A5069739279- Antibiotic Resistance in Bacteria
- Salmonella and Campylobacter epidemiology
- Escherichia coli research studies
- Enterobacteriaceae and Cronobacter Research
- Bacterial biofilms and quorum sensing
- Vibrio bacteria research studies
- Pharmaceutical and Antibiotic Environmental Impacts
- Bacteriophages and microbial interactions
- Plant Pathogenic Bacteria Studies
- Infections and bacterial resistance
- SARS-CoV-2 detection and testing
- Genomics and Phylogenetic Studies
- Animal Disease Management and Epidemiology
- Virology and Viral Diseases
- Identification and Quantification in Food
- Biosensors and Analytical Detection
- Viral gastroenteritis research and epidemiology
- Zoonotic diseases and public health
- Streptococcal Infections and Treatments
- Mycobacterium research and diagnosis
- Parasitic Infections and Diagnostics
- Fungal Infections and Studies
- Animal Virus Infections Studies
- Antibiotics Pharmacokinetics and Efficacy
Universidade de São Paulo
2021-2025
Hospital Universitário da Universidade de São Paulo
2021
A One Health approach for antimicrobial resistance must integrate whole-genome sequencing surveillance data of critical priority pathogens from human, animal and environmental sources to track hot spots routes transmission developing effective prevention control strategies. As part the Grand Challenges Explorations: New Approaches Characterize Global Burden Antimicrobial Resistance Program, we present genomic WHO carbapenemase-resistant, ESBL-producing, and/or colistin-resistant Escherichia...
Abstract During a microbiological and genomic surveillance study conducted to investigate the molecular epidemiology of extended-spectrum β-lactamase (ESBL)-producing Escherichia coli from community-acquired urinary tract infections (UTI) commercial meat samples, in Brazilian city with high occurrence by ESBL-producing bacteria, we have identified presence CTX-M (-2, -14, -15, -24, -27 -55)-producing E. international clones ST38, ST117, ST131 ST354. The was more prevalent human worryingly...
From a One Health perspective, dogs and cats have begun to be recognized as important reservoirs for clinically significant multidrug-resistant bacterial pathogens. In this study, we investigated the occurrence genomic features of ESβL producing Enterobacterales isolated from dogs, in province Imbabura, Ecuador. We identified four isolates expressing ESβLs healthy diseased animals. regard, two Escherichia coli strains CTX-M-55-like or CTX-M-65 belonged international ST10 ST162, whereas...
Mining dam disasters contribute to the contamination of aquatic environments, impacting associated ecosystems and wildlife. A multidrug-resistant Escherichia coli strain (B2C) was isolated from a river water sample in Brazil after Mariana mining disaster. The genome sequenced using Illumina MiSeq platform, de novo assembled Unicycler. Resistome, virulome, plasmidome were predicted bioinformatics tools. Data analysis revealed that E. B2C belonged sequence type ST219 phylogroup Strikingly,...
Shiga toxin-producing Escherichia coli (STEC) are zoonotic pathogens responsible for causing food-borne diseases in humans. While South America has the highest incidence of human STEC infections, information about genomic characteristics circulating strains is scarce. The aim this study was to analyze data isolated from cattle, beef, and humans; predicting antibiotic resistome, serotypes, sequence types (STs), clonal complexes (CCs) phylogenomic backgrounds. A total 130 whole genome...
The global spread of critical-priority antimicrobial-resistant Enterobacterales by food is a public health problem. Wild-caught seafood are broadly consumed worldwide, but exposure to land-based pollution can favor their contamination clinically relevant bacteria. As part the Grand Challenges Explorations: New Approaches Characterize Global Burden Antimicrobial Resistance Program, we performed genomic surveillance and cell culture-based virulence investigation WHO critical priority isolated...
WHO priority pathogens have disseminated beyond hospital settings and are now being detected in urban wild animals worldwide. In this regard, synanthropic such as pigeons (Columba livia) rodents (Rattus rattus, Rattus norvegicus Mus musculus) of interest to public health due their role reservoirs that can cause severe diseases. These usually live highly contaminated environments frequent interactions with humans, domestic animals, food chain, becoming sentinels anthropogenic activities....
Wild birds have emerged as novel reservoirs and potential spreaders of antibiotic-resistant priority pathogens, being proposed sentinels anthropogenic activities related to the use antimicrobial compounds. The aim this study was investigate occurrence genomic features extended-spectrum β-lactamase (ESBL)-producing bacteria in wild South America. In regard, we identified two ESBL (CTX-M-55 CTX-M-65)-positive Escherichia coli (UNB7 GP188 strains) colonizing Creamy-bellied Thrush (Turdus...
Carbapenemase-producing Enterobacterales are rapidly spreading and adapting to different environments beyond hospital settings. During COVID-19 lockdown, a carbapenem-resistant NDM-1-positive Escherichia coli isolate (BA01 strain) was recovered from pygmy sperm whale (Kogia breviceps), which found stranded on the southern coast of Brazil. BA01 strain belonged global sequence type (ST) 162 carried blaNDM-1, besides other medically important antimicrobial resistance genes. Additionally, genes...
