A5066482836- Research on Leishmaniasis Studies
- Trypanosoma species research and implications
- SARS-CoV-2 and COVID-19 Research
- Malaria Research and Control
- Parasites and Host Interactions
- Immunotherapy and Immune Responses
- Mosquito-borne diseases and control
- COVID-19 Clinical Research Studies
- Parasite Biology and Host Interactions
- vaccines and immunoinformatics approaches
- Synthesis and Biological Evaluation
- Vector-Borne Animal Diseases
- Leptospirosis research and findings
- Adenosine and Purinergic Signaling
- Immune cells in cancer
- Immune Cell Function and Interaction
- Toxoplasma gondii Research Studies
- T-cell and B-cell Immunology
- Hippo pathway signaling and YAP/TAZ
- Cytomegalovirus and herpesvirus research
- Diabetes and associated disorders
- Tryptophan and brain disorders
- T-cell and Retrovirus Studies
- IL-33, ST2, and ILC Pathways
- Influenza Virus Research Studies
Fundação Oswaldo Cruz
2021-2025
Universidade Federal de Minas Gerais
2011-2025
Pericytes are defined by both their anatomical location and molecular markers. Numerous publications have reported role as stem cells, contributing to the formation of tissues other than blood vessels. However, using cell-lineage tracing in a new transgenic mouse model, recent study shows that context aging some pathologies, Tbx18+ pericytes do not function cells vivo. This challenges current view can differentiate into reopen questions about plasticity. emerging knowledge is important only...
Background We evaluated kDNA PCR/hybridization and quantitative real-time PCR (qPCR) targeting the gene of DNA polymerase Leishmania infantum for CVL diagnosis assessment parasite load in clinical samples obtained invasively non-invasively. Methodology/Principal Findings Eighty naturally infected dogs from an endemic urban area Brazil were used. Animals divided into two groups based on presence or absence sings. Skin biopsies, bone marrow, blood conjunctival swabs collected submitted to L....
Abstract Both T cells and B have been shown to be generated after infection with SARS-CoV-2 yet protocols or experimental models study one the other are less common. Here, we generate a chimeric protein (SpiN) that comprises receptor binding domain (RBD) from Spike (S) nucleocapsid (N) antigens SARS-CoV-2. Memory CD4 + CD8 specific for SpiN could detected in blood of both individuals vaccinated Coronavac vaccine COVID-19 convalescent donors. In mice, elicited strong IFN-γ response by high...
Background The aim of this study was to evaluate the potential use nasal, oral, and ear swabs for molecular diagnosis canine visceral leishmaniasis (CVL) in an endemic urban area Brazil. Methodology/Principal Findings Sixty-two naturally infected ten healthy dogs were enrolled study. Bone marrow aspirates, peripheral blood, skin biopsy, conjunctival, collected. All samples, except submitted conventional PCR (cPCR) quantitative real time (qPCR) detect quantify Leishmania infantum DNA,...
ABSTRACT Toxoplasma gondii is a highly versatile parasite that infects most warm‐blooded animals and major cause of retinochoroiditis uveitis in humans. The pathophysiology these conditions remains poorly understood. Both virulence host inflammatory response contribute to the development ocular disease. While CD4 + T cells play critical role resistance infection, their kinetics effector functions, as well contribution clinical outcome including involvement, remain To address this question,...
The adaptive immunity against Plasmodium vivax is thought to be essential limit parasite growth during asymptomatic malaria, preventing the occurrence of symptoms. However, mechanisms governing clinical infections are not understood. Here, we investigated cellular compartment in P. vivax-infected individuals (ASY) compared symptomatic patients (SY) and healthy donors (CTL). Our integrative analysis revealed a TH1-biased immune signature with expanded populations TH1 CD4+ T cells associated...
Chagas disease (CD), caused by the parasite Trypanosoma cruzi, affects millions globally. Despite treatment options in acute phase, most infections progress to a chronic indeterminate form or develop severe cardiac/gastrointestinal complications. Understanding immune response is crucial for development of vaccines and more efficient drugs control. This work investigates T. cruzi H1 K68 strain infection female BALB/c C57BL/6 mice characterize differences Tfh B cell responses that may be...
Leishmania nested PCR (LnPCR) targeted to the SSUrRNA gene and DNA sequencing were used analyze 315 tissue samples from 80 Rattus norvegicus specimens trapped in an area endemic for leishmaniasis Belo Horizonte, Minas Gerais, Brazil. Of analyzed, 17.46% (55/315) of all tissues, 10% (8/80) skin, 26.92% (21/78) blood, 30.76% (24/78) bone marrow 2.53% (2/79) spleen positive Leishmania. The overall infection prevalence was 36.25% (29/80) showed that 65.51% (19/29) animals infected by parasites...
