A5078625205- Toxoplasma gondii Research Studies
- Psoriasis: Treatment and Pathogenesis
- Immune Cell Function and Interaction
- Cancer Immunotherapy and Biomarkers
- Cytokine Signaling Pathways and Interactions
- Cytomegalovirus and herpesvirus research
- Cancer Cells and Metastasis
- Research on Leishmaniasis Studies
- Immunotherapy and Immune Responses
- Immune Response and Inflammation
- IL-33, ST2, and ILC Pathways
- SARS-CoV-2 and COVID-19 Research
- Immune cells in cancer
- T-cell and B-cell Immunology
- COVID-19 Clinical Research Studies
- NF-κB Signaling Pathways
- Hepatitis Viruses Studies and Epidemiology
- HER2/EGFR in Cancer Research
- Reproductive System and Pregnancy
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Parasites and Host Interactions
- Herpesvirus Infections and Treatments
- Parasitic Infections and Diagnostics
- Heme Oxygenase-1 and Carbon Monoxide
- Liver Diseases and Immunity
Universidade de São Paulo
2013-2023
Fundação Oswaldo Cruz
2022
Clinics Hospital of Ribeirão Preto
2009-2016
Cedars-Sinai Medical Center
2012-2013
The aggressiveness of invasive ductal carcinoma (IDC) the breast is associated with increased IL17 levels. Studying role in tumor pathogenesis, we found that metastatic primary tumor-infiltrating T lymphocytes produced elevated levels IL17, whereas neutralization inhibited growth and prevented migration neutrophils cells to secondary disease sites. Tumorigenic promote progression, producing CXCL1, MMP9, VEGF, TNFα, their depletion suppressed growth. IL17A also induced IL6 CCL20 production...
Background: Human postnatal stem cells have been identified in periodontal ligaments (PDLs). In this study, the vitro biologic properties of CD105 + enriched cell subsets from PDLs harvested deciduous (DePDL) and permanent (PePDL) teeth are comparatively assessed. Methods: PDL tissue was obtained 12 (six primary six permanent) which CD34 − CD45 were isolated by magnetic sorting. To identify quantitatively compare markers, DePDL PePDL assessed for CD166 surface antigen expression flow...
Immune evasion by tumors includes several different mechanisms, including the inefficiency of antigen presenting cells (APCs) to trigger anti-tumor T cell responses. B lymphocytes may display a pro-tumoral role but can also be modulated function as lymphocytes, capable triggering anti-cancer immune While dendritic cells, DCs, are best APC population activate naive DCs or their precursors, monocytes, frequently tumors, displaying tolerogenic phenotype in cancer patients. In patients with...
Breast cancer is a leading cause of neoplasia‐associated death in women worldwide. Regulatory T (Treg) and Th17 cells are enriched within some tumors, but the role these play invasive ductal carcinoma (IDC) breast unknown. We show that CD25 + CD4 from PBMCs tumor express high levels Foxp3, GITR, CTLA‐4, CD103, indicating tumor‐infiltrating Treg functional possibly recruited by CCL22. Additionally, we observed upregulation Th17‐related molecules (IL‐17A, RORC, CCR6) IL‐17A produced CD8...
Abstract Both T cells and B have been shown to be generated after infection with SARS-CoV-2 yet protocols or experimental models study one the other are less common. Here, we generate a chimeric protein (SpiN) that comprises receptor binding domain (RBD) from Spike (S) nucleocapsid (N) antigens SARS-CoV-2. Memory CD4 + CD8 specific for SpiN could detected in blood of both individuals vaccinated Coronavac vaccine COVID-19 convalescent donors. In mice, elicited strong IFN-γ response by high...
Abstract The transcription factor B lymphocyte–induced maturation protein-1 (Blimp-1) plays important roles in embryonic development and immunity. Blimp-1 is required for the differentiation of plasma cells, mice with T cell–specific deletion (Blimp-1CKO mice) develop a fatal inflammatory response colon. Previous work demonstrated that lack CD4+ CD8+ cells leads to intrinsic functional defects, but little known about role regulating Th vivo their contribution chronic intestinal inflammation...
The current COVID-19 vaccines protect against severe disease, but are not effective in controlling replication of the Variants Concern (VOCs). Here, we used existing pre-clinical models and moderate to evaluate efficacy a Spike-based DNA vaccine (pCTV-WS) for protection different VOCs. Immunization transgenic (K18-hACE2) mice hamsters induced significant levels neutralizing antibodies (nAbs) Wuhan Delta isolates, Gamma Omicron variants. Nevertheless, pCTV-WS offered all Consistently, lung...
Neospora caninum is an intracellular parasite that causes major economic impact on cattle raising farms, and infects a wide range of warm-blooded hosts worldwide.Innate immune mechanisms lead to protection against this are still unknown.In order investigate whether myeloid differentiation factor 88 (MyD88) required for resistance N. caninum, genetically deficient mice (MyD88 À/À ) wild type littermates were infected with live tachyzoites the infection was evaluated.We found sub-lethal...
