A5081349082- Antibiotic Resistance in Bacteria
- Pharmaceutical and Antibiotic Environmental Impacts
- Bacterial biofilms and quorum sensing
- Vibrio bacteria research studies
- Antibiotics Pharmacokinetics and Efficacy
- Enterobacteriaceae and Cronobacter Research
- Salmonella and Campylobacter epidemiology
- Bacterial Identification and Susceptibility Testing
- Antibiotic Use and Resistance
- Infections and bacterial resistance
- Bacteriophages and microbial interactions
- Microbial Community Ecology and Physiology
- Environmental DNA in Biodiversity Studies
- Food Safety and Hygiene
- Bacillus and Francisella bacterial research
- Aquaculture disease management and microbiota
- Plant Pathogenic Bacteria Studies
- Epigenetics and DNA Methylation
- Tuberculosis Research and Epidemiology
- Pneumonia and Respiratory Infections
- Mycobacterium research and diagnosis
- RNA modifications and cancer
- Public Health in Brazil
- SARS-CoV-2 detection and testing
- Escherichia coli research studies
Universidade Federal do Rio de Janeiro
2016-2025
Universidade Federal de São Carlos
2024
Universidade Federal de São Paulo
2007-2018
Blücher (Germany)
2014
Bicêtre Hospital
2008-2009
Assistance Publique – Hôpitaux de Paris
2008-2009
Inserm
2008-2009
Université Paris-Sud
2008
Surveillance of antibiotic resistance genes (ARGs) has been increasingly conducted in environmental sectors to complement the surveys human and animal under "One-Health" framework. However, there are substantial challenges comparing synthesizing results multiple studies that employ different test methods approaches bioinformatic analysis. In this article, we consider commonly used quantification units (ARG copy per cell, ARG genome, density, 16S rRNA gene, RPKM, coverage, PPM, etc.) for...
The aim of this study was to evaluate the presence carbapenemases in a Klebsiella pneumoniae collection and performance modified Hodge test (MHT) correctly identify phenotype.Twenty-eight K. clinical isolates with reduced susceptibility carbapenems were evaluated. Antimicrobial molecular typing performed by agar dilution PFGE, respectively. MHT using both standard high inoculum organisms. Imipenem hydrolysis investigated spectrophotometric assays carbapenemase-encoding genes identified PCR...
We investigated the antimicrobial resistance profile and occurrence of Klebsiella pneumoniae carbapenemase (KPC)-producing Gram-negative rods in sewage samples obtained from a Brazilian teaching hospital wastewater treatment plant (WWTP) that receives it for treatment. identified multidrug-resistant bacteria as well KPC-2-producing Aeromonas spp. several Enterobacteriaceae species, including Kluyvera spp., effluent different sites WWTP. Most isolates showed blaKPC-2 gene harbored on...
This study aimed to identify lactic acid bacteria (LAB) in byproducts of fruit (Malpighia glabra L., Mangifera indica Annona muricata and Fragaria vesca L.) pulp processing. Fifty strains LAB were identified using matrix-assisted laser desorption/ionization-time flight mass spectrometry (MALDI-TOF MS) 16S rRNA gene sequence (16S rRNA) analysis. Species belonging Lactobacillus genus the predominant all processing byproducts. The average congruency between MALDI-TOF MS species identification...
Klebsiella pneumoniae is an important opportunistic pathogen associated with nosocomial and community-acquired infections. A wide repertoire of virulence antimicrobial resistance genes present in K. genomes, which can constitute extra challenges the treatment infections caused by some strains. Kp13 a multidrug-resistant strain responsible for causing large outbreak teaching hospital located Southern Brazil. produces carbapenemase (KPC-2) but unrelated to isolates belonging ST 258 11, main...
ABSTRACT The emergence of metallo-β-lactamase (MBL)-producing isolates is a challenge to routine microbiology laboratories, since there are no standardized methods for detecting such isolates. aim this study was evaluate the accuracy different phenotypic detect MBL production among Pseudomonas spp., Acinetobacter and enterobacterial isolates, including GIM, IMP, SIM, SPM, VIM variants. A total 46 genetically unrelated aeruginosa , putida sp., strains producing distinct MBLs were tested....
A retrospective survey was conducted to characterize beta-lactamases in a collection of 43 ceftazidime-resistant Pseudomonas aeruginosa isolates recovered from patients with bloodstream infections hospitalized at Brazilian teaching hospital between January and December 2005. Resistance rates for carbapenems, aminoglycosides, quinolones were over 80%, only colistin remaining active against all isolates. Pulsed-field gel electrophoresis analysis identified seven different genotypes. AmpC...
