A5020350379- Plant Gene Expression Analysis
- Plant biochemistry and biosynthesis
- Photosynthetic Processes and Mechanisms
- Plant Molecular Biology Research
- Plant nutrient uptake and metabolism
- Plant tissue culture and regeneration
- Soybean genetics and cultivation
- Biochemical Analysis and Sensing Techniques
- Enzyme Production and Characterization
- Biochemical and biochemical processes
- Polysaccharides and Plant Cell Walls
- Phytochemicals and Antioxidant Activities
- Plant Parasitism and Resistance
- Plant pathogens and resistance mechanisms
- Plant-derived Lignans Synthesis and Bioactivity
- Phytochemicals and Medicinal Plants
- Genomics, phytochemicals, and oxidative stress
RIKEN Center for Sustainable Resource Science
2011-2025
Japan Women's University
2010
Tokyo Metropolitan University
2009
William V. S. Tubman University
1996
The phytohormone auxin plays critical roles in the regulation of plant growth and development. Indole-3-acetic acid (IAA) has been recognized as major for more than 70 y. Although several pathways have proposed, how is synthesized plants still unclear. Previous genetic enzymatic studies demonstrated that both TRYPTOPHAN AMINOTRANSFERASE OF ARABIDOPSIS (TAA) YUCCA (YUC) flavin monooxygenase-like proteins are required biosynthesis IAA during development, but these enzymes were placed two...
Auxins are hormones that regulate many aspects of plant growth and development. The main auxin is indole-3-acetic acid (IAA), whose biosynthetic pathway not fully understood. Indole-3-acetaldoxime (IAOx) has been proposed to be a key intermediate in the synthesis IAA several other indolic compounds. Genetic studies biosynthesis Arabidopsis have suggested 2 distinct pathways involving CYP79B or YUCCA ( YUC ) genes may contribute IAOx also involved conversion IAA. Here we report biochemical...
To identify candidate genes involved in Arabidopsis flavonoid biosynthesis, we applied transcriptome coexpression analysis and independent component analyses with 1388 microarray data from publicly available databases. Two glycosyltransferases, UGT79B1 UGT84A2 were found to cluster anthocyanin biosynthetic genes. Anthocyanin was drastically reduced ugt79b1 knockout mutants. Recombinant protein converted cyanidin 3-O-glucoside 3-O-xylosyl(1→2)glucoside. recognized 3-O-glucosylated...
The phytohormone auxin plays a central role in many aspects of plant growth and development. IAA is the most studied natural that possesses property polar transport plants. Phenylacetic acid (PAA) has also been recognized as for >40 years, but its development remains unclear. In this study, we show PAA have overlapping regulatory roles distinct characteristics auxins widely distributed vascular non-vascular Although biological activities are lower than those IAA, endogenous levels much...
Abstract Land plants produce diverse flavonoids for growth, survival, and reproduction. Chalcone synthase is the first committed enzyme of flavonoid biosynthetic pathway catalyzes production 2′,4,4′,6′-tetrahydroxychalcone (THC). However, it also produces other polyketides, including p -coumaroyltriacetic acid lactone (CTAL), because derailment chalcone-producing pathway. This promiscuity CHS catalysis adversely affects efficiency biosynthesis, although believed to have led evolution...
Summary Flavonol 3‐ O ‐diglucosides with a 1→2 inter‐glycosidic linkage are representative pollen‐specific flavonols that widely distributed in plants, but their biosynthetic genes and physiological roles not well understood. Flavonoid analysis of four Arabidopsis floral organs (pistils, stamens, petals calyxes) flowers wild‐type male sterility 1 ( ms1 ) mutants, which defective normal development pollen tapetum, showed kaempferol/quercetin O‐ β‐ d ‐glucopyranosyl‐(1→2)‐β‐ ‐glucopyranosides...
A large part of chemodiversity plant triterpenes is due to the modification their side chains. Reduction or isomerization double bonds in chains often an important step for diversification triterpenes, although enzymes involved are not fully understood. Withanolides a group structurally diverse C28 steroidal lactones derived from 24-methylenecholesterol. These compounds found Indian medicinal Withania somnifera, also known as ashwagandha, and other members Solanaceae. The pathway withanolide...
Abstract Lignans/neolignans are generally synthesized from coniferyl alcohol (CA) in the cinnamate/monolignol pathway by oxidation to generate corresponding radicals with subsequent stereoselective dimerization aided dirigent proteins (DIRs). Genes encoding oxidases and DIRs for neolignan biosynthesis have not been identified previously. In Arabidopsis thaliana, DIR AtDP1/AtDIR12 plays an essential role 8-O-4′ coupling unequivocal structural determination of compound missing atdp1 mutant as...
Steviol glycosides (SGs) are noncaloric natural sweeteners found in the leaves of stevia (Stevia rebaudiana). These diterpene biosynthesized by attaching varying numbers monosaccharides, primarily glucose, to steviol aglycone. Rebaudioside (Reb) D and Reb M highly glucosylated SGs that valued for their superior sweetness organoleptic properties, yet they present limited quantities leaves. This study aims improve substrate preference catalytic efficiency UDP-sugar-dependent...
UGT79B31 encodes flavonol 3- O -glycoside: 2″- -glucosyltransferase, an enzyme responsible for the terminal modification of pollen-specific flavonols in Petunia hybrida. Flavonoids are known to be involved pollen fertility petunia (P. hybrida) and maize (Zea mays). As a first step toward elucidating role flavonoids pollen, we have identified glycosyltransferase that is flavonoids. An silico search transcriptome database revealed four candidate UDP-glycosyltransferase (UGT) genes. was...
Flavonol glycosides (FGs) are major components of soybean leaves and there substantial differences in FG composition among genotypes. The first objective this study was to identify genes responsible for biosynthesis locate them the genome. second clone candidate verify their function. Recombinant inbred lines (RILs) were developed from a cross between cultivars Nezumisaya Harosoy.HPLC comparison with authentic samples suggested that FGs having glucose at 2″-position or galactose is bound...
Abstract Plants produce specialized metabolites with defensive properties that are often synthesized through the coordinated regulation of metabolic genes by transcription factors in various biological contexts. In this study, we investigated regulatory function factor PhERF1 from petunia (Petunia hybrida), which belongs to a small group ETHYLENE RESPONSE FACTOR (ERF) family members regulate biosynthesis bioactive alkaloids and terpenoids plant lineages. We examined effects transiently...