A5051746008- Plant-Microbe Interactions and Immunity
- Pharmaceutical and Antibiotic Environmental Impacts
- Microbial Community Ecology and Physiology
- Legume Nitrogen Fixing Symbiosis
- Antibiotic Resistance in Bacteria
- Gut microbiota and health
- Genomics and Phylogenetic Studies
- Wastewater Treatment and Nitrogen Removal
- Nematode management and characterization studies
- Soil Carbon and Nitrogen Dynamics
- Plant nutrient uptake and metabolism
- Plant Pathogens and Fungal Diseases
- Microbial Natural Products and Biosynthesis
- Mycorrhizal Fungi and Plant Interactions
- Wastewater Treatment and Reuse
- Aquaculture disease management and microbiota
- Antibiotic Use and Resistance
- Plant responses to water stress
- Polysaccharides and Plant Cell Walls
- Antibiotics Pharmacokinetics and Efficacy
- Animal Nutrition and Physiology
- SARS-CoV-2 detection and testing
- Marine and coastal ecosystems
- Water Treatment and Disinfection
- Biosensors and Analytical Detection
Agricultural Research Organization
2016-2025
University of Minnesota
2007-2009
Institute for Soil, Climate and Water
2007-2009
Twin Cities Orthopedics
2009
Biotechnology Institute
2007-2008
University of Minnesota, Duluth
2007
Institut de Recherche pour le Développement
2007
Laboratoire des Symbioses Tropicales et Méditerranéennes
2007
Genoscope
2007
Centre National de la Recherche Scientifique
2007
Leguminous plants (such as peas and soybeans) rhizobial soil bacteria are symbiotic partners that communicate through molecular signaling pathways, resulting in the formation of nodules on legume roots occasionally stems house nitrogen-fixing bacteria. Nodule has been assumed to be exclusively initiated by binding bacterial, host-specific lipochito-oligosaccharidic Nod factors, encoded nodABC genes, kinase-like receptors plant. Here we show complete genome sequencing two symbiotic,...
ABSTRACT Adding biochar to soil has environmental and agricultural potential due its long-term carbon sequestration capacity ability improve crop productivity. Recent studies have demonstrated that soil-applied promotes the systemic resistance of plants several prominent foliar pathogens. One mechanism for this phenomenon is root-associated microbial elicitors whose presence somehow augmented in biochar-amended soils. The objective study was assess effect amendment on bacterial community...
Abstract Microbial communities are responsible for biological wastewater treatment, but our knowledge of their diversity and function is still poor. Here, we sequence more than 5 million high-quality, full-length 16S rRNA gene sequences from 740 treatment plants (WWTPs) across the world use to construct ‘MiDAS 4’ database. MiDAS 4 an amplicon variant resolved, reference database with a comprehensive taxonomy domain species level all sequences. We independent dataset (269 WWTPs) show that 4,...
The 'biochar effect' depicts a phenomenon in which biochar soil amendment enhances plant performance by promoting growth and suppressing disease. Although this has been observed numerous studies, the mode of action that explains it is currently unknown. In order to elucidate mechanisms responsible for effect', we comprehensively monitored tomato development resistance foliar fungal pathogen Botrytis cinerea, biochar-amended nonamended soils using native washed biochar, striped labile...
Abstract Biochar, in addition to sequestering carbon, ameliorating soil, and improving plant performance, can impact foliar soilborne diseases. Nevertheless, the mechanisms associated with suppression of diseases improved performances are not well understood. This study is designed establish relationships between biochar-induced changes rhizosphere microbial community structure, taxonomic functional diversity, activity disease enhanced performance a comprehensive fashion. Biochar suppressed...
Surveillance of antibiotic resistance genes (ARGs) has been increasingly conducted in environmental sectors to complement the surveys human and animal under "One-Health" framework. However, there are substantial challenges comparing synthesizing results multiple studies that employ different test methods approaches bioinformatic analysis. In this article, we consider commonly used quantification units (ARG copy per cell, ARG genome, density, 16S rRNA gene, RPKM, coverage, PPM, etc.) for...
Summary. Biochar (charcoal) is the solid co-product of pyrolysis, thermal degradation biomass in absence oxygen. Pyrolysis also yields gaseous and liquid biofuel products. There a growing interest worldwide pyrolysis platform, for at least four reasons: (i) can be source renewable biofuels; (ii) many waste materials treated by thus converted into fuel resource; (iii) long-term sequestration carbon dioxide which originated atmosphere may result from adding biochar to soil, (iv) soil amendment...
ABSTRACT The growth and persistence of rhizobia bradyrhizobia in soils are negatively impacted by drought conditions. In this study, we used genome-wide transcriptional analyses to obtain a comprehensive understanding the response Bradyrhizobium japonicum drought. Desiccation cells resulted differential expression 15 20% 8,480 B. open reading frames, with considerable differentiation between early (after 4 h) late 24 72 expressed genes. While 225 genes were universally up-regulated at all...
