophagy-defective (atg) Arabidopsis mutants were grown under low high nitrate supplies...
Abstract Autophagy is a fundamental process in the plant life story, playing key role immunity, senescence, nutrient recycling, and adaptation to environment. Transcriptomics metabolomics of rosette leaves Arabidopsis thaliana autophagy mutants (atg) show that essential for cell homeostasis stress responses several metabolic pathways are affected. Depletion hexoses, quercetins, anthocyanins parallel overaccumulation amino acids related compounds, such as glutamate, methionine, glutathione,...
Summary Autophagy is present at a basal level in all plant tissues and induced during leaf ageing response to nitrogen ( N ) starvation. Nitrogen remobilization from the rosette seeds impaired autophagy mutants. This report focuses on role of management proteolysis ageing. Metabolites, enzyme activities protein contents were monitored several autophagy‐defective atg Arabidopsis mutants grown under low high nitrate conditions. Results showed that carbon C statuses affected before any...
We investigated the function of ASN2, one three genes encoding asparagine synthetase (EC 6.3.5.4), which is most highly expressed in vegetative leaves Arabidopsis thaliana. Expression ASN2 and parallel higher content darkness suggest that leaf metabolism involves for synthesis. In asn2-1 knockout asn2-2 knockdown lines, disruption caused a defective growth phenotype ammonium accumulation. The asn2 mutant displayed depleted an accumulation alanine, GABA, pyruvate fumarate, indicating alanine...
Oilseed rape is the third largest oleaginous crop in world but requires high levels of N fertilizer which only 50% recovered seeds. This weak use efficiency associated with a low foliar remobilization, leading to significant return soil and risk pollution. Contrary what observed during senescence vegetative stages, remobilization from stems leaves considered efficient monocarpic senescence. However, contribution towards management cellular mechanisms involved remain largely unknown. To reach...
Autophagy knock-out mutants in maize and Arabidopsis are impaired nitrogen (N) recycling exhibit reduced levels of N remobilization to their seeds. It is thus impoortant determine whether higher autophagy activity could, conversely, improve efficiency seed protein content, under what circumstances. As the machinery involves many genes amongst which 18 important for core machinery, choice AUTOPHAGY (ATG) gene manipulate increase was examined. We choose ATG8 overexpression since it has been...
Autophagy is essential for protein degradation, nutrient recycling, and nitrogen remobilization. induced during leaf ageing in response to starvation, known play a fundamental role recycling remobilization seed filling. Accordingly, leaves of Arabidopsis autophagy mutants (atg) have been shown over-accumulate proteins peptides, possibly because reduced degradation capacity. Surprisingly, atg also displayed higher protease activities. The work reported here aimed at identifying the nature...
Glutamine synthetase (GS) is central for ammonium assimilation and consists of cytosolic (GS1) chloroplastic (GS2) isoenzymes. During plant ageing, GS2 protein decreases due to chloroplast degradation, GS1 activity increases support glutamine biosynthesis N remobilization from senescing leaves. The role the different Arabidopsis isoforms in nitrogen was examined using 15N tracing experiments. Only gln1;1-gln1;2-gln1;3 triple-mutation affecting three GLN1;1, GLN1;2, GLN1;3 genes significantly...
Despite a general view that asparagine synthetase generates as an amino acid for long-distance transport of nitrogen to sink organs, its role in metabolic pathways floral organs during seed filling has remained undefined. We demonstrate the onset pollination Arabidopsis induces selected genes metabolism, namely ASN1 (At3g47340), GLN2 (At5g35630), GLU1 (At5g04140), AapAT2 (At5g19950), ASPGA1 (At5g08100) and ASPGB1 (At3g16150), particularly at ovule stage (stage 0), accompanied by enhanced...
Leaf senescence is a long developmental process important for nutrient management and source to sink remobilization. Constituents of the mesophyll cells are progressively degraded provide nutrients rest plant. Up now, studies on leaf have not paid much attention role different tissues. In present study, we dissected laminae from midvein perform metabolite profiling. The nutrients, in C3 plants they contain Rubisco as most nitrogen storage pool. Veins, rich vasculature, place where all...
Owing to the large genetic diversity of barley and its resilience under harsh environments, this crop is great value for agroecological transition need reduction nitrogen (N) fertilizers inputs. In present work, we investigated a North African genotype collection in terms growth limiting N (LN) or ample (HN) supply physiological traits including amino acid content young seedlings. We identified Moroccan variety, Laanaceur, accumulating five times more lysine leaves than others both...
Barley is a cereal of primary importance for forage and human nutrition, useful model wheat. Autophagy genes first described in yeast have been subsequently isolated mammals Arabidopsis thaliana. In maize it was recently shown that autophagy machinery participates nitrogen remobilization grain filling. rice, also important recycling at the vegetative stage. this study, HvATGs, HvNBR1 HvATI1 sequences were identified from bacterial artificial chromosome (BAC), complementary DNA (cDNA)...
Sulphur deficiency in crops became an agricultural concern several decades ago, due to the decrease of S deposition and atmospheric sulphur dioxide emissions released by industrial plants. Autophagy, which is a conserved mechanism for nutrient recycling eukaryotes, involved nitrogen, iron, zinc manganese remobilizations from rosette seeds Arabidopsis thaliana. Here, we have compared role autophagy nitrogen management at whole plant level, performing concurrent labelling with 34S 15N isotopes...
SUMMARY The SID2 (SA INDUCTION‐DEFICIENT2) gene that encodes ICS1 (isochorismate synthase), plays a central role in salicylic acid biosynthesis Arabidopsis. sid2 and NahG (encoding bacterial SA hydroxylase) overexpressing mutants (NahG‐OE) have currently been shown to outperform wild type, presenting delayed leaf senescence, higher plant biomass better seed yield. When grown under sulfate‐limited conditions (low‐S), exhibited early yellowing compared the NahG‐OE, npr1 mutant affected...