Nod2 belongs to the nucleotide-binding domain leucine-rich repeat family of proteins and senses bacterial cell wall components initiate innate immune responses against various pathogens. Recently, it has been reported that T-cell-intrinsic expression promotes host defense Toxoplasma gondii infection by inducing type 1 immunity. Here, we present results demonstrate does not play a role in T. infection. Nod2-deficient mice were fully capable Th1 did show enhanced susceptibility Upon TCR...
The granulomatous lesion resulting from infection with the fungus Paracoccidioides brasiliensis is characterized by a compact aggregate of mature cells, surrounded fibroblast- and collagen-rich content. Granuloma formation requires signaling elicited inflammatory molecules such as members interleukin-1 family. Two this family have been thoroughly studied, namely IL-1α IL-1β. In study, we addressed mechanisms underlying secretion its functional role on host resistance to fungal infection. We...
Toxoplasma gondii induces a potent IL-12 response early in infection that results IFN-γ-dependent control of parasite growth. It was previously shown T. soluble tachyzoite antigen (STAg) injected 48 hr before intraperitoneal reduces lipoxin A4 and 5-lipoxygenase (5-LO)-dependent systemic IFN-γ production as well hepatic immunopathology. This study investigated the ability STAg-pretreatment to fatal intestinal pathology develops C57BL/6 mice orally infected with 100 cysts. prolonged animals'...
Adenosine is an endogenously released purine nucleoside that signals through four widely expressed G protein-coupled receptors: A1, A2A, A2B, and A3. Of these, A2AR recognized as mediating major adenosine anti-inflammatory activity. During cutaneous leishmaniasis, induces immunosuppression, which promotes the establishment of infection. Herein, we demonstrated signaling exploited by Leishmania infantum parasites, etiologic agent causes Visceral Leishmaniasis, to successfully colonize...
CCR5, an important receptor related to cell recruitment and inflammation, is expressed during experimental Toxoplasma gondii infection. However, its role in the immunopathology of toxoplasmosis not clearly defined yet. Thus, we inoculated WT CCR5-/- mice with a sub lethal dose parasite by oral route. were extremely susceptible infection, presenting higher load lower tissue expression IL-12p40, IFN-γ, TNF, IL-6, iNOS, Foxp3, T-bet, GATA-3 PPARα. Although both groups presented inflammation...
The prostaglandin, 15-deoxy<mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" id="M1"><mml:mrow><mml:msup><mml:mrow><mml:mi mathvariant="normal">Δ</mml:mi></mml:mrow><mml:mrow><mml:mn mathvariant="normal">12,14</mml:mn></mml:mrow></mml:msup></mml:mrow></mml:math>-prostaglandin J 2 (15d-PGJ ), is a lipid mediator that plays an important role in the control of chronic inflammatory disease. However, prostanoid rheumatoid arthritis (RA) not well determined. We demonstrated therapeutic...
A striking feature of human visceral leishmaniasis (VL) is chronic inflammation in the spleen and liver, VL patients present increased production levels multiple inflammatory mediators, which contribute to tissue damage disease severity. Here, we combined an experimental model with transcriptional profile demonstrate that TLR4-IFN-β pathway regulates process associated asymptomatic form disease. Tlr4-deficient mice harbored fewer parasites their liver than wild-type mice. TLR4 deficiency...
This is a case series study to evaluate immunological markers associated with schistosomiasis advanced fibrosis, including 69 patients from an endemic area the State of Sergipe and Hepatology Service University Hospital in Sergipe, Brazil. Hepatic fibrosis was classified based on Niamey protocol for ultrasonography (US). Immune response Schistosoma mansoni antigens evaluated by stimulating peripheral blood mononuclear cells (PBMCs) these either adult worm (SWAP-10 μg/ml) or egg (SEA-10...
Sepsis is a severe syndrome that arises when the host response to an insult exacerbated, leading organ failure and frequently death. How chronic infection causes prolonged Th1 expansion affects course of sepsis unknown. In this study, we showed mice chronically infected with Toxoplasma gondii were more susceptible induced by cecal ligation puncture (CLP). Although T. gondii-infected exhibited efficient control bacterial burden, they increased mortality compared groups. Mechanistically,...
Monocytes play key roles in the maintenance of homeostasis and control infection. are recruited from bone marrow to inflammatory sites essential for antimicrobial activity limit tissue damage promote adaptive T cell responses. Here, we investigated role NFAT1 regulation Ly6Chi monocyte recruitment CNS upon T. gondii We show that NFAT-1-deficient monocytes unable migrate gondii-infected mice. Moreover, NFAT1-/- mice highly susceptible chronic infection due a failure parasite replication CNS....
In many infectious diseases, the pathogen-induced inflammatory response could result in protective immunity that should be regulated to prevent tissue damage and death. fact, Trypanosoma cruzi infection, innate immune perfectly controlled avoid significant lesions Here, we investigate role of Blimp-1 expression T cells resistance T. infection. Therefore, using mice with deficiency (CKO) determined its controlling parasites growth during acute phase Infection ablation resulted failure...