Microorganisms carried by dust storms are transported through the atmosphere and may affect human health functionality of microbial communities in various environments. Characterizing dust-borne microbiome different origins or that followed trajectories provides valuable data to improve our understanding global environmental impacts. We present a comparative study on diversity bacterial from three distinct (North Africa, Syria Saudi Arabia) compare them with local sampled clear days, all...
We evaluated the impact of Saharan dust storms on local airborne microbiome in a city Eastern Mediterranean area. Samples particles with diameter less than 10 μm were collected during two spring seasons both dusty and nondusty days. DNA was extracted, partial 16S rRNA gene amplicons sequenced using Illumina platform. Bioinformatic analysis showed effect events diversity atmospheric microbiome. The relative abundance desert soil-associated bacteria increased events, while...
The World Health Organization Global Action Plan recommends integrated surveillance programs as crucial strategies for monitoring antibiotic resistance. Although several national are in place clinical and veterinary settings, no such schemes exist antibiotic-resistant bacteria the environment. In this transnational study, we developed, validated, tested a low-cost easy to implement approach evaluate resistance wastewater treatment plants (WWTPs) by targeting cefotaxime-resistant (CTX-R)...
Scarcity of freshwater for agriculture has led to increased utilization treated wastewater (TWW), establishing it as a significant and reliable source irrigation water. However, years research indicate that if not managed adequately, TWW may deleteriously affect soil functioning plant productivity, pose hazard human environmental health. This review leverages the experience researchers, stakeholders, policymakers from Israel, United-States, Europe present holistic, multidisciplinary...
Flavobacteria are important members of aquatic and terrestrial bacterial communities, displaying extreme variations in lifestyle, geographical distribution genome size. They ubiquitous soil, but often strongly enriched the rhizosphere phyllosphere plants. In this study, we compared a root-associated Flavobacterium that recently isolated, physiologically characterized sequenced, to 14 additional genomes, order pinpoint characteristics associated with its high abundance rhizosphere....
The genus Aeromonas is ubiquitous in aquatic environments encompassing a broad range of fish and human pathogens. strains are known for their enhanced capacity to acquire exchange antibiotic resistance genes therefore, frequently targeted as indicator bacteria monitoring antimicrobial environments. This study evaluated temporal trends diversity two adjacent semi-intensive aquaculture facilities ascertain the effects treatment on resistance. In first facility, sulfadiazine-trimethoprim was...
The presence of antibiotic drug residues, resistant bacteria, and resistance genes in agroecosystems has become a significant area research recent years is growing public health concern. While antibiotics are used both human medicine agricultural practices, the majority their use occurs animal production where historically they have been for growth promotion, addition to prevention treatment disease. widespread application wastes lands play major roles introduction antibiotic-related...
Wastewater (WW) reuse is expected to be increasingly indispensable in future water management mitigate scarcity. However, this increases the risk of antibiotic resistance (AR) dissemination via irrigation. Herein, a conventional (chlorination) and an advanced oxidation process (heterogeneous photocatalysis (HPC)) were used disinfect urban WW same target Escherichia coli <10 CFU/100 mL irrigate lettuce plants (Lactuca sativa) set up four groups, each receiving one types, secondary (positive...
Treated-wastewater (TW) irrigation transfers antibiotic-resistant bacteria (ARB) to soil, but persistence of these is generally low due resilience the soil microbiome. Nonetheless, wastewater-derived and associated antibiotic resistance genes (ARGs) may persist below detection levels potentially proliferate under copiotrophic conditions. To test this hypothesis, we exposed soils from microcosm, lysimeter, field experiments short-term enrichment in copiotroph-stimulating media. In microcosms,...
The vertical and seasonal distribution diversity of archaeal sequences was investigated in a hypersaline, stratified, monomictic lake, Solar Lake, Sinai, Egypt, during the limnological development stratification mixing. Archaeal were studied via phylogenetic analysis 16S rDNA as well denaturing gradient gel electrophoresis analysis. 165 clones grouped into four phylogenetically different clusters. Most isolated from both aerobic epilimnion sulfide-rich hypolimnion defined cluster I,...
Trehalose, a disaccharide accumulated by many microorganisms, acts as protectant during periods of physiological stress, such salinity and desiccation. Previous studies reported that the trehalose biosynthetic genes (otsA, treS, treY) in Bradyrhizobium japonicum were induced desiccation stresses. Functional mutational analyses indicated disruption otsA decreased accumulation cells an treY double mutant extremely low level trehalose. In contrast, to greater extent treS mutant, maltose levels...