A5045771904- Antibiotic Resistance in Bacteria
- Pharmaceutical and Antibiotic Environmental Impacts
- Salmonella and Campylobacter epidemiology
- Bacterial biofilms and quorum sensing
- Vibrio bacteria research studies
- Reproductive tract infections research
- Probiotics and Fermented Foods
- Syphilis Diagnosis and Treatment
- Antimicrobial Resistance in Staphylococcus
- Bacterial Infections and Vaccines
- Biochemical and Structural Characterization
- Bacterial Identification and Susceptibility Testing
- Microbial Community Ecology and Physiology
- Enterobacteriaceae and Cronobacter Research
- Genomics and Phylogenetic Studies
- Antimicrobial Peptides and Activities
- Biochemical Analysis and Sensing Techniques
- Enzyme Production and Characterization
- Escherichia coli research studies
- Antibiotic Use and Resistance
- Bacteriophages and microbial interactions
- Food Safety and Hygiene
- Aquaculture disease management and microbiota
- Nanoparticles: synthesis and applications
- Viral gastroenteritis research and epidemiology
Universidade Federal do Rio de Janeiro
2016-2025
University of Bonn
2006
Universidade Federal de Santa Catarina
2000
Summary Lacticin 3147 is a two‐peptide lantibiotic produced by Lactococcus lactis in which both peptides, LtnA1 and LtnA2, interact synergistically to produce antibiotic activities the nanomolar concentration range; individual peptides possess marginal (LtnA1) or no activity (LtnA2). We analysed molecular basis for synergism found cell wall precursor lipid II play crucial role as target molecule. Tryptophan fluorescence measurements identified LtnA1, structurally similar mersacidin, binding...
ABSTRACT The activity of lanthionine-containing peptide antibiotics (lantibiotics) is based on different killing mechanisms which may be combined in one molecule. prototype lantibiotic nisin inhibits peptidoglycan synthesis and forms pores through specific interaction with the cell wall precursor lipid II. Gallidermin epidermin possess same putative II binding motif as nisin; however, both peptides are considerably shorter (22 amino acids, compared to 34 nisin). We demonstrate that model...
A One Health approach for antimicrobial resistance must integrate whole-genome sequencing surveillance data of critical priority pathogens from human, animal and environmental sources to track hot spots routes transmission developing effective prevention control strategies. As part the Grand Challenges Explorations: New Approaches Characterize Global Burden Antimicrobial Resistance Program, we present genomic WHO carbapenemase-resistant, ESBL-producing, and/or colistin-resistant Escherichia...
Carbapenemase-producing bacteria cause difficult-to-treat infections related to increased mortality in health care settings. Their occurrence has been reported raw sewage, sewage-impacted rivers, and polluted coastal waters, which may indicate their spread the community. We assessed variety concentration of carbapenemase producers waters with distinct pollution levels for 1 year. describe various bacterial species producing carbapenemases not only unsuitable but also considered suitable...
ABSTRACT We analyzed the mode of action lantibiotic plantaricin C (PlnC), produced by Lactobacillus plantarum LL441. Compared to well-characterized type A nisin and B mersacidin, which are both able interact with cell wall precursor lipid II, PlnC displays structural features prototypes. In this regard, we found that II plays a key role in antimicrobial activity besides pore formation. The forming whole cells was prevented shielding on surface. However, contrast nisin, not permeabilize...
The dissemination of plasmid-mediated antimicrobial resistance genes may pose a substantial public health risk. In the present work, occurrences blaCTX-M and ampC qnr were investigated in Escherichia coli from 16 chicken carcasses produced by four commercial brands Brazil. Of tested, three exporters, including one organic chicken. Our study assessed 136 E. isolates that grouped into 77 distinct biotypes defined their origin, profiling, presence β-lactamase quinolone enterobacterial...
