A5085992622- Trypanosoma species research and implications
- Research on Leishmaniasis Studies
- Bacterial biofilms and quorum sensing
- Antimicrobial Resistance in Staphylococcus
- Synthesis and Biological Evaluation
- Antifungal resistance and susceptibility
- Bacterial Identification and Susceptibility Testing
- Neonatal and Maternal Infections
- Fungal Infections and Studies
- Antibiotic Resistance in Bacteria
- Streptococcal Infections and Treatments
- Antimicrobial Peptides and Activities
- Insect Pest Control Strategies
- Nail Diseases and Treatments
- Toxin Mechanisms and Immunotoxins
- Bee Products Chemical Analysis
- Helminth infection and control
- SARS-CoV-2 and COVID-19 Research
- Essential Oils and Antimicrobial Activity
- Parasites and Host Interactions
- Maternal and Neonatal Healthcare
- Pneumocystis jirovecii pneumonia detection and treatment
- SARS-CoV-2 detection and testing
- Toxoplasma gondii Research Studies
- Healthcare during COVID-19 Pandemic
Universidade Estadual de Londrina
2016-2025
Universidade Estadual do Norte do Paraná
2021
New York University
2007
Clinics Hospital of Ribeirão Preto
2006
Universidade Federal de São Paulo
1996-2000
Plasmodium sporozoites make a remarkable journey from the skin, where they are deposited by an infected Anopheline mosquito, to liver, invade hepatocytes and develop into exoerythrocytic stages. Although much work has been done elucidate molecular mechanisms which hepatocytes, little is known about interactions between host parasite before sporozoite enters blood circulation. It always assumed that rapidly exit injection site, making their with at this brief difficult study. Using...
The 43,000-Da glycoprotein (gp43) of Paracoccidioides brasiliensis is an immunodominant antigen for antibody-dependent and immune cellular responses in patients with paracoccidioidomycosis. In order to identify the peptide epitopes involved immunological reactivities gp43 obtain highly specific recombinant molecules diagnosis infection, genomic cDNA clones representing entire coding region were sequenced. open reading frame was found a 1,329-base pair fragment 2 exons interrupted by intron...
Silver nanoparticles (AgNPs) have been extensively studied because of their anti-microbial potential. Here, we evaluated the effect biologically synthesized silver (AgNP bio ) alone and in combination with fluconazole (FLC) against planktonic cells biofilms FLC-resistant Candida albicans . AgNP exhibited a fungicidal effect, minimal inhibitory concentration (MIC) ranging from 2.17 to 4.35 μg/ml. The FLC reduced MIC around 16 64 times all C. There was no significant on biofilm cells. However,...
American cutaneous leishmaniasis is a zoonotic disease caused by protozoans of the genus Leishmania. The treatment unsatisfactory, thus, much research effort has been focused on investigating new compounds with lower collateral effects to patients and derived from low-cost sources, such as natural products. In present study, we evaluated in vitro directly effect flavonoid quercetin against Leishmania (Viannia) braziliensis. Quercetin inhibited proliferation promastigote forms at all tested...
The increase in multidrug-resistant microorganisms represents a global threat requiring the development novel strategies to fight bacterial infection. This study aimed assess effect of silver nanoparticles (bio-AgNPs) on growth, biofilm formation, production virulence factors, and expression genes related quorum-sensing (QS) system P. aeruginosa PAO1 PA14. Biofilm formation assays were performed with bio-AgNPs. RT-qPCR was carried out determine bio-AgNPs QS regulatory lasI, lasR, rhlI, rhlR,...
Leukotrienes are important mediators of inflammatory responses. In this study, we investigated the effect absence 5-lipoxygenase (5-LO)-derived leukotrienes on levels cytokines, nitric oxide (NO) and iNOS expression in cardiac tissue mice infected with Trypanosoma cruzi, agent Chagas' disease. NO is a key mediator parasite killing experimentally T. previous studies have suggested that leukotrienes, such as LTB(4), induces synthesis cruzi-infected macrophages plays relevant role NO-dependent...
Most Candida spp. infections are associated with biofilm formation on host surfaces. Cells within these communities display a phenotype resistant to antimicrobials and defenses, so biofilm-associated difficult treat, representing source of reinfections. The present study evaluated the effect eugenol adherence properties capacity dubliniensis tropicalis isolated from oral cavity HIV-infected patients. All isolates were able form biofilms different substrate Eugenol showed inhibitory activity...
Streptococcus agalactiae or Group B Streptococci (GBS) have the ability to access various host sites, which reflects its adaptability different environments during course of infection. This adaptation is due expression virulence factors that are involved with survival, invasion and bacterial persistence in host. study aimed characterize GBS isolates from women reproductive age seen at University Hospital Londrina, according capsular typing, genetic relatedness, antimicrobial susceptibility...
