A5089198656- Research on Leishmaniasis Studies
- Trypanosoma species research and implications
- SARS-CoV-2 and COVID-19 Research
- Animal Genetics and Reproduction
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- MicroRNA in disease regulation
- COVID-19 Clinical Research Studies
- Biotechnology and Related Fields
- SARS-CoV-2 detection and testing
- Immunotherapy and Immune Responses
- Cancer-related gene regulation
- Sperm and Testicular Function
- Biochemical and Structural Characterization
- Antimicrobial Peptides and Activities
- Virology and Viral Diseases
- Asthma and respiratory diseases
- Insect Resistance and Genetics
- CRISPR and Genetic Engineering
- Reproductive Biology and Fertility
- Chromosomal and Genetic Variations
- Aquaculture disease management and microbiota
- vaccines and immunoinformatics approaches
- Leptospirosis research and findings
- Influenza Virus Research Studies
- Biochemical and Molecular Research
Universidade de São Paulo
2019-2025
The Lundquist Institute
2025
Los Angeles Medical Center
2025
Harbor–UCLA Medical Center
2023-2024
UCLA Medical Center
2023-2024
Universidade Federal de Minas Gerais
2013-2022
Fundação Faculdade de Medicina
2021
Weatherford College
2021
University of Massachusetts Chan Medical School
2021
Hospital Sírio-Libanês
2020
ABSTRACT The serodiagnosis of human tegumentary leishmaniasis (TL) presents some problems, such as the low level antileishmanial antibodies found in most patients, well cross-reactivity subjects infected by other trypanosomatids. In present study, an immunoproteomic approach was performed aimed at identification antigens total extracts stationary-phase promastigote and amastigote-like forms Leishmania ( Viannia ) braziliensis using sera from TL patients. With purpose reducing identified...
Abstract Environmentally induced epigenetic transgenerational inheritance of phenotypic variation and disease susceptibility requires the germ cell (sperm or egg) transmission integrated mechanisms involving DNA methylation, histone modifications, non-coding RNA (ncRNA) actions. Previous studies have demonstrated that exposure disease-specific differential methylation regions (DMRs) in sperm are observed ncRNA-mediated occurs. The current study was designed to determine if exposure-specific...
Background The present study analyzed whether or not the in vitro cultivation for long periods of time pre-isolated Leishmania amazonensis from lesions chronically infected BALB/c mice was able to interfere parasites' infectivity using vivo and experiments. In addition, proteins that presented a significant decrease increase their protein expression content were identified applying proteomic approach. Methodology/Principal Findings Parasites cultured 150 days. Aliquots collected on day 0...
Abstract Both T cells and B have been shown to be generated after infection with SARS-CoV-2 yet protocols or experimental models study one the other are less common. Here, we generate a chimeric protein (SpiN) that comprises receptor binding domain (RBD) from Spike (S) nucleocapsid (N) antigens SARS-CoV-2. Memory CD4 + CD8 specific for SpiN could detected in blood of both individuals vaccinated Coronavac vaccine COVID-19 convalescent donors. In mice, elicited strong IFN-γ response by high...
Introduction Evidence demonstrates that sensory neurons respond to pathogenic/allergic infiltration and mediate immune responses, forming an integral part of host defense becomes hypersensitized during allergy. Our objective was investigate how asthmatic induction alters the pulmonary neuroimmune transcriptome. We hypothesized would upregulate genes in vagal ganglia (nodose/jugular ganglia), which be associated with immunity, these clustered, primarily nodose neurons. Furthermore, lungs...
Trypanosoma cruzi, the etiological agent of Chagas' disease, affects 8 million people predominantly living in socioeconomic underdeveloped areas. T. cruzi trypomastigotes (Ty), classical infective stage, interact with extracellular matrix (ECM), an obligatory step before invasion almost all mammalian cells different tissues. Here we have characterized proteome and phosphoproteome upon interaction ECM (MTy) data are available via ProteomeXchange identifier PXD010970. Proteins involved...
Leishmaniasis is a worldwide public health problem caused by protozoan parasites of the genus Leishmania. Leishmania braziliensis most important species responsible for tegumentary leishmaniases in Brazil. An understanding molecular mechanisms underlying success this parasite urgently needed. in-depth study on modulation gene expression across life cycle stages L. covering coding and noncoding RNAs (ncRNAs) was missing presented herein. Analyses differentially expressed (DE) genes revealed...
