ABSTRACT The gp43 glycoprotein is an immune-dominant antigen in patients with paracoccidioidomycosis (PCM). It protective against murine PCM and a putative virulence factor. gene of Paracoccidioides brasiliensis B-339 located 1,329-bp DNA fragment that includes two exons, 78-bp intron, leader peptide-coding region 105 bp. Polymorphism has been suggested by the occurrence, same isolate or among different fungal samples, isoforms distinct isoelectric points. In present study we aligned...

Phenotypic variability in pathogenic fungi has long been correlated with virulence, but speci c genetic and molecular mechanisms are only recently being unraveled.Fungal morphogenesis, re ecting the expression of several regulated genes, capacity rising forms or phases to cause disease focused on at XIVth Congress International Society for Human Animal Mycology.Three experimental models have discussed.In Cryptococcus neoformans, phenotypic switching represents controlled programmed changes...

The emergence of multiresistant strains bacteria reinforces the need to search for new compounds able combat resistant organisms. Medicinal plants are a great resource bioactive substances, providing possibility obtaining molecules with potential antimicrobial activity. aim present study is evaluation antibacterial activity extracts and alkaloids isolated from root bark Zanthoxylum tingoassuiba A. St.-Hil., Rutaceae, against four clinical isolates Staphylococcus aureus ATCC 25923....

Zanthoxylum tingoassuiba essential oil (EO) is a complex mixture of organic compounds among which methyl-N-methylanthranilate and sesquiterpene alcohol alpha-bisabolol represent the main compounds. The antimicrobial activity was studied against bacteria fungi cells by diffusion disk method significant observed S. aureus, aureus isolated multi-resistant dermathophyte fungi. Essential from loaded into DPPC multilamellar liposomes (MLV) successfully produced through thin film hydration with...

Zanthoxylum tingoassuiba essential oil (EO) is a complex mixture of organic compounds among which methyl-N-methylanthranilate and sesquiterpene alcohol α-bisabolol represent the main compounds. The antimicrobial activity was studied against bacteria fungi cells by diffusion disk method significant observed S. aureus, aureus isolated multi-resistant dermathophyte fungi. Essential from loaded into DPPC multilamellar liposomes (MLV) successfully produced through thin film hydration with mean...

To determine the profiles of susceptibility to antifungal and genotypes clinical isolates Cryptococcus in Bahia, Brazil, 62 were collected from cases meningitis period 2006 2010. Their susceptibilities fluconazole, itraconazole, amphotericin B 5-flucytosine determined by broth microdilution technique described Clinical Laboratory Standards Institute genotyping URA5 gene was accomplished restriction fragment length polymorphism. C. neoformans accounted for 79% identified yeast gattii...

Candida albicans(C. albicans) is the major infectious agent of oral candidiasis, and both innate immunity cell-mediated immune response participate in control fungal infections. The aim this study was to correlate clinical forms candidiasis with number colony forming units (CFU) C. albicans saliva characterize T cell patients candidiasis. Participants included 75 subjects: 36 lesions 39 without A 2-ml sample collected from all subjects for microbiological analysis. Cytokine levels were...

Procedures for the easy preparation of a series phytyl phenolipids were developed, yielding esters caffeic, protocatechuic, homoprotocatechuic and dihydrocaffeic acids in high yields (70-95%). Preliminary characterization showed that these new compounds have similar lipo-solubility comparable radical scavenging capacity to natural membrane antioxidant -tocopherol. Cyclic voltammetry analysis lower anodic peak potentials when compared with parental phenolic electron transfer followed an...

Biofilms are a virulence factor for Candida albicans, common pathogen in human fungal infections, making them resistant to many commercial antifungals. Therefore, the discovery of compounds that inhibit and eradicate biofilms is priority. As thiosemicarbazones have had their effect on little explored, this study investigated inhibitory eradication activity 30 analogues against C. albicans biofilms. After initial screening, four were selected compound 28 emerged as most potent with BIC50 at...

ABSTRACT J-domain (DnaJ) proteins, of the Hsp40 family, are essential cofactors their cognate Hsp70 chaperones, besides acting as independent chaperones. In present study, we have demonstrated presence Mdj1, a mitochondrial DnaJ member, not only in mitochondria, where it is apparently sorted, but also cell wall Paracoccidioides brasiliensis , thermodimorphic pathogenic fungus. The molecule (PbMdj1) was localized to fungal yeast cells using both confocal and electron microscopy flow...

