A5000765759- SARS-CoV-2 and COVID-19 Research
- COVID-19 Clinical Research Studies
- Microtubule and mitosis dynamics
- Trypanosoma species research and implications
- Research on Leishmaniasis Studies
- Cellular Mechanics and Interactions
- Immunotherapy and Immune Responses
- vaccines and immunoinformatics approaches
- Animal Virus Infections Studies
- Genomics and Phylogenetic Studies
- Viral Infections and Immunology Research
- Vector-borne infectious diseases
- Microbial Community Ecology and Physiology
- Platelet Disorders and Treatments
- RNA Interference and Gene Delivery
- Genomic variations and chromosomal abnormalities
- Virology and Viral Diseases
- Blood groups and transfusion
- Toxoplasma gondii Research Studies
- Hepatitis B Virus Studies
- Mosquito-borne diseases and control
- Bacteriophages and microbial interactions
- Cancer Treatment and Pharmacology
- Blood properties and coagulation
- Healthcare Regulation
Universidade Federal de Minas Gerais
2020-2025
Fundação Oswaldo Cruz
2018-2024
Universidade de São Paulo
2010-2024
The University of Texas Health Science Center at San Antonio
2024
Institute for Neurodegenerative Disorders
2024
Fundação Faculdade de Medicina
2021-2022
University of California, Davis
2020-2021
University of Massachusetts Chan Medical School
2021
Weatherford College
2021
Centro de Ingeniería Genética y Biotecnología
2008
Abstract Both T cells and B have been shown to be generated after infection with SARS-CoV-2 yet protocols or experimental models study one the other are less common. Here, we generate a chimeric protein (SpiN) that comprises receptor binding domain (RBD) from Spike (S) nucleocapsid (N) antigens SARS-CoV-2. Memory CD4 + CD8 specific for SpiN could detected in blood of both individuals vaccinated Coronavac vaccine COVID-19 convalescent donors. In mice, elicited strong IFN-γ response by high...
Trypanosoma cruzi is the causative agent of Chagas disease, a neglected tropical disease that affects approximately 6 to 8 million people and for which there no effective treatment or vaccine. The parasite expresses family surface proteins, named trans-sialidases, responsible transferring sialic acid from host glycoconjugates mucins.
The microtubule-associated protein, doublecortin-like kinase 1 (DCLK1), is highly expressed in a range of cancers and prominent therapeutic target for inhibitors. physiological roles DCLK1 activity how it regulated remain elusive. Here, we analyze the role mammalian regulating microtubule binding. We found that autophosphorylates residue within its C-terminal tail to restrict prevent aberrant hyperphosphorylation microtubule-binding domain. Removal or mutation this causes an increase...
Abstract Immunization with the Amastigote Surface Protein-2 (ASP-2) and Trans -sialidase (TS) antigens either in form of recombinant protein, encoded plasmids or human adenovirus 5 (hAd5) confers robust protection against various lineages Trypanosoma cruzi . Herein we generated a chimeric protein containing most immunogenic regions for T B cells from TS ASP-2 (TRASP) evaluated its immunogenicity comparison our standard protocol heterologous prime-boost using hAd5. Mice immunized TRASP...
Severe thrombocytopenia and increased vascular permeability are two major characteristics of dengue hemorrhagic fever (DHF). An immune mechanism due to platelet destruction appears be operative in patients with DHF (see Saito et al., 2004, Clin Exp Immunol 138: 299–303; Mitrakul, 1979, Am J Trop Med Hyg 26: 975–984; Boonpucknavig, 28: 881–884). The interim data randomized placebo controlled trials ( N = 47) meeting WHO criteria for (DHF) severe (platelets ≤ 50,000/mm 3 ) reveal that the...
P. vivax-infected Retics (iRetics) express human leukocyte antigen class I (HLA-I), are recognized by CD8+ T cells and killed granulysin (GNLY) granzymes. However, how Plasmodium infection induces MHC-I expression on is unknown. In addition, whether GNLY helps control in vivo has not been studied. Here, we examine these questions using rodent with the yoelii 17XNL strain, which tropism for Retics. Infection caused extramedullary erythropoiesis, reticulocytosis expansion of CD8+CD44+CD62L-...