The need for daily parenteral administration is an important limitation in the clinical use of pentavalent antimonial drugs against leishmaniasis. In this study, amphiphilic antimony(V) complexes were prepared from alkylmethylglucamides (L8 and L10, with carbon chain lengths 8 10, respectively), their potential oral treatment visceral leishmaniasis (VL) was evaluated. Complexes Sb ligand at 1:3 (SbL8 SbL10) obtained reaction L8 as evidenced by elemental electrospray ionization-tandem mass...
Individuals with asymptomatic infection due to Plasmodium vivax are posited be important reservoirs of malaria transmission in endemic regions. Here we studied a cohort P . patients suburban area the Brazilian Amazon. Overall 1,120 individuals were screened for and 108 (9.6%) had parasitemia detected by qPCR but not microscopy. Asymptomatic higher levels antibodies against similar hematological biochemical parameters compared uninfected controls. Blood from very low transmitted main local...
ABSTRACT. The 1990s saw the rapid reemergence of malaria in Amazonia, where it remains an important public health priority South America. Amazonian International Center Excellence Malaria Research (ICEMR) was designed to take a multidisciplinary approach toward identifying novel control and elimination strategies. Based on geographically epidemiologically distinct sites Northeastern Peruvian Western Brazilian Amazon regions, synergistic projects integrate epidemiology, vector biology,...
Abstract We investigated possible vaccinia virus (VACV) in urban house cats Brazil. Serum samples from 6 were positive for VACV by PCR, indicating likely circulation among areas of This finding highlights the importance epidemiologic surveillance to avoid outbreaks human populations.
Abstract The present study aimed at evaluating the YF-specific neutralizing antibody profile besides a multiparametric analysis of phenotypic/functional features cell-mediated response elicited by 1/5 fractional dose 17DD-YF vaccine, administered as single subcutaneous injection. immunological parameters each volunteer was monitored two time points, referred as: before (Day 0) [Non-Vaccinated, NV (D0) ] and after vaccination 30–45) [Primary Vaccinees, PV (D30–45) ]. Data demonstrated high...
Abstract Chagas disease is a neglected tropical in Latin America and an imported emerging worldwide. Chronic cardiomyopathy (CCC) the most prominent clinical form can lead to heart failure, thromboembolism, sudden death. While previous reports have supported role for CD4 + T lymphocytes pathogenesis of CCC comprehensive analysis these cells during different forms lacking. Here, we used high-dimensional flow cytometry assess diversity circulating patients with distinct forms. We found...
Despite the very low or absent parasitism in lungs, interstitial pneumonitis is a common lesion found humans and dogs with visceral leishmaniasis. The lung neglected organ study of leishmaniasis, but represents an important characterized by thickening alveolar septum due to fibrosis inflammatory exudate, its pathogenesis still uncertain. In this study, polymerase chain reaction (PCR) was used detect Leishmania infantum paraffin-embedded biopsies from naturally infected endemic area Minas...
Dogs are the main reservoir of Leishmania infantum in endemic regions. Canine leishmaniasis, caused by L. , can progress to a chronic disease resulting death. Vaccines have been developed with certain degree success. The pathogenesis this is not completely understood, especially previously vaccinated dogs. We herein described clinical data, parasite load, serum levels cytokines, and potential vdogs fucose‐mannose ligand (FML)/QuilA saponin vaccine (Leishmune™) naturally infected (Vi)...
The present study aimed to evaluate the kinetics of phenotypic profile and integrative networks T/B-cells in severe COVID-19 patients, categorized according disease outcome, during circulation B.1.1.28 B.1.1.33 SARS-CoV-2 strains Brazil. Peripheral blood obtained at distinct time points (baseline/D0; D7; D14-28) was used for ex vivo flow cytometry immunophenotyping. data demonstrated a decrease D0 frequency CD3
BACKGROUND Eosinophils are granulocytes that rapidly increase frequency in the bloodstream during helminthic infections and allergic responses. They found tissue infected by Leishmania early disease, but their role infection is not entirely understood. OBJECTIVES We aim to compare disease due amazonensis BALB/c Δdbl-GATA1 mice, which lack eosinophils. METHODS mice with L. were observed for several weeks. The parasite load dissemination pattern assessed. FINDINGS developed an anticipated of a...
Chagas disease is a neglected parasitic caused by