Respiratory infection is very common in patients suffering from cystic fibrosis (CF). However, the antimicrobial resistance rate of isolates CF not often documented. In this study, 279 respiratory specimens 146 were prospectively collected July to December 2006. Microbiological cultures and susceptibility tests most frequently isolated bacteria performed. Sputum oropharyngeal swabs processed for culture. During study period, 50% harbored Staphylococcus aureus, 35% Pseudomonas aeruginosa,...

Cryptococcosis is an infectious disease of worldwide distribution, caused by encapsulated yeasts belonging to the phylum Basidiomycota. The genus Cryptococcus includes several species distributed around world. C. gattii/neoformans complex largely responsible for most cases cryptococcosis. However, clinical series have been published infections Papiliotrema (Cryptococcus) laurentii and Naganishia albida (Cryptococcus albidus), among other related genera. Here, we examined pathogenic potential...

The multifactorial etiology of gastroenteritis emphasizes the need for different laboratory methods to identify or exclude infectious agents and evaluate severity diarrheal disease. To diagnose in diarrheic children some fecal markers associated with intestinal integrity. study group comprised 45 disease, tested enteropathogens malabsorption markers, 76 whose feces were used fat evaluation by traditional acid steatocrit tests. We observed acute diarrhea 80% persistent 20%. Of individuals...

Ensaios para avaliar o potencial antibacteriano de Rhizophora mangle (mangue-vermelho), coletada no município Conde, Bahia, foram realizados com cepas bacterianas Gram-positivas (Staphylococcus aureus ATCC 6538 e Micrococcus luteus 9341) Gram-negativas (Echerichia coli 10536, Salmonella Cholerea-suis 10708, Klebsiela pneumoniae 700603 Pseudomonas aeruginosa 15442). A atividade antibacteriana foi avaliada pelos métodos difusão em disco concentração inibitória mínima (CIM), os resultados...

A doença periodontal (DP) é descrita como um conjunto de processos inflamatórios e infecciosos que acomete os tecidos periodontais, etiologia multifatorial, localização sítiodependente considerada pela Organização Mundial Saúde uma das duas principais enfermidades risco para a saúde bucal. Desencadeada perpetuada por bactérias anaeróbias Gram-negativas, cuja resistência antibioticoterapia convencional antissépticos bucais exigem busca novos métodos coadjuvantes ao tratamento clínico...

In this work, two accurate and sensitive real-time polymerase chain reaction (PCR) assays to differentiate pathogenic Cryptococcus gattii sensu lato ( s.l. ) C. neoformans targeting the intergenic spacer 1 (IGS1) region from rDNA locus were developed. Specific primers designed based on their IGS1 sequence analyses optimal PCR showed that dissociation curves generated different melting peaks, at 82.8 84.2ºC for , respectively. No amplifications observed in negative template control. The...

Natural preservative substances are an efficient and economic alternative, because the aromatical plants rich spices in essential oils characterized by a notable gotten from antimicrobial activity, for this reason, its derived products can be used to delay or inhibit growth of microorganisms. The objective study was evaluate antibacterial activity clove, limon, oregano (Origanum vulgare ssp. Hirtum) garlic several species bacteria like Staphylococcus aureus Escherichia coli isolated vongole...

Abstract A LON gene homologue from the human pathogen Paracoccidioides brasiliensis ( PbLON ) has been cloned, sequenced and characterized. It encodes a putative ATP‐dependent proteinase Lon, which in Saccharomyces cerevisisae (PIM1) is heat‐inducible protein involved degradation of abnormal or short‐lived proteins mitochondria. The ORF within 3369 bp fragment interrupted by two introns located 3′segment. 5′ 3′ regions flanking contain sequences resemble known transcription elements. Several...

Candida albicans (CA) has been identified as the major opportunistic pathogen in immunosuppressed patients. Most of currently available drugs are either highly toxic or becoming ineffective against resistant strains. An approach to overcome this burden relies on azole derivatives with increased potency and selectivity. Aiming at shedding some light structural chemical features that important for antifungal activity derivatives, classical 2D QSAR hologram (HQSAR) studies were performed a...

Com o aumento da resistência bacteriana aos antibióticos disponíveis, tornou-se imprescindível a busca por novos fármacos ou protótipos. Os metabólitos secundários produzidos alguns vegetais como cumarinas, alcaloides e terpenoides podem apresentar várias atividades biológicas, dentre elas, atividade antibiótiotica. O objetivo deste trabalho foi avaliar antimicrobiana in vitro, pelo método de difusão em disco, das diferentes partes duas espécies pertencentes à família Rutaceae coletadas na...