ABSTRACT Surface waters are considered ecological habitats where Salmonella enterica can persist and disseminate to fresh produce production systems. This study aimed explore the genomic profiles of S. serotypes Typhimurium, Newport, Infantis from surface in Chile, Mexico, Brazil collected between 2019 2022. We analyzed whole genomes 106 S . 161 113 isolates. Our phylogenetic analysis exhibited distinct groupings isolates by their respective countries except for a notable case involving...
ABSTRACT This study examined the diversity and persistence of Salmonella in surface waters agricultural regions Brazil, Chile, Mexico. Research groups (three 2019–2020 five 2021–2022) conducted a long-term survey water across 5–8 months annually ( n = 30 monthly). On-site, each team filtered 10-L samples with modified Moore Swabs to capture Salmonella, which were then isolated identified using conventional microbiological techniques. isolates sequenced on Illumina platforms. was present...
The ability of Staphylococcus aureus to form biofilms is considered an important factor in the pathogenesis central venous catheter-related bacteremia and infections associated with use medical prostheses. Different methods have been described for assessing staphylococcal biofilms, but few comparative studies attempted evaluate these techniques; especially related ica-independent biofilm formation/accumulation. In this study we compared some vitro vivo techniques produced by...
ABSTRACT Plasmid-mediated qnr genes have been reported in bacteria worldwide and are widely associated with other relevant determinants of resistance multiresistance plasmids. Here, we provide an update on a previously described multiplex PCR order to detect all six families (including qnrA , qnrS qnrB qnrC qnrD qnrVC ) until now. The proposed method makes possible the screening these genes, reducing cost time, it may demonstrate underestimated prevalence latest variants described.
Staphylococcus aureus ica-independent biofilms are multifactorial in nature, and various bacterial proteins have been associated with biofilm development, including fibronectin-binding A B, protein A, surface SasG, proteases, some autolysins. The role of extracellular DNA (eDNA) has also demonstrated S. biofilms. Here, we constructed a Tn551 library, the screening identified two genes that affected formation, lrgB yycI. repressive effect both on development was confirmed knockout strains by...
Usually, community-acquired methicillin-resistant Staphylococcus aureus (CA-MRSA) is susceptible to a variety of non-beta-lactam drugs. These isolates commonly display SCCmecIV and are associated with infections. More recently, CA-MRSA has been isolated from health-care-associated diseases. We characterized MRSA 2 hospitals in Rio de Janeiro area assess the entry new lineages. The were primary genotyped using combination molecular typing methods including SCCmec, restriction modification...
The genus Pseudomonas includes metabolically versatile microorganisms occupying diverse niches, from environmental habitats to plant pathogens, and has clinically significant strains. For this reason, spp. might act as a reservoir of antimicrobial resistance genes, which have been detected even in isolated environments. aim study was report the susceptibility profile 25 fluorescens isolates soil samples collected on King George Island (Antarctic Peninsula), select non-clonal with unusual...
Bacterial infections associated with healthcare are a challenge on global scale due to the high morbidity and mortality rates, especially those caused by multidrug-resistant isolates. Hospital textiles abiotic surfaces that may serve as means of disseminating persisting microorganisms in hospitals, can remain viable these for up months. In this study, we employed green synthesis approach utilizing guava leaf extract (Psidium guajava) produce silver nanoparticles, which were then incorporated...
Uropathogenic Escherichia coli (UPEC) is the leading cause of community-acquired urinary tract infection (CA-UTI). The increasing prevalence CA-UTI caused by UPEC strains resistant to broad-spectrum drugs complicates clinical management these infections. Here we assessed antimicrobial drug resistance, genotypes and beta-lactamase genes among isolated from cases in Rio de Janeiro, Brazil during November 2015 determine if drug-resistant determined multiple or dissemination key lineages UPEC....
Resistance in Neisseria gonorrhoeae is a global public health challenge. However, little known about N. isolates from Brazil. In this study, we characterized 116 obtained Rio de Janeiro between 2006 and 2015 according to antimicrobial susceptibility profiles, resistance mechanisms, clonal diversity. We determined minimal inhibitory concentrations by agar dilution, whole genome sequencing was conducted investigate alleles related resistance, determine multilocus sequence typing group based on...