A5072700228- Plant Molecular Biology Research
- Plant Gene Expression Analysis
- Plant Stress Responses and Tolerance
- Plant Reproductive Biology
- Plant Physiology and Cultivation Studies
- Horticultural and Viticultural Research
- Postharvest Quality and Shelf Life Management
- Plant biochemistry and biosynthesis
- Cancer Genomics and Diagnostics
- Light effects on plants
- Cancer Immunotherapy and Biomarkers
- Plant tissue culture and regeneration
- Plant responses to water stress
- Plant Virus Research Studies
- RNA modifications and cancer
- Polysaccharides and Plant Cell Walls
- Metabolism and Genetic Disorders
- Biochemical and biochemical processes
- Epigenetics and DNA Methylation
- Lung Cancer Treatments and Mutations
- Plant Disease Management Techniques
- Plant nutrient uptake and metabolism
- Antioxidant Activity and Oxidative Stress
- Plant Surface Properties and Treatments
- Peroxisome Proliferator-Activated Receptors
Apple (Israel)
2024
Northwest A&F University
2014-2024
Southern University of Science and Technology
2019-2024
Zero to Three
2024
Planta
2024
Institute of Plant Biology
2024
Wuhan University
2020-2022
Summary Apple ( Malus × domestica ) trees are vulnerable to freezing temperatures. However, there has been only limited success in developing cold‐hardy cultivars. This lack of progress is due at least partly understanding the molecular mechanisms tolerance apple. In this study, we evaluated potential roles for two R2R3 MYB transcription factors TF s), 88 and paralogous FLP 124), cold stress apple Arabidopsis . We found that 124 positively regulate cold‐responsive gene expression both...
Water deficit is one of the main limiting factors in apple (Malus × domestica Borkh.) cultivation. Root architecture plays an important role drought tolerance plants; however, research efforts to improve trees have focused on aboveground targets. Due difficulties associated with visualization and data analysis, there currently a poor understanding genetic players molecular mechanisms involved root under conditions. We previously observed that MdMYB88 its paralog MdMYB124 regulate tree...
MdMYB88 and MdMYB124 have been demonstrated to be responsible for lignin accumulation in apple under drought stress. In this study, using a metabolomic approach, we identified differentially accumulated phenylpropanoid flavonoid metabolites MdMYB88/124 transgenic RNAi plants control long-term stress conditions roots. We confirmed the regulation of phenylalanine by via UPLC-MS roots both conditions. Using Electrophoretic Mobility Shift Assay (EMSA) ChIP-quantitative PCR (qPCR) analyses, found...
Despite the relationship between melatonin and aging, overall changes regulation of proteome profiling by long-term exposure during leaf senescence is not well understood. In this study, in Malus hupehensis plants was delayed when exogenous regularly applied to roots for 2 months compared with natural senescence. Proteins samples 0 50 day both treatments were extracted labeled TMT regents before being examined via NanoLC-MS/MS. The proteomics data showed that 622 309 proteins altered...
Drought stress can negatively impact apple fruit quality and yield. Apple microRNAs (miRNAs) participate in tree development, as well biotic tolerance; however, it is largely unknown whether these molecules are involved the drought response. To identify drought-responsive miRNAs Malus, we first examined tolerance of ten F1 progenies R3 (M. × domestica) M. sieversii. We performed Illumina sequencing on pooled total RNA from both drought-tolerant drought-sensitive plants. The results...
Abstract Background Domesticated apple (Malus × domestica Borkh) is a popular temperate fruit with high nutrient levels and diverse flavors. In 2012, global production accounted for at least one tenth of all harvested fruits. A high-quality genome assembly crucial the selection breeding new cultivars. Currently, single reference available apple, assembled from 16.9 coverage short reads via Sanger 454 sequencing technologies. Although useful resource, this covers only ~89 % non-repetitive...
Abstract The evolutionary history of the Malus genus has not been well studied. In current study, we presented genetic evidence on origin based genome sequencing 297 accessions, revealing relationship between wild species and cultivated apples. Our results demonstrated that North American East Asian are closer to outgroup (pear) than Central species, hybrid including natural (separated before Pleistocene, about 2.5 Mya) artificial hybrids (including ornamental trees rootstocks) species....
The R2R3 transcription factor MdMYB88 has previously been reported to function in biotic and abiotic stress responses. Here, we identify BRI1 ETHYLMETHANE SULFONATE SUPRESSOR1 (MdBES1), a vital component of brassinosteroid (BR) signaling apple (Malus × domestica) that directly binds the promoter, regulating expression dynamic multifaceted mode. MdBES1 positively regulated under cold pathogen attack, but negatively its control drought conditions. Consistently, was positive regulator for...
Abstract Apple (Malus domestica) trees often experience various abiotic and biotic stresses. However, due to the long juvenile period of apple its high degree genetic heterozygosity, only limited progress has been made in developing cold-hardy disease-resistant cultivars through traditional approaches. Numerous studies reveal that biotechnology is a feasible approach improve stress tolerance woody perennial plants. HYPONASTIC LEAVES1 (HYL1), double-stranded RNA-binding protein, key regulator...
