A5039777895- Protein Degradation and Inhibitors
- Ubiquitin and proteasome pathways
- Plant tissue culture and regeneration
- Plant Disease Management Techniques
- Viral Infectious Diseases and Gene Expression in Insects
- Chemical Reaction Mechanisms
- Adipose Tissue and Metabolism
- Fusion materials and technologies
- Axial and Atropisomeric Chirality Synthesis
- CAR-T cell therapy research
- Neuropeptides and Animal Physiology
- ATP Synthase and ATPases Research
- CRISPR and Genetic Engineering
- Peroxisome Proliferator-Activated Receptors
- Synthesis and Properties of Aromatic Compounds
- Electromagnetic Effects on Materials
- Non-Destructive Testing Techniques
- Glycosylation and Glycoproteins Research
Meiji University
2024-2025
University of Tsukuba
1998-2024
Grafting is an agricultural technique that joins tissues from different plants to obtain useful rootstock traits. However, cellular processes involved in joint tissue repair remain poorly understood. We analyzed Nicotiana benthamiana (Nb) and Arabidopsis thaliana (At) interfamily heterografting as a high-stress model At homografting low-stress model. Transmission electron micrographs reveal the formation of autophagic structures cells near graft boundary over long period Nb/At grafts short...
The chemogenetic control of cellular protein stability using degron tags is a powerful experimental strategy in biomedical research. However, this technique requires permanent fusion the to target protein, which may interfere with proper function protein. Here, we report peptide fragment from carboxyl terminus ubiquitin as cleavable linker that exhibits slow but efficient cleavage tag via deubiquitinating enzymes (DUBs). We designed consisting and destabilizing domain (DD), conditionally...
Covalent agonists of PPARγ cause unique receptor conformational changes and behave as selective modulators, whereas there are few covalent other than endogenous unsaturated fatty acids metabolites. Previously, we established a cell-based strategy to identify new ligands synthesized new-type agonist that possesses the hybrid structure plant-derived cinnamic acid derivative GW9662, antagonist. Herein, report six analogues differ in how two fragments linked together. Compounds with simplified...
Proteins are fundamental biomolecules of living cells, and their expression levels depend on the balance between synthesis degradation. Researchers often aim to control protein for investigation function its relationship with physiological phenomena. The genetic manipulation target using CRISPR/Cas9, Cre/loxP, tetracyclin system, RNA interference, widely used regulation proteins at DNA, transcriptional, or mRNA level. However, significant time delay in controlling is a limitation these...
Abstract Aza[6]helicene and aza[7]helicene were synthesized by Fridel‐Crafts‐type cyclization of axially chiral compounds. From the compounds prepared Fischer indole synthesis, aza[6]helicene synthesized. Aza[7]helicene was also from an pyrrole compound Piloty‐Robinson‐type synthesis through simultaneous on both sides ring. isomerized readily at room temperature, while isomerization temperature less likely to proceed. The cell growth inhibitory activity each enantiomer N ‐Me‐aza[7]helicene...
We have successfully applied a bump-and-hole approach to establish orthogonal deubiquitination in which ubiquitin substrate variant is specifically targeted by an engineered deubiquitinating enzyme (DUB). This makes it possibe selectively observe and measure single type of DUB activity living cells.