A5022910906- Antibiotic Resistance in Bacteria
- Healthcare Regulation
- Bacterial biofilms and quorum sensing
- Antibiotic Use and Resistance
- Public Health in Brazil
- Antimicrobial Resistance in Staphylococcus
- Vibrio bacteria research studies
- Maternal and Neonatal Healthcare
- Antifungal resistance and susceptibility
- Pharmaceutical and Antibiotic Environmental Impacts
- Neonatal and Maternal Infections
- Enterobacteriaceae and Cronobacter Research
- Bacterial Identification and Susceptibility Testing
- Antibiotics Pharmacokinetics and Efficacy
- Fungal Infections and Studies
- Nosocomial Infections in ICU
- Global Maternal and Child Health
- Urinary Tract Infections Management
- Oral microbiology and periodontitis research
- Biosensors and Analytical Detection
- Health, Nursing, Elderly Care
- Bacteriophages and microbial interactions
- Pediatric health and respiratory diseases
- Escherichia coli research studies
- Infective Endocarditis Diagnosis and Management
Universidade Estadual de Londrina
2014-2024
Extraintestinal pathogenic Escherichia coli (ExPEC) isolates are responsible for many bloodstream infections. The aim of this study was to characterize E. isolated from the bloodstreams patients (n= 48) at University Hospital in Brazil. Epidemiological data were obtained through analysis medical records and laboratory tests. By PCR analysis, we investigated presence virulence factors (VFs), pathogenicity islands (PAIs), extended-spectrum β-lactamase (ESBL), phylogenetic classifications (A,...
Klebsiella pneumoniae is an important opportunistic pathogen associated with nosocomial and community-acquired infections. A wide repertoire of virulence antimicrobial resistance genes present in K. genomes, which can constitute extra challenges the treatment infections caused by some strains. Kp13 a multidrug-resistant strain responsible for causing large outbreak teaching hospital located Southern Brazil. produces carbapenemase (KPC-2) but unrelated to isolates belonging ST 258 11, main...
ABSTRACT New Delhi metallo-β-lactamase 1 (NDM-1) was first identified in Brazil Enterobacter hormaechei and Providencia rettgeri 2013. Here, we describe the case of NDM-1-producing Acinetobacter baumannii sequence type 25 isolated from urinary tract a 71-year-old man who died multiple complications, including A. infection. The NDM-1 gene detected by quantitative PCR, its confirmed presence an ∼100-kb plasmid.
Abstract Background An important virulence factor of Klebsiella pneumoniae is the production capsular polysaccharide (CPS), a thick mucus layer that allows for evasion host's defense and creates barrier against antibacterial peptides. CPS driven mostly by expression genes located in locus called cps , resulting structure used to distinguish between different serotypes (K types). In this study, we report unique genetic organization cluster from K. Kp13, clinical isolate recovered during large...
The production of KPC (Klebsiella pneumoniae carbapenemase) is the major mechanism resistance to carbapenem agents in enterobacterias. In this context, forty KPC-producing Enterobacter spp. clinical isolates were studied. It was evaluated activity antimicrobial agents: polymyxin B, tigecycline, ertapenem, imipenem and meropenem, performed a comparison methodologies used determine susceptibility: broth microdilution, Etest® (bioMérieux), Vitek 2® automated system (bioMérieux) disc diffusion....
Multidrug-resistant organisms (MDRO) are a great problem in hospitals, where thousands of people infected daily, with the occurrence high mortality rates, especially infections caused by Klebsiella pneumoniae carbapenemase-producing K. (KPC-producing Kpn). The challenge is to find new compounds that can control KPC producing-Kpn infections. aim this study was evaluate antibiotic activity F3d fraction produced Pseudomonas aeruginosa LV strain against clinical isolates KPC-producing Kpn....
To investigate the susceptibility pattern and molecular epidemiology of Acinetobacter baumannii isolates in two periods (1994-1996 2004-2007) Londrina University Hospital.Antimicrobial 150 A. was assessed by disc diffusion agar dilution methods. Genetic similarity amongst evaluated ERIC-PCR. Resistance to carbapenems increased from 2% (1994-1996) 73% (2004-2007). Thirty-eight clones were detected.The results study suggest that high prevalence carbapenem resistance organisms this institution...
Introduction: The mother plays a fundamental role in the constitution and regulation of her child's healthy microbiota, however, preterm newborns are separated from their mothers soon after birth transferred to Neonatal Intensive Care Units, being exposed constant risk for development multidrug-resistant microorganisms’ infections. aim this study was explore microorganism colonization hospitalized babies neonatal unit context.
