A5101572754- Plant Molecular Biology Research
- Photosynthetic Processes and Mechanisms
- Light effects on plants
- Wheat and Barley Genetics and Pathology
- Genetic Mapping and Diversity in Plants and Animals
- Plant Reproductive Biology
- Plant nutrient uptake and metabolism
- Plant Gene Expression Analysis
- Plant Stress Responses and Tolerance
- Crop Yield and Soil Fertility
- Plant Disease Resistance and Genetics
- Legume Nitrogen Fixing Symbiosis
- Meteorological Phenomena and Simulations
- Immune cells in cancer
- RNA Research and Splicing
- Plant Pathogens and Resistance
- Cancer Immunotherapy and Biomarkers
- Plant-Microbe Interactions and Immunity
- Plant tissue culture and regeneration
- Plant Genetic and Mutation Studies
- Plant Physiology and Cultivation Studies
- ATP Synthase and ATPases Research
- Plant Micronutrient Interactions and Effects
- Greenhouse Technology and Climate Control
- Insurance and Financial Risk Management
Peking University
2013-2025
Shandong Academy of Agricultural Sciences
2023-2025
National Clinical Research Center for Digestive Diseases
2025
Nanjing Normal University
2024-2025
Southern University of Science and Technology
2021-2024
Institute of Plant Biology
2024
Planta
2024
Peking University Cancer Hospital
2024
Research Institute of Forestry
2024
Forestry Research Institute
2024
Plants have evolved a delicate molecular system to fine-tune their growth and development in response dynamically changing light environments. In this study, we found that BBX28, B-box domain protein, negatively regulates photomorphogenic dose-dependent manner Arabidopsis thaliana. BBX28 interferes with the binding of transcription factor HY5 promoters its target genes through physical interactions, thereby repressing activity affecting HY5-regulated gene expression. darkness, associates...
Significance Initially discovered as a central repressor of the seedling photomorphogenesis in plants, and later characterized animals, E3 ligase CONSTITUTIVELY PHOTOMORPHOGENIC 1 (COP1) has been shown to have conserved biochemical activities eukaryotes. In this study, we show that coordinately promote skotomorphogenesis, COP1/ SUPPRESSOR phyA-105 (SPA) complex represses activity GSK3-like kinase BRASSINOSTEROID-INSENSITIVE 2 (BIN2) nonproteolytic manner stabilize key PHYTOCHROME INTERACTING...
B-box-containing (BBX) proteins play critical roles in a variety of cellular and developmental processes plants. BBX21 (also known as SALT TOLERANCE HOMOLOG2), which contains two B-box domains tandem at the N terminus, has been previously demonstrated key component involved COP1-HY5 signaling hub. However, exact molecular physiological are largely unclear. Here, we found that structurally disruption second domain, but not first one, completely abolishes its biological activity conferring...
Abstract ELONGATED HYPOCOTYL 5 (HY5), a basic domain/leucine zipper (bZIP) transcription factor, acts as master regulator of to promote photomorphogenesis. At present, it’s unclear whether HY5 uses additional mechanisms inhibit hypocotyl elongation. Here, we demonstrate that enhances the activity GSK3-like kinase BRASSINOSTEROID-INSENSITIVE 2 (BIN2), key repressor brassinosteroid signaling, repress We show physically interacts with and genetically through BIN2 The interaction its possibly by...
Leaf rust, caused by Puccinia triticina Eriksson (Pt), is one of the most severe foliar diseases wheat. Breeding for leaf rust resistance a practical and sustainable method to control this devastating disease. Here, we report identification Lr47, broadly effective gene introgressed into wheat from Aegilops speltoides. Lr47 encodes coiled-coil nucleotide-binding leucine-rich repeat protein that both necessary sufficient confer Pt resistance, as demonstrated loss-of-function mutations...
The CONSTITUTIVE PHOTOMORPHOGENIC 1-SUPPRESSOR OF PHYA-105 (COP1-SPA) complex is a central repressor of photomorphogenesis. This acts as an E3 ubiquitin ligase downstream various light signaling transduced from multiple photoreceptors in plants. How the COP1-SPA activity regulated by divergent light-signaling pathways remains largely elusive. Here, we reproduced regulation pathway ultraviolet-B (UV-B) vitro and determined cryo-electron microscopy structure UV-B receptor UVR8 with COP1....
Abstract Ambient light and the endogenous circadian clock play key roles in regulating Arabidopsis (Arabidopsis thaliana) seedling photomorphogenesis. PHYTOCHROME-INTERACTING FACTOR 4 (PIF4) acts downstream of both to promote hypocotyl elongation. Several members R2R3-MYB transcription factor (TF) family, most common type MYB TF family Arabidopsis, have been shown be involved Nonetheless, whether TFs are connecting signaling pathways during photomorphogenesis remains unknown. Here, we report...
Abstract Intron retention (IR) is the most common alternative splicing event in Arabidopsis . An increasing number of studies have demonstrated major role IR gene expression regulation. The impacts on plant growth and development response to environments remain underexplored. Here, we found that functions directly regulation a genome-wide scale through detainment intron-retained transcripts (IRTs) nucleus. Nuclear-retained IRTs can be kept away from translation this mechanism. COP1-dependent...