Streptococcus agalactiae (group B streptococci (GBS)) is an important infections agent in newborns associated with maternal vaginal colonization. Intrapartum antibiotic prophylaxis GBS-colonized pregnant women has led to a significant reduction the incidence of early neonatal infection various geographic regions. However, this strategy may lead resistance selecting among GBS, indicating need for new alternatives prevent bacterial transmission and even treat GBS infections. This study...
Candida species are some of the most common causes fungal infection worldwide. The limited efficacy clinically available antifungals warrants search for new compounds treating candidiasis. This study evaluated effect condensed tannin-rich fraction (F2 fraction) Stryphnodendron adstringens on in vitro and vivo growth tropicalis, yeast adhesion properties. F2 exhibited a fungistatic with minimum inhibitory concentration ranging from 0.5 to 8.0 μg/mL. A significant reduction biofilm mass was...
The fact that drugs currently used in the treatment of Leishmania are highly toxic and associated with acquired resistance has promoted search for new therapies treating American tegumentary leishmaniasis (ATL). In this study, BALB/c mice were injected hind paw (Leishmania) amazonensis subsequently treated a combination nitric oxide (NO) donor (cis-[Ru(bpy) 2imN(NO)](PF6)3) (Ru-NO), given by intraperitoneal injection, oral Brazilian propolis 30 days. Ru-NO reached center lesion increased NO...
Abstract Background To overcome the problem of increasing drug resistance, traditional medicines are an important source for potential new anti-malarials. Caesalpinia pluviosa , commonly named "sibipiruna", originates from Brazil and possess multiple therapeutic properties, including anti-malarial activity. Methods Crude extract (CE) was obtained stem bark by purification using different solvents, resulting in seven fractions. An MTT assay performed to evaluate cytotoxicity MCF-7 cells. The...
ABSTRACT American trypanosomiasis, or Chagas' disease, is caused by Trypanosoma cruzi and affects around 15 million people throughout the continent. The available treatment based on two nitroheterocyclic drugs, nifurtimox benznidazole, both only partially effective toxic. In this context, new drugs must be found. our previous work, tetrahydro-β-carboline compound N -butyl-1-(4-dimethylamino)phenyl-1,2,3,4-tetrahydro-β-carboline-3-carboxamide, named C4, showed a potent in vitro trypanocidal...
Introduction: Host colonization by Candida species is an important predisposing factor to candidiasis, which seems be more frequent in human immunodeficiency virus (HIV)-infected patients. Knowledge about the distribution, antifungal susceptibility, and virulence of oral isolates for effective management candidiasis. Methodology: Oral rinses were collected from 242 HIV-infected patients without clinical evidence candidiasis seen at AIDS referral center Londrina, Brazil. Species identified...
This study aimed to characterize the main mechanisms of acquired antimicrobial resistance 103 multidrug-resistant Acinetobacter baumannii isolated from bloodstream 2006 2016 a hospital in Londrina, Brazil. All isolates were identified as A. by amplification blaOXA-51-like and rpoB genes. Mortality was observed majority (81.6%) patients. High non-susceptibility rates (100.0-10.7%) obtained for evaluated antimicrobials, including colistin, polymyxin B, tigecycline, most classified extensively...
Background. Staphylococcus aureus can asymptomatically colonize the human anterior nares and skin, nasal colonization by this bacterium represents a potential risk for development of invasive infections. The aim study was to determine prevalence S. carriage among healthcare workers students attending university hospital characterize isolates phenotypically molecularly. Methods. A cross-sectional performed with 324 volunteers. Cultures from samples were obtained characterized according their...
Since ancient times, plants have been used in folk medicine to treat different diseases. Plants offer exceptional chemical diversity with a wide range of biological activities, and therefore the most promising sources for discovery development drugs, including antimicrobial agents. This study reports antibacterial effect geraniol (GER), alone combination biogenic silver nanoparticles (bioAgNPs), produced using aqueous extract Trichilia catigua bark, against planktonic sessile cells...
This study reports the construction, expression, and purification of synthetic SARS-CoV-2 spike (S) nucleoprotein (N) containing immunodominant epitopes. The pET28aS_epit construct included epitopes 287–317, 402, 507, 524–598, 601–640, while pET28aN_epit residues 42–62, 153–172, 355–401. Commercial sequences both proteins were used as controls. four constructs expressed using Escherichia coli BL21(DE3) star strain at 37 °C. results show that S protein insoluble, unlike N constructs. Both...