Multi-drug efflux pumps have been increasingly recognized as a major component of resistance in P. aeruginosa. We investigated the expression level systems among clinical isolates aeruginosa, regardless their antimicrobial susceptibility profile.Aztreonam exhibited highest vitro activity against aeruginosa studied (64.4% susceptibility), whereas rates imipenem and meropenem were both 47.5%. The MexXY-OprM MexAB-OprM overexpressed 50.8% 27.1% studied, respectively. Overexpression MexEF-OprN...
To search for plasmid-mediated qnr genes among waterborne environmental Aeromonas spp. recovered from Switzerland.Isolates presenting MICs of nalidixic acid or ciprofloxacin > = 1 mg/L were screened by a multiplex PCR approach followed sequencing. Plasmids transferred transformation, and further analysis the genetic structures surrounding qnrS2 gene was carried out sequencing.A identified single allosaccharophila isolate (Lugano lake, Lugano), as part mobile insertion cassette located on...
Carbapenemase-producing bacteria cause difficult-to-treat infections related to increased mortality in health care settings. Their occurrence has been reported raw sewage, sewage-impacted rivers, and polluted coastal waters, which may indicate their spread the community. We assessed variety concentration of carbapenemase producers waters with distinct pollution levels for 1 year. describe various bacterial species producing carbapenemases not only unsuitable but also considered suitable...
The dissemination of plasmid-mediated antimicrobial resistance genes may pose a substantial public health risk. In the present work, occurrences blaCTX-M and ampC qnr were investigated in Escherichia coli from 16 chicken carcasses produced by four commercial brands Brazil. Of tested, three exporters, including one organic chicken. Our study assessed 136 E. isolates that grouped into 77 distinct biotypes defined their origin, profiling, presence β-lactamase quinolone enterobacterial...
To investigate Pseudomonas aeruginosa isolates from a hospital wastewater treatment plant (HWTP), focusing on enzyme‐based mechanisms of β‐lactams resistance and the genetic relatedness among isolates. Forty‐one Ps. strains recovered HWTP were identified by amplification 16S rRNA gene. β‐lactamase production was screened disc diffusion, CHROMagar extended‐spectrum (ESBL) strips. ESBL producing investigated PCR for presence ESBL, metallo‐β‐lactamase Klebsiella pneumoniae carbapenemase...
ABSTRACT Plasmid-mediated qnr genes have been reported in bacteria worldwide and are widely associated with other relevant determinants of resistance multiresistance plasmids. Here, we provide an update on a previously described multiplex PCR order to detect all six families (including qnrA , qnrS qnrB qnrC qnrD qnrVC ) until now. The proposed method makes possible the screening these genes, reducing cost time, it may demonstrate underestimated prevalence latest variants described.
Abstract Background An important virulence factor of Klebsiella pneumoniae is the production capsular polysaccharide (CPS), a thick mucus layer that allows for evasion host's defense and creates barrier against antibacterial peptides. CPS driven mostly by expression genes located in locus called cps , resulting structure used to distinguish between different serotypes (K types). In this study, we report unique genetic organization cluster from K. Kp13, clinical isolate recovered during large...
Gram-negative bacilli producing beta-lactamases are major causes of difficult-to-treat infections, especially the AmpC, ESBL, and carbapenemase types. Their spread within outside hospital settings demands effective detection monitoring in various environments, but current methods for this purpose often neglect important groups or expensive time consuming. We aimed to develop test a massive culture approach detect differentiate between beta-lactamase producers from complex samples. The method...
The Acinetobacter baumannii clonal complex 113/79 (CC113/79) and class 2 integrons predominate in Latin America; a relationship between these characteristics was explored. presence of integrases determined successive hospital isolates (163 A. 72 nosocomialis isolates). Most had integrons, but 1 were present significantly more often CC109/1 CC113/79, respectively. high prevalence CC113/79 America may account for the predominance integrons.
Staphylococcus saprophyticus is an important agent of urinary tract infection (UTI) in young women, but information about this pathogen human microbiota and common environment lacking. The aim study was to characterize S. isolates from genitoanal 621 pregnant 10 minas cheese packs, five beaches Rio de Janeiro city compare PFGE profiles these with UTI clusters described city. We investigated 65 microbiota, 13 cheese, 30 32 isolates. Antimicrobial resistance determined by disk diffusion, MIC...