A5061088407- Plant Molecular Biology Research
- Plant Reproductive Biology
- Plant nutrient uptake and metabolism
- Plant tissue culture and regeneration
- Natural product bioactivities and synthesis
- Photosynthetic Processes and Mechanisms
- Light effects on plants
- Microbial Natural Products and Biosynthesis
- Plant Parasitism and Resistance
- Sesquiterpenes and Asteraceae Studies
- Plant biochemistry and biosynthesis
- Biological Stains and Phytochemicals
- Protein Degradation and Inhibitors
- Plant Stress Responses and Tolerance
- Ubiquitin and proteasome pathways
- Plant Gene Expression Analysis
- Mitochondrial Function and Pathology
- Bioactive Compounds and Antitumor Agents
- Plant-Derived Bioactive Compounds
- Fungal Biology and Applications
- Plant-Microbe Interactions and Immunity
- Polysaccharides and Plant Cell Walls
- X-ray Diffraction in Crystallography
- Synthesis and Biological Activity
- Marine Sponges and Natural Products
Okayama University of Science
2015-2024
Nagoya University
2012
Tokyo University of Agriculture
2010
National Institute for Basic Biology
2010
Saga University
2009
La Jolla Institute for Immunology
2007
Japan Atomic Energy Agency
2006
Rockefeller University
2005
Kaohsiung Medical University
2003-2005
Okayama University
2005
The phytohormone auxin plays critical roles in the regulation of plant growth and development. Indole-3-acetic acid (IAA) has been recognized as major for more than 70 y. Although several pathways have proposed, how is synthesized plants still unclear. Previous genetic enzymatic studies demonstrated that both TRYPTOPHAN AMINOTRANSFERASE OF ARABIDOPSIS (TAA) YUCCA (YUC) flavin monooxygenase-like proteins are required biosynthesis IAA during development, but these enzymes were placed two...
Abstract Protein knockdown using the auxin-inducible degron (AID) technology is useful to study protein function in living cells because it induces rapid depletion, which makes possible observe an immediate phenotype. However, current AID system has two major drawbacks: leaky degradation and requirement for a high dose of auxin. These negative features make difficult control precisely expression level interest apply this method mice. Here, we overcome these problems by taking advantage...
Abstract The phytohormone auxin is a major regulator of diverse aspects plant growth and development. ubiquitin-ligase complex SCFTIR1/AFB (for Skp1-Cul1-F-box protein), which includes the TRANSPORT INHIBITOR RESPONSE1/AUXIN SIGNALING F-BOX (TIR1/AFB) receptor family, has recently been demonstrated to be critical for auxin-mediated transcriptional regulation. Early-phase auxin-induced hypocotyl elongation, on other hand, long explained by acid-growth theory, proton extrusion plasma membrane...
Abstract Inactivation of the phytohormone auxin plays important roles in plant development, and several enzymes have been implicated inactivation. In this study, we show that predominant natural auxin, indole-3-acetic acid (IAA), is mainly inactivated via GH3-ILR1-DAO pathway. IAA first converted to IAA-amino conjugates by GH3 IAA-amidosynthetases. The IAA-aspartate (IAA-Asp) IAA-glutamate (IAA-Glu) are storage forms can be back ILR1/ILL amidohydrolases. We further DAO1 dioxygenase...
The plant hormone auxin is a master regulator of growth and development. By regulating rates cell division elongation triggering specific patterning events, indole 3-acetic acid (IAA) regulates almost every aspect perception involves the formation ternary complex consisting an F-box protein TIR1/AFB family receptors, molecule, member Aux/IAA co-repressor proteins. In this study, we identified potent antagonist, α-(phenylethyl-2-oxo)-IAA, as lead compound for receptors by in silico virtual...
The regulation of gene expression by the hormone auxin is a crucial mechanism in plant development. We have shown that Arabidopsis F-box protein TIR1 receptor for auxin, and our recent structural work has revealed molecular perception. substrate ubiquitin–ligase complex SCF . Auxin binding enhances interaction between its substrates, Aux/IAA repressors, thereby promoting ubiquitination degradation Aux/IAAs, altering hundreds genes. prototype new class first example an ubiquitin-ligase...
ent ‐Kaurene is the key intermediate in biosynthesis of gibberellins (GAs), plant hormones. In higher plants, ‐kaurene synthesized successively by copalyl diphosphate synthase (CPS) and (KS) from geranylgeranyl (GGDP). On other hand, fungal synthases are bifunctional cyclases with both CPS KS activity a single polypeptide. The moss Physcomitrella patens model organism for study genetics development an early land plant. We identified (PpCPS/KS) P. analyzed its function. PpCPS/KS cDNA encodes...
Summary Indole‐3–acetic acid ( IAA ), an auxin plant hormone, is biosynthesized from tryptophan. The indole‐3–pyruvic IP y A ) pathway, involving the tryptophan aminotransferase TAA 1 and YUCCA YUC enzymes, was recently found to be a major biosynthetic pathway in Arabidopsis. catalyzes conversion of , produces . Using chemical biology approach with maize coleoptiles, we identified 5–(4–chlorophenyl)‐4H‐1,2,4–triazole‐3–thiol (yucasin) as potent inhibitor biosynthesis ‐expressing coleoptile...