Abstract The phytohormone abscisic acid (ABA) is involved in various plant processes. In response to drought stress, plants quickly accumulate ABA, but the regulatory mechanism of ABA accumulation largely unknown, especially woody plants. this study, we report that MdMYB88 and MdMYB124 are myeloblastosis (MYB) transcription factors critical for apple trees (Malus x domestica) following drought, regulation negatively controlled by ABA. positively regulate leaf water transpiration,...
Grafting, an ancient horticultural cultivation technique, is essential for commercial apple (Malus × domestica Borkh.) production. M. prunifolia 'Fupingqiuzi' used as a common rootstock due to its known tolerances abiotic and biotic stresses. However, the genetic origin evolutionary history of are largely unknown, molecular bases underlying innate stress tolerance characteristics ability graft-transmit scion. Here, we present high-quality chromosome-scale genome sequence (2n = 2x 34)...
The function of serrate (SE) in miRNA biogenesis Arabidopsis is well elucidated, whereas its role plant drought resistance largely unknown. In this study, we report that MdSE acts as a negative regulator apple (Malus × domestica) by regulating the expression levels MdMYB88 and MdMYB124 miRNAs, including mdm-miR156, mdm-miR166, mdm-miR172, mdm-miR319, mdm-miR399. interacts with MdMYB124, two positive regulators resistance. decreases transcript protein which directly regulate MdNCED3, key...
Auxin inactivation is critical for plant growth and development. To develop regulators functioning in auxin pathway, we performed a phenotype-based chemical screen Arabidopsis identified chemical, nalacin, that partially mimicked the effects of auxin. Genetic, pharmacological, biochemical approaches demonstrated nalacin exerts its auxin-like activities by inhibiting indole-3-acetic acid (IAA) conjugation mediated Gretchen Hagen 3 (GH3) acyl amido synthetases. The crystal structure GH3.6...
Plant development is regulated by both synergistic and antagonistic interactions of different phytohormones, including a complex crosstalk between ethylene auxin. For instance, auxin synergistically control primary root elongation hair formation. However, lack chemical agents that specifically modulate or production has precluded precise delineation the contribution each hormone to development. Here, we performed genetic screen based on recovery growth in ethylene-related Arabidopsis mutants...
Freezing stress is a major environmental factor that threatens the growth and development of fruit trees. MdMYB88 its paralogue MdMYB124 have been identified as pivotal regulators in apple (Malus × domestica) freezing tolerance. Here, we demonstrated target MdMYB124, TIME FOR COFFEE (TIC), contributes to tolerance apple. directly bound MdTIC promoter positively regulated expression under cold conditions. RNAi plants displayed reduced when was repressed. Moreover, lowered antioxidant enzyme...
Abstract The apical hook is indispensable for protecting the delicate shoot meristem while dicot seedlings emerge from soil after germination in darkness. development of co-ordinately regulated by multiple phytohormones and environmental factors. Yet, a holistic understanding spatial–temporal interactions between different factors remains to be achieved. Using chemical genetic approach, we identified kinetin riboside, as proxy kinetin, which promotes Arabidopsis thaliana partially...
ABSTRACT Anchorene, identified as an endogenous bioactive carotenoid‐derived dialdehyde and diapocarotenoid, affects root development by modulating auxin homeostasis. However, the precise interaction between anchorene auxin, well mechanisms which modulates levels, remain largely elusive. In this study, we conducted a comparative analysis of anchorene's bioactivities alongside observed that induces multifaceted auxin‐like effects. Through genetic pharmacological examinations, revealed...
Introduction: Recent studies exhibited the unstable prediction ability of blood-based tumor mutational burden (bTMB) when predicting response immune checkpoint inhibitors (ICIs) therapy in patients with non-small cell lung cancer (NSCLC). Circulating DNA (ctDNA) abundance, usually represented by maximum somatic allele frequency (MSAF), was one possible confounding factor influencing bTMB ICIs prediction. Methods: MSAF-adjusted (Ma-bTMB) established and validated advanced NSCLC among Geneplus...
The apical hook is indispensable for protecting the delicate shoot meristem while dicot seedlings emerging from soil after germination in darkness. development of coordinately regulated by multiple phytohormones and environmental factors. Yet, a holistic understanding spatial-temporal interactions between different factors remains to be achieved. By chemical genetics method, we identified kinetin riboside, as proxy kinetin, that promotes Arabidopsis thaliana partially ethylene-signaling...
<title>Abstract</title> Acetophenones, which show scattered distribution in organisms including phylogenetically distant plants and fungi, are play diverse roles plant-plant, plant-insect, plant-microbiome, even animal-insect interactions. However, the enzymes involved plant acetophenone biosynthetic pathway remain enigmatic. Here we uncover complete biosynthesis of 4-hydroxyacetophenone glucoside using pear as a model organism. We demonstrate that natural occurrence certain cultivars is due...