A5101639620- Plant Molecular Biology Research
- Genomic variations and chromosomal abnormalities
- Genomics and Rare Diseases
- Genetics and Neurodevelopmental Disorders
- Rice Cultivation and Yield Improvement
- Food composition and properties
- Plant Stress Responses and Tolerance
- GABA and Rice Research
- Genomics and Phylogenetic Studies
- Plant-Microbe Interactions and Immunity
- Chromosomal and Genetic Variations
- Cleft Lip and Palate Research
- Plant tissue culture and regeneration
- Plant nutrient uptake and metabolism
- Plant Reproductive Biology
- Craniofacial Disorders and Treatments
- Plant Parasitism and Resistance
- Plant Gene Expression Analysis
- Plant Genetic and Mutation Studies
- Plant responses to elevated CO2
- Photosynthetic Processes and Mechanisms
- Circadian rhythm and melatonin
- Cancer Genomics and Diagnostics
- Plant biochemistry and biosynthesis
- Genetic Mapping and Diversity in Plants and Animals
ZheJiang Academy of Agricultural Sciences
2013-2025
Prince of Wales Hospital
2018-2024
Hunter Genetics
2015-2024
Jiaxing University
2024
Neuroscience Research Australia
2021-2024
Ministry of Agriculture and Rural Affairs
2023
Peking University First Hospital
2019-2022
Peking University
2013-2022
Southern University of Science and Technology
2019-2022
State Forestry and Grassland Administration
2022
Abstract Salicylic acid (SA) plays a central role in plant disease resistance, and emerging evidence indicates that auxin, an essential hormone regulating growth development, is involved susceptibility. GH3.5, member of the GH3 family early auxin-responsive genes Arabidopsis (Arabidopsis thaliana), encodes protein possessing vitro adenylation activity on both indole-3-acetic (IAA) SA. Here, we show GH3.5 acts as bifunctional modulator SA auxin signaling during pathogen infection....
An elevated growth temperature often inhibits plant defense responses and renders plants more susceptible to pathogens. However, the molecular mechanisms underlying this modulation are unknown. To genetically dissect regulation, we isolated mutants that retain disease resistance at a higher in Arabidopsis. One such heat-stable mutant results from point mutation SNC1, NB-LRR encoding gene similar (R) genes. Similar mutations introduced into tobacco R gene, N, confer temperature. Thus genes or...
Plant-pathogen interactions are known to be affected by environmental factors including temperature; however, the temperature effects have not been systematically studied in plant disease resistance. Here, we characterized of a moderate increase on resistance bacterial pathogen Pseudomonas syringae and two viral elicitors Arabidopsis thaliana Nicotiana benthamiana. Both basal (R) gene-mediated defense responses found inhibited moderately high temperature, hypersensitive induced R genes...
Starch composition and the amount in endosperm, both of which contribute dramatically to seed yield, cooking quality, taste cereals, are determined by a series complex biochemical reactions. However, mechanism regulating starch biosynthesis cereal seeds is not well understood. This study showed that OsbZIP58, bZIP transcription factor, key transcriptional regulator controlling synthesis rice endosperm. OsbZIP58 was expressed mainly endosperm during active synthesis. osbzip58 null mutants...
<h3>Importance</h3> Widespread adoption of rapid genomic testing in pediatric critical care requires robust clinical and laboratory pathways that provide equitable consistent service across health systems. <h3>Objective</h3> To prospectively evaluate the performance a multicenter network for ultra-rapid diagnosis public system. <h3>Design, Setting, Participants</h3> Descriptive feasibility study critically ill patients with suspected monogenic conditions treated at 12 Australian hospitals...
Abstract Whole genome sequencing (WGS) improves Mendelian disorder diagnosis over whole exome (WES); however, additional diagnostic yields and costs remain undefined. We investigated differences between cost outcomes of WGS WES in a cohort with suspected disorders. was performed 38 WES-negative families derived from 64 family that previously underwent WES. For new diagnoses, contemporary reanalysis determined whether variants were diagnosable by original or unique to WGS. Diagnostic rates...
Disease resistance (R) proteins, as central regulators of plant immunity, are tightly regulated for effective defense responses and to prevent constitutive activation under non-pathogenic conditions. Here we report the identification an F-box protein CPR1/CPR30 a negative regulator R SNC1 likely through SCF (Skp1-cullin-F-box) mediated degradation. The cpr1-2 (cpr30-1) loss-of-function mutant has responses, it interacts synergistically with gain-of function snc1-1 bon1-1 where is...
