A5101620815- Plant Molecular Biology Research
- Plant Stress Responses and Tolerance
- Plant Gene Expression Analysis
- Plant biochemistry and biosynthesis
- Plant Pathogens and Fungal Diseases
- Plant Physiology and Cultivation Studies
- Land Use and Ecosystem Services
- Horticultural and Viticultural Research
- Plant responses to water stress
- Fungal Plant Pathogen Control
- Postharvest Quality and Shelf Life Management
- Polysaccharides and Plant Cell Walls
- Remote Sensing and Land Use
- Phytochemistry and Biological Activities
- Plant-Microbe Interactions and Immunity
- Photosynthetic Processes and Mechanisms
- Plant Reproductive Biology
- Remote Sensing in Agriculture
- Medicinal Plant Extracts Effects
- Cocoa and Sweet Potato Agronomy
- Phytochemical and Pharmacological Studies
- Biosensors and Analytical Detection
- Food Quality and Safety Studies
- Insect-Plant Interactions and Control
- Plant Genetic and Mutation Studies
Northwest A&F University
2017-2025
Zhejiang Chinese Medical University
2020-2025
China Design Group (China)
2024
Jinzhou Medical University
2023
University of Connecticut
2020
Jinan University
2019
Shanghai Zhangjiang Laboratory
2014
Huazhong Agricultural University
2012
Shanghai Pesticide Research Institute
2009
Zhejiang University
2007
Summary Apple ( Malus × domestica ) trees are vulnerable to freezing temperatures. However, there has been only limited success in developing cold‐hardy cultivars. This lack of progress is due at least partly understanding the molecular mechanisms tolerance apple. In this study, we evaluated potential roles for two R2R3 MYB transcription factors TF s), 88 and paralogous FLP 124), cold stress apple Arabidopsis . We found that 124 positively regulate cold‐responsive gene expression both...
Water deficit is one of the main limiting factors in apple (Malus × domestica Borkh.) cultivation. Root architecture plays an important role drought tolerance plants; however, research efforts to improve trees have focused on aboveground targets. Due difficulties associated with visualization and data analysis, there currently a poor understanding genetic players molecular mechanisms involved root under conditions. We previously observed that MdMYB88 its paralog MdMYB124 regulate tree...
MdMYB88 and MdMYB124 have been demonstrated to be responsible for lignin accumulation in apple under drought stress. In this study, using a metabolomic approach, we identified differentially accumulated phenylpropanoid flavonoid metabolites MdMYB88/124 transgenic RNAi plants control long-term stress conditions roots. We confirmed the regulation of phenylalanine by via UPLC-MS roots both conditions. Using Electrophoretic Mobility Shift Assay (EMSA) ChIP-quantitative PCR (qPCR) analyses, found...
Drought stress can negatively impact apple fruit quality and yield. Apple microRNAs (miRNAs) participate in tree development, as well biotic tolerance; however, it is largely unknown whether these molecules are involved the drought response. To identify drought-responsive miRNAs Malus, we first examined tolerance of ten F1 progenies R3 (M. × domestica) M. sieversii. We performed Illumina sequencing on pooled total RNA from both drought-tolerant drought-sensitive plants. The results...
Abstract Drought stress tolerance is a complex trait regulated by multiple factors. Here, we demonstrate that the miRNA160–Auxin Response Factor 17 (ARF17)–HYPONASTIC LEAVES 1 module crucial for apple (Malus domestica) drought tolerance. Using stable transgenic plants, found was improved higher levels of Mdm-miR160 or MdHYL1 and decreased MdARF17, whereas reductions in increases MdARF17 led to greater sensitivity. Further study revealed modulation achieved through regulation...
SUMMARY Drought significantly limits apple fruit production and quality. Decoding the key genes involved in drought stress tolerance is important for breeding varieties with improved resistance. Here, we identified GRETCHEN HAGEN3.6 (GH3.6), an indole‐3‐acetic acid (IAA) conjugating enzyme, to be a negative regulator of water‐deficit apple. Overexpressing MdGH3 . 6 reduced IAA content, adventitious root number, length tolerance, whereas knocking down MdGH3.6 its close paralogs increased...
SUMMARY Apple ( Malus domestica ) trees are vulnerable to freezing temperatures. Cold resistance in woody perennial plants can be improved through biotechnological approaches. However, genetic engineering requires a thorough understanding of the molecular mechanisms tree's response cold. In this study, we demonstrated that Mdm‐miR160–MdARF17–MdWRKY33 module is crucial for apple tolerance. Mdm‐miR160 plays negative role tolerance, whereas MdARF17, one targets Mdm‐miR160, positive regulator...
Abstract The evolutionary history of the Malus genus has not been well studied. In current study, we presented genetic evidence on origin based genome sequencing 297 accessions, revealing relationship between wild species and cultivated apples. Our results demonstrated that North American East Asian are closer to outgroup (pear) than Central species, hybrid including natural (separated before Pleistocene, about 2.5 Mya) artificial hybrids (including ornamental trees rootstocks) species....
