A5082113683- Plant Molecular Biology Research
- Plant Stress Responses and Tolerance
- Plant Reproductive Biology
- Photosynthetic Processes and Mechanisms
- Plant nutrient uptake and metabolism
University of California, San Diego
2021-2022
Nanjing Agricultural University
2021
Abstract Inactivation of the phytohormone auxin plays important roles in plant development, and several enzymes have been implicated inactivation. In this study, we show that predominant natural auxin, indole-3-acetic acid (IAA), is mainly inactivated via GH3-ILR1-DAO pathway. IAA first converted to IAA-amino conjugates by GH3 IAA-amidosynthetases. The IAA-aspartate (IAA-Asp) IAA-glutamate (IAA-Glu) are storage forms can be back ILR1/ILL amidohydrolases. We further DAO1 dioxygenase...
Gretchen Hagen 3 (GH3) amido synthetases conjugate amino acids to a carboxyl group of small molecules including hormones auxin, jasmonate, and salicylic acid. The Arabidopsis genome harbors 19 GH3 genes, whose exact roles in plant development have been difficult define because genetic redundancy among the genes. Here we use CRISPR/Cas9 gene editing technology delete II which are able indole-3-acetic acid (IAA) acids. We show that plants lacking eight genes (gh3 octuple mutants) accumulate...
Auxin inactivation is critical for plant growth and development. To develop regulators functioning in auxin pathway, we performed a phenotype-based chemical screen Arabidopsis identified chemical, nalacin, that partially mimicked the effects of auxin. Genetic, pharmacological, biochemical approaches demonstrated nalacin exerts its auxin-like activities by inhibiting indole-3-acetic acid (IAA) conjugation mediated Gretchen Hagen 3 (GH3) acyl amido synthetases. The crystal structure GH3.6...
Abstract Gretchen Hagen 3 (GH3) amido synthetases conjugate amino acids to a carboxyl group of small molecules including hormones auxin, jasmonate, and salicylic acid. The Arabidopsis genome harbors 19 GH3 genes, whose exact roles in plant development have been difficult define because genetic redundancy among the genes. Here we use CRISPR/Cas9 gene editing technology delete II which are able indole-3-acetic acid (IAA) acids. We show that plants lacking eight genes ( gh3 octuple mutants)...
Abstract Root system is critical for plant growth and development. To develop regulators functioning in root development, we performed a phenotype-based chemical screen Arabidopsis identified chemical, nalacin, that mimicked the effects of auxin on Genetic, pharmacological biochemical approaches demonstrated nalacin exerts its auxin-like activities by inhibiting indole-3-acetic acid (IAA) conjugation mediated Gretchen Hagen 3 (GH3) acyl amido synthetases. The crystal structure GH3.6 complex...