Plant defense responses to pathogens are influenced by abiotic factors, including temperature. Elevated temperatures often inhibit the activities of disease resistance proteins and they mediate. A mutant screen with an Arabidopsis thaliana temperature-sensitive autoimmune bonzai1 revealed that abscisic acid (ABA)–deficient aba2 enhances mediated (R) gene SUPPRESSOR OF npr1-1 CONSTITUTIVE1 (SNC1) at high ABA deficiency promoted nuclear accumulation SNC1, which was essential for it function...
Abstract Background Epileptic encephalopathies are a devastating group of neurological conditions in which etiological diagnosis can alter management and clinical outcome. Exome sequencing gene panel testing improve diagnostic yield but there is no cost‐effectiveness analysis their use or consensus on how to best integrate these tests into pathways. Methods We conducted retrospective study comparing trio exome with standard approach, for well‐phenotyped cohort 32 patients epileptic...
Previous studies have profiled breast cancer compared to normal tissue and identified differentially expressed microRNAs (miRNAs). These miRNAs are then assessed in serum of patients healthy volunteers. MiRNAs however do not always reflect what is important may be missed. PCR arrays were therefore performed on samples from volunteers with the aim identifying circulating that more highly early controls. Taqman low density array (TLDA) cards used profile a discovery cohort 39 10 The results...
Abstract High temperature (HT) has an adverse effect on rice grain filling by inhibiting the accumulation of storage materials. However, regulatory mechanism this inhibition remains unknown. Here, we report that Opaque2 like transcription factor OsbZIP58 is a key regulating material under HT. The gene promotes expression many seed protein genes and starch synthesis while inhibits some hydrolyzing α‐amylase loss function leads to floury shrunken endosperms dramatically reduced materials in...
Summary High temperature significantly alters the amylose content of rice, resulting in mature grains with poor eating quality. However, only few genes and/or quantitative trait loci involved this process have been isolated and molecular mechanisms effect remain unclear. Here, we describe a floral organ identity gene, Os MADS 7 , stabilizing rice at high temperature. is greatly induced by early filling stage. Constitutive suppression stabilizes under stress but results low spikelet...
Abstract Melatonin ( N ‐acetyl‐5‐methoxytryptamine) plays important roles in plant defences against a variety of biotic and abiotic stresses, including UV‐B stress. Molecular mechanisms underlying functions melatonin responses are poorly understood. Here, we show that effect on molecular signalling pathways, physiological changes stress resistance Arabidopsis . Both exogenous endogenous affected expression signal transduction pathway genes. Experiments using component mutants cop1‐4 hy5‐215...
Background Recent advances in genomics methodologies, particular the availability of next‐generation sequencing approaches have made it possible to identify risk loci throughout genome, exome. In current study, we present findings from an exome study conducted five affected individuals a multiplex family with cleft palate only. Methods The GEnome MINIng (GEMINI) pipeline was used functionally annotate single nucleotide polymorphisms, insertions and deletions. Filtering methods were applied...
Melatonin is an important bioactive molecule in plants. Two synthetases, N-acetylserotonin methyltransferase (ASMT) and serotonin N-acetyltransferase (SNAT) are involved the final two steps of melatonin synthesis. participates responses to a variety biotic abiotic stresses plants, but few studies have addressed roles endogenous pathogen resistance. We investigated role resistance Botrytis cinerea infection Arabidopsis thaliana model system. Plant lines that overexpressed ASMT or SNAT through...
Summary The phytohormones abscisic acid (ABA) and gibberellic (GA) antagonistically control the shift between seed dormancy its alleviation. DELAY OF GERMINATION1 (DOG1) is a critical regulator that determines intensity of primary dormancy, but underlying regulatory mechanism unclear. In this study, we combined physiological, biochemical, genetic approaches to reveal bHLH transcriptional factor WRKY36 progressively silenced DOG1 expression break through ABI5‐BINDING PROTEIN 2 (AFP2) as...
Seed germination is a complex, multistage developmental process that an important step in plant development. In this study, RNA-Seq was conducted the embryo and endosperm of unshelled germinating rice seeds. A total 14,391 differentially expressed genes (DEGs) were identified between dry seeds Of these DEGs, 7109 both endosperm, 3953 specific, 3329 specific. The embryo-specific DEGs enriched plant-hormone signal-transduction pathway, while endosperm-specific phenylalanine, tyrosine,...