Abstract Apple (Malus domestica) trees often experience various abiotic and biotic stresses. However, due to the long juvenile period of apple its high degree genetic heterozygosity, only limited progress has been made in developing cold-hardy disease-resistant cultivars through traditional approaches. Numerous studies reveal that biotechnology is a feasible approach improve stress tolerance woody perennial plants. HYPONASTIC LEAVES1 (HYL1), double-stranded RNA-binding protein, key regulator...
Abstract The Diospyros genus , which includes both wild and cultivated species such as lotus kaki, represents a diverse genetic pool with significant agricultural value. In this study, we present high-quality, haplotype-resolved, chromosome-level genome assembly for deyangensis (hereinafter referred to ‘Deyangshi’), an autotetraploid notable its short juvenile phase, by integrating high-fidelity single-molecule, nanopore sequencing, high-throughput chromosome conformation capture techniques....
Grafting, an ancient horticultural cultivation technique, is essential for commercial apple (Malus × domestica Borkh.) production. M. prunifolia 'Fupingqiuzi' used as a common rootstock due to its known tolerances abiotic and biotic stresses. However, the genetic origin evolutionary history of are largely unknown, molecular bases underlying innate stress tolerance characteristics ability graft-transmit scion. Here, we present high-quality chromosome-scale genome sequence (2n = 2x 34)...
Abstract Persimmon anthracnose, a severe disease caused by the hemibiotrophic fungus Colletotrichum horii , poses substantial threat to China’s persimmon industry. Previous research showed that ‘Kangbing Jianshi’ cultivar exhibits strong resistance anthracnose. Notably, branches exhibit greater lignification compared with susceptible ‘Fuping cultivar. In this study, higher lignin content was observed in Jianshi’, and difference associated resistance. Transcriptome metabolome analyses...
Water deficit is one of the main limiting factors for apple growth and production. Root architecture plays an important role in drought tolerance plants. However, little known about molecular basis root system trees under drought. In this study, we compared morphology two widely used rootstocks (R3 Malus sieversii) Our results suggested that M. sieversii more tolerant to than R3, since had a higher ratio shoot as well hydraulic conductivity long-term conditions. We then performed...
Drought limits apple yield and fruit quality. However, the molecular mechanism of in response to drought is not well known. Here, we report a Cys2/His2 (C2H2)-type zinc-finger protein, MdZAT5, that positively regulates tolerance by regulating drought-responsive RNAs microRNAs (miRNAs). DNA affinity purification sequencing yeast-one hybrid analysis identified binding motifs T/ACACT/AC/A/G. Chromatin immunoprecipitation quantitative polymerase chain reaction (ChIP-qPCR) electrophoretic...
Medicinal plants, as medicinal materials and important drug components, have been used in traditional folk medicine for ages. However, being sessile organisms, they are seriously affected by extreme environmental conditions abiotic stresses such salt, heavy metal, temperature, water stresses. plants usually produce specific secondary metabolites to survive stresses, these can often be treating human diseases. Recently, found partner with endophytic fungi form a long-term, stable, win-win...
Persimmons (Diospyros kaki Thunb.) have a longstanding history of cultivation in China. Both aesthetically pleasing and edible, they often symbolize sweet fulfilling life. During the summer 2022, severe outbreak anthracnose was observed on lower leaves persimmon trees National Field Genebank for Persimmon (NFGP), located Yangling, Shaanxi, China (34°17′42.80″ N, 108°04′08.21″ E). The estimated incidence rate this disease within NFGP approximately 30%. typical symptoms included presence...
Jasmonate ZIM-domain (JAZ) acts as the repressor of JA signaling pathway and plays a significant role in stress-inducible defense, hormone crosstalk, regulation growth-defense tradeoff. The aim this study is to systematically survey analyze JAZ gene family
As a precursor of aromatic compounds, fatty acids play important roles in apple fruit quality; however, the genetic and molecular basis underlying acid synthesis metabolism is largely unknown. In this study, we conducted genome-wide association study (GWAS) seven using genomic data 149 Malus accessions identified 232 significant signals (-log10P>5) associated with 99 genes from GWAS four across 2 years. Among these, signal linoleic was transcriptional regulator SUPERMAN-like (SUP)...
A series of N ‐nitrourea derivatives bearing various aryl substituents were conveniently obtained via three steps including nitration, carbamic chlorination, and aminolysis reactions. The structures all newly synthesized compounds characterized confirmed by IR, 1 H‐NMR, MS, elemental analysis. preliminary bioassays indicate that five possess sufficient fungicidal activity against Rhizoctonia solani . Structure–activity relationship (SAR) is also discussed based on the experimental data,...