A5059862300- Antibiotic Resistance in Bacteria
- Vibrio bacteria research studies
- Pharmaceutical and Antibiotic Environmental Impacts
- Bacterial biofilms and quorum sensing
- Salmonella and Campylobacter epidemiology
- Parasitic Infections and Diagnostics
- Aquaculture disease management and microbiota
- Enterobacteriaceae and Cronobacter Research
- Antibiotic Use and Resistance
- Escherichia coli research studies
- Amoebic Infections and Treatments
- Environmental DNA in Biodiversity Studies
- Bacterial Genetics and Biotechnology
- Antimicrobial Resistance in Staphylococcus
- SARS-CoV-2 detection and testing
- Bacterial Identification and Susceptibility Testing
- Identification and Quantification in Food
- Antibiotics Pharmacokinetics and Efficacy
- Fecal contamination and water quality
- Genomics and Phylogenetic Studies
- Listeria monocytogenes in Food Safety
- Soil Carbon and Nitrogen Dynamics
- Photodynamic Therapy Research Studies
- Biosensors and Analytical Detection
- Nanoplatforms for cancer theranostics
Universidade de São Paulo
2014-2024
Instituto de Saúde
2010-2023
During a Brazilian multicentric antimicrobial resistance surveillance study, colistin was investigated in 4,620 Enterobacteriaceae isolated from human, animal, food and environmental samples collected 2000 to 2016. We present evidence that mcr-1-positive Escherichia coli has been emerging South America since at least 2012, supporting previous report on the possible acquisition of mcr-1-harbouring E. by European travellers visiting Latin American countries.
A colistin-resistant Escherichia coli strain was recovered from a patient with diabetic foot infection in Brazil. Whole-genome analysis revealed that the E. isolate belonged to widespread sequence type (ST) 101 and harbored mcr-1 gene on an IncX4 plasmid highly similar mcr-1-bearing plasmids were recently identified Enterobacteriaceae food, animal, human samples different continents. These results suggest self-transmissible IncX4-type may represent promiscuous contributing intercontinental...
Antimicrobial blue light (aBL) offers efficacy and safety in treating infections. However, the bacterial targets for aBL are still poorly understood may be dependent on species. Here, we investigated biological of killing by (λ = 410 nm) three pathogens: Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa. Initially, evaluated kinetics bacteria exposed to used this information calculate lethal doses (LD) responsible 90 99.9% bacteria. We also quantified endogenous porphyrins...
Vibrio parahaemolyticus is a marine bacterium, responsible for gastroenteritis in humans. Most of the clinical isolates produce thermostable direct hemolysin (TDH) and TDH-related (TRH) encoded by tdh trh genes respectively. In this study, twenty-three V. parahaemolyticus, previously isolated from oysters mussels were analyzed PCR using specific primers 16S rRNA virulence (tdh, tlh) resistance to different classes antibiotics PFGE. Nineteen confirmed as parahaemolyticus. The tlh gene was...
Abstract Critical priority pathogens have globally disseminated beyond clinical settings, thereby threatening wildlife. Andean Condors ( Vultur gryphus ) are essential for ecosystem health and functioning, but their populations near threatened declining due to anthropogenic activities. During a microbiological genomic surveillance study of critical antibiotic‐resistant pathogens, we identified pandemic lineages multidrug‐resistant extended‐spectrum β‐lactamase (ESBL)‐producing Escherichia...
The unbridled dissemination of multidrug-resistant pathogens is a major threat to global health and urgently demands novel therapeutic alternatives. Antimicrobial photodynamic therapy (aPDT) has been developed as promising approach treat localized infections regardless drug resistance profile or taxonomy. Even though this technique known for more than century, discussions speculations regarding the biochemical mechanisms microbial inactivation have never reached consensus on what primary...
