A5078869221- Potato Plant Research
- Photosynthetic Processes and Mechanisms
- Silk-based biomaterials and applications
- RNA Interference and Gene Delivery
- Transgenic Plants and Applications
- Plant Molecular Biology Research
- Plant nutrient uptake and metabolism
- Plant Disease Resistance and Genetics
- Biochemical and Structural Characterization
- Plant Reproductive Biology
- Studies on Chitinases and Chitosanases
- Light effects on plants
- Phytochemical compounds biological activities
- Invertebrate Immune Response Mechanisms
- Genomics and Phylogenetic Studies
- Photoreceptor and optogenetics research
- Protist diversity and phylogeny
- Algal biology and biofuel production
- Protein Hydrolysis and Bioactive Peptides
- Insect symbiosis and bacterial influences
- Legume Nitrogen Fixing Symbiosis
- Berry genetics and cultivation research
- Bacteriophages and microbial interactions
- Cassava research and cyanide
- Mitochondrial Function and Pathology
Kyoto University
2022-2025
Utsunomiya University
2023-2024
Kyoto Katsura Hospital
2023-2024
RIKEN Center for Sustainable Resource Science
2019-2021
Nara Institute of Science and Technology
2016-2019
Mahidol University
2012
National Center for Genetic Engineering and Biotechnology
2012
Steroidal glycoalkaloids (SGAs) are cholesterol-derived specialized metabolites produced in species of the Solanaceae. Here, we report that a group jasmonate-responsive transcription factors ETHYLENE RESPONSE FACTOR (ERF) family (JREs) close homologs alkaloid regulators Cathranthus roseus and tobacco, regulate production SGAs tomato. In transgenic tomato, overexpression dominant suppression JRE genes caused drastic changes SGA accumulation expression for metabolic enzymes involved multistep...
Summary Steroidal glycoalkaloids ( SGA s) are specialized anti‐nutritional metabolites that accumulate in Solanum lycopersicum (tomato) and tuberosum (potato). A series of biosynthetic genes is known to be upregulated Solanaceae species by jasmonate‐responsive Ethylene Response Factor transcription factors, including JRE 4 (otherwise as GAME 9), but the exact regulatory significance planta each factor has remained unaddressed. Here, via TILLING ‐based screening an EMS ‐mutagenized tomato...
Genetic engineering of economically important traits in plants is an effective way to improve global welfare. However, introducing foreign DNA molecules into plant genomes create genetically engineered not only requires a lengthy testing period and high developmental costs but also well-accepted by the public due safety concerns about its effects on human animal health environment. Here, we present high-throughput nucleic acids delivery platform for using peptide nanocarriers applied leaf...
Abstract The plastid is an organelle that functions as a cell factory to supply food and oxygen the plant therefore potential target for genetic engineering acquire plants with novel photosynthetic traits or ability produce valuable biomolecules. Conventional genome technologies are laborious preparation of material, require expensive experimental instruments, time consuming obtaining transplastomic line produces significant levels biomolecule interest. Herein, transient transformation...
Abstract Photosynthetic microorganisms such as cyanobacteria, purple bacteria and microalgae have attracted great interest promising platforms for economical sustainable production of bioenergy, biochemicals, biopolymers. Here, we demonstrate heterotrophic spider dragline silk proteins, major ampullate spidroins (MaSp), in a marine photosynthetic bacterium, Rhodovulum sulfidophilum , under both photoheterotrophic photoautotrophic growth conditions. Spider is biodegradable biocompatible...
SUMMARY Spider silk, especially dragline silk from golden spiders ( Trichonephila clavipes ), is an excellent natural material with remarkable mechanical properties. Many studies have focused on the use of plants as biofactories for production recombinant spider silk. However, effects this properties or physiology transgenic remain poorly understood. Since glycine‐rich proteins play key roles in plants, we evaluated a protein plant (mechanotype) and physiology. We generated tobacco Nicotiana...
Simple and efficient cell-penetrating peptide-based multiple DNA, RNA protein codelivery platforms to plant cells have been developed.
Plant mitochondria move dynamically inside cells and this movement is classified into two types: directional movement, in which travel long distances, wiggling, short distances. However, the underlying mechanisms roles of both types mitochondrial especially remain to be determined. Here, we used confocal laser-scanning microscopy quantitatively characterize (rate trajectory) Arabidopsis thaliana mesophyll cells. Directional leading long-distance migration occurred at high speed with a low...
Abstract The remarkable mechanical strength and extensibility of spider dragline silk spidroins are attributed to the major ampullate proteins (MaSp). Although fragmented MaSp molecules have been extensively produced in various heterologous expression platforms for biotechnological applications, complete required achieve instinctive spinning spidroin fibers from aqueous solutions. Here, a plant cell‐based platform extracellular production entire MaSp2 protein is developed, which exhibits...
Abstract An important method for plant genetic modification is using peptide/pDNA complexes to transfer genes into cells. With conventional carrier peptides, the peptide sequence must contain a high amount of cationic amino acids condense and introduce pDNA. As result, dissociation pDNA from complex inefficient, often causing problems. Herein, we designed new that mimics basic leucine zipper (bZIP) domain DNA-binding proteins, in which (LU) 4 motif (KUA) 3 cell-penetrating (U =...
Chloroplast photorelocation is a vital organellar response that optimizes photosynthesis in plants amid fluctuating environmental conditions. Chloroplasts exhibit an accumulation response, which they move toward weak light to enhance photoreception, and avoidance away from strong avoid photodamage. Although chloroplast has been extensively studied model such as
Abstract Chloroplasts accumulate in regions of plant cells exposed to irradiation maximize light reception for efficient photosynthesis. This response is mediated by the blue‐light receptor phototropin. Upon perception blue light, phototropin photoactivated, an unknown signal transmitted from photoactivated distant chloroplasts, and chloroplasts begin their directional movement. How activated initiates this transmission unknown. Here, using liverwort Marchantia polymorpha , we analysed...
In Solanum lycoperisicum (tomato), a transcription factor of APETALA2/ETHYLENE RESPONSE FACTOR (AP2/ERF) family, JASMONATE-RESPONSIVE ERF 3 (JRE3), is closest homolog JRE4, master transcriptional regulator steroidal glycoalkaloid (SGA) biosynthesis. tomato genome, JRE3 resides in gene cluster with JRE4 and related JRE1, JRE2, JRE5, while JRE6 exists as singleton on different chromosome. All the JREs are induced by jasmonates (JAs), whereas sodium chloride (NaCl) treatment drastically...
In article 1902064, Keiji Numata and co-workers perform targeted gene delivery into various plastids of intact plants using peptide/plasmid DNA complexes that combine the functions both a cell-penetrating peptide chloroplast-targeting peptide. This technology will provide useful tool to rapidly engineer in crop species.
Plastids are pivotal target organelles for comprehensively enhancing photosynthetic and metabolic traits in plants via plastid engineering. Plastidial proteins predominantly originate the nucleus must traverse membrane-bound multiprotein translocons to access these organelles. This import process is meticulously regulated by chloroplast-targeting peptides (cTPs). Whereas many cTPs have been employed guide recombinantly expressed functional chloroplasts, there a critical need more efficient...
Recombinant Spider Silk Proteins In article number 2300011, Keiji Numata and co-workers create a plant-based system to produce secretory spider silk using tobacco cell suspension. Despite plant cells excelling in the high production of recombinant proteins, secretion spidroins is intricately controlled by extracellular protein sorting mechanisms, specifically related C-terminal domain.