 Methodology: A prospective case conducted with Unit at...
In this work, two accurate and sensitive real-time polymerase chain reaction (PCR) assays to differentiate pathogenic Cryptococcus gattii sensu lato ( s.l. ) C. neoformans targeting the intergenic spacer 1 (IGS1) region from rDNA locus were developed. Specific primers designed based on their IGS1 sequence analyses optimal PCR showed that dissociation curves generated different melting peaks, at 82.8 84.2ºC for , respectively. No amplifications observed in negative template control. The...
The prompt and accurate identification of the etiological agents viral respiratory infections is a critical measure in mitigating outbreaks. In this study, we developed clinically evaluated novel melting-curve-based multiplex real-time PCR (M-m-qPCR) assay targeting RNA-dependent RNA polymerase (RdRp) nucleocapsid phosphoprotein N SARS-CoV-2, Matrix protein 2 Influenza A virus, RdRp domain L from Human Respiratory Syncytial Virus, polyprotein Rhinovirus B genes. analytical performance...
Introduction: The emergence of Klebsiella pneumoniae carbapenemase (KPC)-producing K. (KPC-Kpn) isolates is attracting significant attention in nosocomial infection settings. the main pathogen that harbours blaKPC genes.
 Methodology: This study evaluated 54 carbapenem-resistant from patients hospitalized at University Hospital Londrina, between July 2009 and 2010. were phenotypically screened for production submitted genotypic confirmation by polymerase chain reaction (PCR) KPC,...
Justificativa e Objetivo: Infecções da corrente sanguínea por Staphylococcus aureus constituem uma das principais causas de morbidade mortalidade em todo mundo. O tratamento infecções S. é complicado pela elevada prevalência resistência a antimicrobianos. Compreender epidemiologia os padrões deste microrganismo um ponto crítico para prescrição empírica adequada Desta maneira, este estudo teve objetivo avaliar evolução antimicrobiana num período quinze anos. Metodologia: Foram analisados...
Infecções associadas aos cuidados de saúde (IRAS) constituem importante preocupação à segurança do paciente ao redor mundo. Pneumonia associada ventilação mecânica (VAP) é a principal causa morte entre IRAS, com mortalidade 15 70%, dependendo da população pacientes. O Centro Controle e Prevenção Doenças (CDC) recomendou que todas as UTIs implementassem um bundle para reduzir taxa PAV. Os objetivos presente estudo foram analisar o efeito pacote intervenções controle infecção, educação, taxas...
A retrospective study of pregnant women seen at the University Hospital Londrina, Paraná, Brazil was performed to determine prevalence Group B Streptococcus (GBS) vaginal-rectal colonization, and GBS susceptibility for antimicrobials used in intrapartum antibiotic prophylaxis. swab collected from 2,901 between 35 37 weeks gestation. Of these, 528 (18.2%) had a positive culture GBS, 0.4%, 10.2% 10% isolates were resistant penicillin, erythromycin clindamycin, respectively. These results...
A retrospective study of pregnant women seen at the University Hospital Londrina, Paraná, Brazil was performed to determine prevalence Group B Streptococcus (GBS) vaginal-rectal colonization, and GBS susceptibility for antimicrobials used in intrapartum antibiotic prophylaxis.A swab collected from 2,901 between 35 37 weeks gestation.Of these, 527 (18.2%) had a positive culture GBS, 0.4%, 10.2% 10% isolates were resistant penicillin, erythromycin clindamycin, respectively.These results...
As infecções graves causadas pelo gênero Candida têm se tornado um desafio na questão diagnóstica, no intuito de detectar e identificar o agente etiológico forma ágil, precisa padronizada nos laboratórios clínicos. A predição da susceptibilidade aos antifúngicos, bem como a necessidade geração dados epidemiológicos reforçam importância identificação rotineira adequada das espécies leveduras envolvidas em infecções. Dentre as 200 já descritas, C. albicans, parapsilosis, tropicalis, glabrata,...
Staphylococcus aureus is a major cause of wide diversity infections in humans, and the expression Panton-Valentine Leukocidin (PVL) has been associated with severe clinical syndromes. The present study aimed to investigate prevalence PVL-encoding genes S. isolated from samples inpatients invasive teaching hospital Southern Brazil. Furthermore, phenotypic genotypic characteristics bacterial isolates were analyzed. A total 98 recovered different body sites characterized according their...