The current COVID-19 pandemic has emphasized the vulnerability of communities living in urban outskirts and informal settlements. lack reliable case data highlights importance application wastewater-based epidemiology. This study aimed to monitor trends four vulnerable (slums low-income neighborhoods) metropolitan São Paulo by assessing SARS-CoV-2 RNA viral load wastewater. We analyzed 160 samples from May 2020 June 2021 with weekly or fortnightly samplings. were ultracentrifuged glycine...
CTX-M enzymes are the most prevalent extended-spectrum beta-lactamases (ESBLs) in Brazil and around world. The spread of lies their ability to be mobilized by insertion sequences integrons. This study aimed identify mobile genetic structures associated with bla(CTX-M) genes from clinical Enterobacteriaceae strains.Twenty-eight non-clonal were screened PCR for presence class 1 integrase (int1), association Plasmid incompatibility groups assessed PBRT. Wild-type plasmids transformed into...
Abstract The presence of Staphylococcus aureus in drinking water is a concern because its potential to cause human infection and also multiple antimicrobial resistance. This study evaluated the quality fountains mist makers four municipal parks São Paulo for 13 months. Although all samples met bacteriological criteria according Brazilian regulations, absence residual chlorine (<0.1 mg/L) was observed. These data were significantly correlated with frequency S. that found 25.2% samples....
This study aims to scrutinize Salmonella spp. and its serotypes in sewage sludge samples from wastewater treatment plants, assesses the presence of virulence genes antibiotics resistant profile. Samples (n = 54) were collected analyzed accordance with EPA Method 1682/2006. For positive serological reaction, 40 strains selected for PCR analyses detection spvC, invA sseL genes, plasmid resistance antibiotics. was detected 38.9% (<0.006473 12.19 MPN/gTS). The most prevalent serotype Infantis....
Abstract The spread of antimicrobial resistance (AMR) through multiple reservoirs is a global concern. Wastewater critical AMR dissemination source, so this study aimed to assess the persistence genetic markers in wastewater using culture‐independent approach. Raw and treated samples ( n = 121) from treatment plant (WWTP), human hospital, veterinary pig farm were monthly collected concentrated by filtration. DNA was extracted directly filter membranes, PCR used qualitative search 32 genes...
To determine the prevalence and expression of metallo-beta-lactamases (MBL)-encoding genes in Aeromonas species recovered from natural water reservoirs southeastern Brazil.Eighty-seven isolates belonging to hydrophila (n = 41) Aer. jandaei 46) were tested for MBL production by combined disk test using imipenem meropenem disks as substrates EDTA or thioglycolic acid inhibitors. The presence was investigated PCR sequencing new consensus primer pairs designed this study. cphA gene found 97.6%...
Extended-spectrum β-lactamases (ESBL) in enterobacteria are recognized worldwide as a great hospital problem. In this study, 127 ESBL-producing Enterobacteriaceae isolated one year from inpatients and outpatients at public teaching São Paulo, Brazil, were submitted to analysis by PCR with specific primers for blaSHV, blaTEM blaCTX-M genes. From the isolates, 96 (75.6%) Klebsiella pneumoniae, 12 (9.3%) Escherichia coli, 8 (6.2%) Morganella morganii, 3 (2.3%) Proteus mirabilis, 2 (1.6%)...
It is known that Aeromonas spp. possess different chromosomal β-lactamase genes. Presence and phenotypic expression of bla TEM, SHV, CTX-M ESBL-encoding genes were investigated in environmental water isolates hydrophila jandaei. SHV was not observed, TEM gene verified 91% the isolates. Sequencing 10 fragments showed occurrence TEM-116.
Aeromonas are widely distributed in the aquatic environment, and considered to be emerging organisms that can produce a series of virulence factors. The present study was carried out sanitary sewage stabilization pond treatment system, located Lins, State São Paulo, Brazil. Most probable number applied for estimation genus Aeromonas. Colony isolation on blood agar ampicillin confirmed by biochemical characterization. species were isolated 72.4% influent samples, 55.2 48.3% effluent from...