Abstract Light serves as the energy source for plants well a signal growth and development during their whole life cycle. Seedling de-etiolation is most dramatic manifestation of light-regulated plant processes, massive reprogramming transcriptome occurs at this time. Although several studies have reported about organ-specific expression induced by light, systematic analysis cell-type-specific differentiation associated transcriptional regulation still lacking. Here we obtained single-cell...
A functional female gametophyte is the basis of successful sexual reproduction in flowering plants. During development, megaspore mother cell (MMC), which differentiates from a single subepidermal somatic nucellus, undergoes meiosis to produce four megaspores; only one at chalazal end, referred as (FM), then three rounds mitosis and develops into mature embryo sac. Here, we report that RING1A RING1B (RING1A/B), two functionally redundant Polycomb proteins Arabidopsis, are critical for...
Abstract In plants, microRNAs (miRNAs) participate in complex gene regulatory networks together with the transcription factors (TFs) response to biotic and abiotic stresses. To date, analyses of miRNAs‐induced transcriptome remodeling are at whole plant or tissue levels. Here, Arabidopsis ’s ABA‐induced single‐cell RNA‐seq (scRNA‐seq) is performed different stages time points–early, middle, late. Single‐cell level primary miRNAs (pri‐miRNAs) atlas supported rapid, dynamic, cell‐type specific...
Abstract CONSTITUTIVELY PHOTOMORPHOGENIC 1 (COP1), which was first discovered as a central repressor of photomorphogenesis in Arabidopsis , destabilizes proteins by ubiquitination both plants and animals. However, it is unclear whether how COP1 mediates non-proteolytic to regulate photomorphogenesis. Here, we show that COP1-mediated lysine 63 (K63)-linked polyubiquitination inhibits the enzyme activity GRETCHEN HAGEN 3.5 (GH3.5), synthetase conjugates amino acids indole-3-acetic acid (IAA),...
Polycomb group (PcG) protein-mediated gene silencing is a major regulatory mechanism in higher eukaryotes that affects expression at the transcriptional level. Here, we report two conserved homologous PcG proteins, RING1A and RING1B (RING1A/B), are required for global H2A monoubiquitination (H2Aub) Arabidopsis. The mutation of RING1A/B increased members SQUAMOSA PROMOTER BINDING PROTEIN-LIKE (SPL) family caused an early vegetative phase transition. transition observed ring1a ring1b double...
Abstract Background Gastric cancer with peritoneal metastasis (PM-GC), recognized as one of the deadliest cancers. However, whether and how tumor cell-extrinsic microenvironment (TME) is involved in therapeutic failure remains unknown. Thus, this study systematically assessed immunosuppressive ascites from patients PM-GC, its contribution to dissemination immune evasion ascites-disseminated cells (aDTCs). Methods Sixty-three 43 peripheral blood (PB) samples 51 PM-GC were included study....
Light-triggered transcriptome reprogramming is critical for promoting photomorphogenesis in Arabidopsis seedlings. Nonetheless, recent studies have shed light on the importance of alternative pre-mRNA splicing (AS) photomorphogenesis. The factors factor phytochrome signaling (SFPS) and reduced red-light responses cry1cry2 background1 (RRC1) are involved B (phyB) pathway promote by controlling light- clock-related genes. However, that serve as repressors phyB remain unreported. Here, we...
MicroRNAs (miRNAs) are trans-acting small regulatory RNAs that work coordinately with transcription factors (TFs) to shape the repertoire of cellular mRNAs available for translation. Despite our growing knowledge individual plant miRNAs, their global roles in gene networks remain mostly unassessed. Based on interactions obtained from public databases and curated literature, we reconstructed an integrated miRNA network Arabidopsis includes 66 core TFs, 318 1712 downstream genes. We found...
CONSTITUTIVELY PHOTOMORPHOGENIC 1 (COP1) mediates various cellular and physiological processes in plants by targeting a large number of substrates for ubiquitination degradation. In this study, we reveal that substitution Pro Leu at amino acid position 409 WRKY32 largely suppresses the short hypocotyls expanded cotyledon phenotypes cop1-6. WRKY32P409L promotes hypocotyl growth inhibits opening cotyledons Arabidopsis. Loss function mutant seedlings display elongated hypocotyls, whereas...
Leaf, spike, stem, and root morphologies are key factors that determine crop growth, development, productivity. Multiple genes control these morphological traits have been identified in Arabidopsis, rice, maize, other plant species. However, little is known about the genomic regions associated with wheat. Here, we ethyl methanesulfonate-derived mutant wheat line M133 displays multiple changes include upward-curled leaves, paired spikelets, dwarfism, delayed heading. Using bulked segregant...
With intrinsic acetyltransferase activities, CREB binding protein (CBP)/p300 proteins mediate a variety of physiological events, such as proliferation, differentiation, and apoptosis, by regulating both histones non-histone proteins. Arabidopsis CBP-type histone family proteins, HACs, have been found to influence flower the expression Flowering Locus C. We recently reported that HAC genes involved in ethylene signaling pathway. Thereafter, we systematically analyzed morphological...