Allergy is on the rise worldwide. Asthma, food allergy, dermatitis, and systemic anaphylaxis are amongst most common allergic diseases. The association between allergy altered behavior patterns has long been recognized. molecular cellular pathways in bidirectional interactions of nervous immune systems now starting to be elucidated. In this paper, we outline consequences diseases, especially asthma, neural activity modulation responses.
The persistent circulation of SARS-CoV-2 represents an ongoing global threat due to the emergence new viral variants that can sometimes evade immune system previously exposed or vaccinated individuals. We conducted a follow-up study adult individuals had received inactivated vaccine, evaluating antibody production and neutralizing activity over period 6 months. In addition, we performed mice immunization with SARS-CoV-2, evaluated response pathological outcomes against Gamma Zeta variant...
Aim: To develop, characterize and evaluate an oil/water nanoemulsion with squalene (CTVad1) to be approved as adjuvant for the SpiN COVID-19 vaccine clinical trials. Materials & methods: Critical process parameters (CPPs) of CTVad1 were standardized meet critical quality attributes (CQAs) human use. SpiN-CTVad1 submitted physicochemical, stability, in vitro vivo studies. Results conclusion: All CQAs met production process. SpiN- induced high levels antibodies specific cellular responses...
Abstract Background APP duplications are a rare form of familial Alzheimer’s disease (AD). Research has shown variability in clinical presentation with full duplications. There is limited information on those partial duplications, especially underrepresented minorities. Methods A 64 y/o Hispanic male presented 2.5 year history anxiety, restlessness, coarsening behavior, cognitive and functional decline, motor changes. We present findings from neuropsychological evaluation, neurological exam,...
Halophile-specific enzymes have wide-ranging industrial and commercial applications. Despite their importance, there is a paucity of available halophile whole-genome sequences. Here, we report the draft genome sequences 16 diverse salt-tolerant strains bacteria archaea isolated from variety high-salt environments.
The nucleocapsid (N) and the receptor binding domain (RBD) of Spike (S) proteins elicit robust antibody T cell responses either in vaccinated or COVID-19 convalescent individuals. We generated a chimeric protein that comprises sequences RBD from S N antigens (SpiN). SpiN was highly immunogenic elicited strong IFNγ response cells high levels antibodies to inactivated virus, but no neutralizing antibodies. Importantly, hamsters human Angiotensin Convertase Enzyme-2-transgenic mice immunized...
Abstract The microtubule-associated protein (MAP), doublecortin-like kinase 1 (DCLK1), is highly expressed in a range of cancers and prominent therapeutic target for the development inhibitors. However, physiological roles its activity how DCLK1 regulated remain elusive. Here we employ vitro reconstitution with purified proteins to analyze role regulating microtubule binding. We find that autophosphorylates single residue within C-terminal tail restrict prevent aberrant hyperphosphorylation...
We generated a chimeric protein that comprises the receptor binding domain (RBD) from Spike (S) and nucleocapsid (N) antigens (SpiN) SARS-CoV-2 . Both vaccinated COVID-19 convalescent individuals presented memory CD4+ T CD8+ cell responses to SpiN. In rodents, SpiN elicited strong IFN -γ response by cells high levels of antibodies inactivated virus, but no neutralizing (nAbs). Furthermore, immunization Syrian hamsters human Angiotensin Convertase Enzyme-2-transgenic (K18-ACE-2) mice with...
Abstract We have systematically shown that the Amastigote Surface Protein-2 (ASP-2) and Transialidase (TS) antigens either in form of recombinant protein or encoded plasmids human adenovirus 5 (hAd5) confer strong protection against different lineages Trypanosoma cruzi parasites. Herein we generated a chimeric containing most immunogenic regions for T B cells from ASP-2 TS (DTT-1) evaluated its efficacy comparison with our standard protocol heterologous prime-boost using hAd5. Our results...