Abstract The plant hormones auxin and ethylene have been shown to play important roles during root hair development. However, cross talk between makes it difficult understand the independent role of either hormone. To dissect their respective roles, we examined effects two compounds, chromosaponin I (CSI) 1-naphthoxyacetic acid (1-NOA), on developmental process in wild-type Arabidopsis, ethylene-insensitive mutantein2-1, influx mutants aux1-7,aux1-22, double mutant aux1-7 ein2....

Abstract p-Chlorophenoxyisobutyric acid (PCIB) is known as a putative antiauxin and widely used to inhibit auxin action, although the mechanism of PCIB-mediated inhibition action not characterized very well at molecular level. In present work, we showed that PCIB inhibited BA::β-glucuronidase (GUS) expression induced by indole-3-acetic (IAA), 2,4-dichlorophenoxyacetic acid, 1-naphthaleneacetic acid. also auxin-dependent DR5::GUS expression. RNA hybridization quantitative reverse...

Gamma rays are the most frequently used ionizing radiation in plant mutagenesis; however, few studies available on characteristics of mutations at a genome-wide level. Here, we quantitatively and qualitatively characterized induced by acute/chronic gamma ray irradiation Arabidopsis. The data were then compared with those previously obtained for carbon ion irradiation. In acute dry seeds same effective survival dose, ions differed substantially, former inducing significantly greater number...

Abstract Light/dark cycling is an inherent condition of outdoor microalgae cultivation, but often unfavorable for lipid accumulation. This study aims to identify promising targets metabolic engineering improved accumulation under conditions. Consequently, the lipid-rich mutant Chlamydomonas sp. KOR1 was developed through light/dark-conditioned screening. During dark periods with depressed CO 2 fixation, shows rapid carbohydrate degradation together increased and carotenoid contents....

An Arabidopsis transgenic line was constructed expressing β-glucuronidase (GUS) via the auxin-responsive domains (AuxRDs) A and B (BA–GUS) of PS-IAA4/5 gene in an indoleacetic acid (IAA)–dependent fashion. GUS expression preferentially enhanced root elongation zone after treatment young seedlings with 10−7 M IAA. Expression BA–GUS axr1, axr4, aux1 mutants required 10- to 100-fold higher auxin concentration than that wild-type background. nil axr2 axr3 mutants. The transgene used isolate...

Abstract Ionizing radiation including heavy-ion beams has been widely used in mutation breeding. Dry seeds, seedlings, and cultured tissues are often for mutagenesis; however, little is known about the differences induced mutations among them. Here, we examined characteristics of using randomly chosen Arabidopsis M 2 plants derived from dry seeds seedlings irradiated with carbon ions. The frequency was 1.4–1.9 times higher dry-seed irradiation than seedling irradiation. This difference...

Abstract We investigated the role of ethylene and auxin in regulating growth morphology roots during mechanical impedance by developing a new growing system using model plant Arabidopsis (Arabidopsis thaliana). The seedlings grown horizontally on dialysis membrane-covered agar plate encountered adequate as showed characteristic phenotypes: 2-fold reduction root growth, increase diameter, decrease cell elongation, ectopic hair formation. phenotype characterization various mutants having...

A DNA fragment possessing ORF12 of TL-DNA Ri(A4) plasmids (Slightom et al. 1986) was inserted into the genome Nicotiana tabacum cv. Petite Havana SR1, through binary vector system harbored by Agrobacterium tumefaciens. The transformed plants showed dwarfness in plant height and corolla length. Adventitious roots were not induced from leaf disks A. tumefaciens ORF12.

Abstract To isolate novel auxin-responsive mutants in Arabidopsis (Arabidopsis thaliana), we screened for root growth resistance to a putative antiauxin, p-chlorophenoxyisobutyric acid (PCIB), which inhibits auxin action by interfering the upstream auxin-signaling events. Eleven PCIB-resistant were obtained. Genetic mapping indicates that mutations are located at least five independent loci, including two known auxin-related TRANSPORT INHIBITOR RESPONSE1 and CULLIN1. antiauxin-resistant...

Radiation-induced mutations have been detected by whole-genome sequencing analyses of self-pollinated generations mutagenized plants. However, large DNA alterations and in non-germline cells were likely missed. In this study, order to detect various types M1 plants, anthocyanin pigmentation was used as a visible marker mutations. Arabidopsis seeds heterozygous for the biosynthetic genes irradiated with gamma-rays. Anthocyanin-less vegetative sectors resulting from loss heterozygosity...

Summary 2,4‐dichlorophenoxyacetic acid (2,4‐D), a chemical analogue of indole‐3‐acetic (IAA), is widely used as growth regulator and exogenous source auxin. Because 2,4‐D evokes physiological molecular responses similar to those evoked by IAA, it believed that they share common response pathway. Here, we show mutant, antiauxin resistant1 ( aar1 ), identified in screen for resistance the anti‐auxin p ‐chlorophenoxy‐isobutyric (PCIB), resistant 2,4‐D, yet nevertheless responds like wild‐type...

Mutation-breeding technologies are useful tools for the development of new biological resources in plants and microorganisms. In Takasaki Ion Accelerators Advanced Radiation Application (TIARA) at National Institutes Quantum Radiological Science Technology, Japan, ion beams were explored as novel mutagens. The mutagenic effects various on eukaryotic prokaryotic microorganisms described their application breeding technology industrial discussed. Generally, relative effectiveness (RBE)...

Streptomyces lividans TK23 interacts with mycolic acid-containing bacteria (MACB), such as Tsukamurella pulmonis TP-B0596, and this direct cell contact activates its secondary metabolism (e.g., the production of undecylprodigiosin: RED). Here, we employed carbon ( 12 C 5+ ) ion beam-induced mutagenesis to investigate signature induced point mutations further identify gene(s) responsible for metabolites by T . We irradiated spores coelicolor strain JCM4020 ions generate a mutant library....

Progeny plants obtained by self-crossing transgenic tobacco possessing the rolC gene (ORF 12 of TL-DNA Ri plasmid) showed stable inheritance dwarfness in plant height as well floral organs. A histological analysis dwarf indicated that shortening internodal length was associated with a smaller epidermal cells. Furthermore, expressing characters earlier flowering than normal one. The organ-specific transcription independent influence other genes.

2,4-Dichlorophenoxyacetic acid (2,4-D), a functional analogue of auxin, is used as an exogenous source auxin it evokes physiological responses like the endogenous indole-3-acetic (IAA). Previous molecular analyses response pathway revealed that IAA and 2,4-D share common mode action to elicit downstream responses. However, recent findings with 2,4-D-specific mutants suggested might also use distinct pathways modulate root growth in Arabidopsis. Using genetic cellular approaches, we...

Abstract Previously, a dysfunction of the SMALL ACIDIC PROTEIN1 (SMAP1) gene was identified as cause anti-auxin resistant1 (aar1) mutant Arabidopsis (Arabidopsis thaliana). SMAP1 is involved in response pathway synthetic auxin, 2,4-dichlorophenoxyacetic acid, and functions upstream auxin/indole-3-acetic acid protein degradation step auxin signaling. However, exact mechanism by which signaling remains unknown. Here, we demonstrate that required for normal plant growth development root to...