The spread of carbapenemase-producing Klebsiella pneumoniae beyond hospital settings is a global critical issue within public health and One Health perspective. Another worrisome concern the convergence virulence resistance in healthcare-associated lineages K. leading to unfavorable clinical outcomes. During surveillance study WHO priority pathogens circulating an impacted urban river São Paulo, Brazil, we isolate two hypermucoviscous multidrug-resistant strains (PINH-4250 PINH-4900) from...
The aim of this study was to perform a genomic investigation multiple fluoroquinolone-resistant Leclercia adecarboxylata strain isolated from synanthropic pigeon in São Paulo, Brazil.Whole-genome sequencing performed using an Illumina platform, and silico deep analyses the resistome were performed. Comparative phylogenomics conducted global collection publicly available genomes L. strains human animal hosts.L. P62P1 displayed resistance (norfloxacin, ofloxacin, ciprofloxacin, levofloxacin)...
Newcastle disease virus (NDV) can infect over 250 bird species with variable pathogenicity; it also humans in rare cases. The present study investigated an outbreak feral pigeons São Paulo city, Brazil, 2019. Affected birds displayed neurological signs, and hemorrhages were observed different tissues. Histopathology changes infiltration of mononuclear inflammatory cells found the brain, kidney, proventriculus, heart, spleen. NDV staining was detected by immunohistochemistry. Twenty-seven out...
Extended-spectrum β-lactamase (ESBL)–producing Enterobacter cloacae complex (ECC) members have been a leading cause of severe infections in hospital setting and lately recognized as important pathogens for animals. In this article, we report phylogenomic data multidrug-resistant CTX-M-15–positive E. hormaechei belonging to ST78 isolated from calf with omphalitis. Genomic DNA was extracted sequenced using the Illumina NextSeq platform. De novo assembly performed by Unicycler silico prediction...
Convergence of resistance and virulence in Klebsiella pneumoniae is a critical public health issue worldwide. A multidrug-resistant CTX-M-15-producing K. (TIES-4900 strain) was isolated from highly impacted urban river, Brazil. The genome sequenced by MiSeq Illumina platform de novo assembled using Unicycler. In silico prediction accomplished bioinformatics tools. size the 5.4 Mb with 5145 protein-coding genes. TIES-4900 strain belonged to sequence type ST15, yersiniabactin YbST10, ICEKp4,...
The global dissemination of extended-spectrum-β-lactamase (ESBL)-producing Escherichia coli has been considered a critical issue within One Health framework. aim this study was to perform genomic investigation an ESBL-producing E. strain belonging the globally spread sequence type/clonal complex ST90/CC23, isolated from gastrointestinal tract dog, in Brazil. Besides CTX-M-15 ESBL, isolate carried mutations conferring resistance human and veterinary fluoroquinolones (GyrA [Ser83Leu,...
Emergent hypervirulent Klebsiella pneumoniae has been responsible for severe diseases, representing a serious threat to public health. We report the whole-genome sequencing of novel ST3994-K2 clone, single locus variant ST86 K2, which is considered worrying clone that emerged in several parts world. The strain K. pneumonia Kpi144 was isolated 2013 from blood culture 69-year-old male patient admitted tertiary hospital Teresina, state Piauí, northeastern Brazil. susceptible 41 antibiotics...
Journal Article Healthcare-associated NDM-1-producing Enterobacter hormaechei subsp. xiangfangensis clone ST136 emerging as pathogen of companion animals in Brazil Get access Vanessa C Kobs, Kobs Post-Graduation Program on Health and Environment, Universidade da Região de Joinville (Univille), Joinville, Corresponding author. E-mail: v.kobs@univille.br https://orcid.org/0000-0001-6346-2278 Search for other works by this author on: Oxford Academic PubMed Google Scholar Francielle Medeiros,...
The global spread of extended-spectrum β-lactamase (ESβL)-producing Escherichia coli has been considered a One Health issue that demands continuous genomic epidemiology surveillance in humans and non-human hosts. To report the occurrence data ESβL-producing E. strains isolated from South American llamas inhabiting protected area with public access Andean Highlands Peru. Two (E. L1LB L2BHI) were identified by MALDI-TOF. Genomic DNAs extracted sequenced using Illumina NextSeq platform. De novo...
<title>Abstract</title> Contamination of food by critical priority third-generation cephalosporin-resistant Escherichia coli poses a significant risk to consumers, potentially leading foodborne illnesses, silent colonization, and/or antibiotic resistance dissemination. In this study, we report the microbiological and genomic features ESβL/pAmpC-producing E. in conventional (CN) antibiotic-free (AF) retail meat samples sold different markets São Paulo city. Nine representative chicken...