The phytohormone auxin plays a central role in many aspects of plant growth and development. IAA is the most studied natural that possesses property polar transport plants. Phenylacetic acid (PAA) has also been recognized as for >40 years, but its development remains unclear. In this study, we show PAA have overlapping regulatory roles distinct characteristics auxins widely distributed vascular non-vascular Although biological activities are lower than those IAA, endogenous levels much...
The fruit of melting-flesh peach (Prunus persica L. Batsch) cultivars produce high levels ethylene caused by expression PpACS1 (an isogene 1-aminocyclopropane-1-carboxylic acid synthase), resulting in rapid softening at the late-ripening stage. In contrast, stony hard do not soften and little due to low PpACS1. To elucidate mechanism for suppressing peaches, a microarray analysis was performed. Several genes that displayed similar patterns as were identified shown be indole-3-acetic...
Controlling protein expression using a degron is advantageous because the of interest can be rapidly depleted in reversible manner. We pioneered development auxin-inducible (AID) technology by transplanting plant-specific degradation pathway to non-plant cells. In human cells expressing an E3 ligase component, OsTIR1, it possible degrade degron-fused with half-life 15-45 min presence phytohormone auxin. reported previously generation HCT116 mutants which C terminus endogenous proteins was...
Momilactones are bioactive diterpenoids that contribute to plant defense against pathogens and allelopathic interactions between plants. Both cultivated wild grass species of Oryza Echinochloa crus-galli (barnyard grass) produce momilactones using a biosynthetic gene cluster (BGC) in their genomes. The bryophyte Calohypnum plumiforme (formerly Hypnum plumaeforme) also produces momilactones, the bifunctional diterpene cyclase CpDTC1/HpDTC1, which is responsible for production framework, has...
Abstract p-Chlorophenoxyisobutyric acid (PCIB) is known as a putative antiauxin and widely used to inhibit auxin action, although the mechanism of PCIB-mediated inhibition action not characterized very well at molecular level. In present work, we showed that PCIB inhibited BA::β-glucuronidase (GUS) expression induced by indole-3-acetic (IAA), 2,4-dichlorophenoxyacetic acid, 1-naphthaleneacetic acid. also auxin-dependent DR5::GUS expression. RNA hybridization quantitative reverse...
Gibberellins (GAs) are a group of diterpene-type plant hormones biosynthesized from ent-kaurene via ent-kaurenoic acid. GAs ubiquitously present in seed plants. The GA signal is perceived and transduced by the GID1 receptor/DELLA repressor pathway. lycopod Selaginella moellendorffii biosynthesizes has functional GID1-DELLA signaling components. In contrast, no or functionally orthologous components have been found moss Physcomitrella patens. However, P. patens produces ent-kaurene, common...
IAA is a naturally occurring auxin that plays crucial role in the regulation of plant growth and development. The endogenous concentration spatiotemporally regulated by biosynthesis, transport its inactivation plants. Previous studies have shown metabolism to 2-oxindole-3-acetic acid (OxIAA) OxIAA-glucoside (OxIAA-Glc) may play an important homeostasis, but genes involved this metabolic pathway are still unknown. In study, we show UGT74D1 catalyzes glucosylation OxIAA Arabidopsis. By...
Significance Fluorescent auxin analogs are designed to function as active auxins for the transport system but be inactive signaling. These fluorescent can mimic via and used visualize inter- intracellular distribution in roots. allow imaging of sites with high spatiotemporal resolution. Our provides insight into dynamics subcellular distribution.
Auxin is the first discovered plant hormone and essential for many aspects of growth development. Indole-3-acetic acid (IAA) main auxin plays pivotal roles in intercellular communication through polar transport. Phenylacetic (PAA) another natural that does not show movement. Although a wide range species have been shown to produce PAA, its biosynthesis, inactivation physiological significance plants are largely unknown. In this study, we demonstrate overexpression CYP79A2 gene, which...
The phytohormone auxin, indole-3-acetic acid (IAA), plays a prominent role in plant development. Auxin homeostasis is coordinately regulated by auxin synthesis, transport, and inactivation; however, the physiological contribution of inactivation to has not been determined. GH3 IAA–amino conjugating enzymes play central inactivation. Chemical inhibition proteins planta challenging because these leads IAA overaccumulation that rapidly induces expression. Here, we report characterization potent...
A (polymer network/nematic liquid crystal/chiral dopant) composite exhibiting a chiral nematic (N*) phase at room temperature has been developed. Because the helical twisting power of dopant increases with increasing and polymer network affects molecular rearrangement crystal, bandwidth selective reflection spectrum N* becomes wider narrower reversibly decreasing temperature, respectively.
We examined whether auxin/indole-3-acetic acid (Aux/IAA) proteins, which are key players in auxin-signal transduction, involved brassinosteroid (BR) responses. iaa7/axr2-1 and iaa17/axr3-3 mutants showed aberrant BR sensitivity BR-induced gene expression an organ-dependent manner. Two auxin inhibitors were tested terms of Yokonolide B inhibited responses, whereas p-chlorophenoxyisobutyric did not inhibit DNA microarray analysis revealed that 108 genes up-regulated, while only eight...