In trypanosomatids, regulation of gene expression occurs mainly at the posttranscriptional level, and RNA-binding proteins (RBPs) are key players in determining fates transcripts. RBPs targets protein arginine methyltransferases (PRMTs), which posttranslationally regulate capacity other RBP interactions by transferring methyl groups to residues (R-methylation). Herein, we functionally characterized five predicted PRMTs Leishmania braziliensis knockout endogenous HA tagging using CRISPR/Cas9...
Abstract The emergence and global dissemination of Severe Acute Respiratory Syndrome virus 2 (SARS-CoV-2) variants concern (VOCs) have been described as the main factor driving Coronavirus Disease 2019 pandemic. In Brazil, Gamma variant dominated epidemiological scenario during first period 2021. Many Brazilian regions detected Delta after its description documented spread. To monitor introduction spread VOC Delta, we performed Polymerase Chain Reaction (PCR) genotyping genome sequencing in...
Leishmania parasites alternate between hosts, facing environmental changes that demand rapid gene expression adaptation. Lacking canonical RNA polymerase II promoters, transcription in these eukaryotes is polycistronic, with regulation occurring post-transcriptionally. Although non-coding RNAs (ncRNAs) have been identified transcriptomes, their functions remain unclear. Recognizing structure's importance, we performed a genome-wide alignment of L. braziliensis and related species,...
Genetic variants of SARS-CoV-2 have been emerging and circulating in many places across the world. Rapid detection these is essential since their dissemination can impact transmission rates, diagnostic procedures, disease severity, response to vaccines or patient management. Sanger sequencing has used as preferred approach for variant among human immunodeficiency measles virus genotypes. Using primers amplify a fragment genome encoding part Spike protein, we showed that allowed us rapidly...
Leishmania major is the main causative agent of cutaneous leishmaniasis in Old World. In parasites, lack transcriptional control mostly compensated by post-transcriptional mechanisms. Methylation arginine a conserved post-translational modification executed Protein Arginine Methyltransferase (PRMTs). The genome from L . encodes five PRMT homologs, including cytosolic protein associated with several RNA-binding proteins, LmjPRMT7 It has been previously reported that could impact parasite...
Abstract Some varieties of eggplant are little susceptible to some plant pathogens, and antimicrobial peptides (AMPs) could be part this innate‐defence mechanism. This work aimed evaluate the in vitro activity peptide‐enriched fractions from cell wall extracts (CWE) soluble (SE) young plants fully expanded leaves eggplants against Ralstonia solanacearum Clavibacter michiganensis ssp. growth. The results indicated that highest inhibition level multiplication both bacteria was obtained with...
Adhesion of T. cruzi trypomastigotes to components the extracellular matrix (ECM) is an important step in mammalian host cell invasion. We have recently described a significant increase tyrosine nitration levels histones H2A and H4 when are incubated with ECM. In this work, we used chromatin immunoprecipitation (ChIP) anti-nitrotyrosine antibody followed by mass spectrometry identify nitrated DNA binding proteins detect alterations induced upon parasite incubation Histone H1, H2B, H3 were...
Despite rapid evolution across eutherian mammals, the X-linked MIR-506 family miRNAs are located in a region flanked by two highly conserved protein-coding genes (SLITRK2 and FMR1) on X chromosome. Intriguingly, these predominantly expressed testis, suggesting potential role spermatogenesis male fertility. Here, we report that were derived from MER91C DNA transposons. Selective inactivation of individual or clusters caused no discernible defects, but simultaneous ablation five containing 19...
Despite rapid evolution across eutherian mammals, the X-linked miR-506 family miRNAs are located in a region flanked by two highly conserved protein-coding genes ( Slitrk2 and Fmr1 ) on X chromosome. Intriguingly, these predominantly expressed testis, suggesting potential role spermatogenesis male fertility. Here, we report that were derived from MER91C DNA transposons their sequences diverged via LINE1-mediated retrotransposition during evolution. Selective inactivation of individual or...
Despite rapid evolution across eutherian mammals, the X-linked miR-506 family miRNAs are located in a region flanked by two highly conserved protein-coding genes ( Slitrk2 and Fmr1 ) on X chromosome. Intriguingly, these predominantly expressed testis, suggesting potential role spermatogenesis male fertility. Here, we report that were derived from MER91C DNA transposons. Selective inactivation of individual or clusters caused no discernable defects, but simultaneous ablation five containing...
Despite rapid evolution across eutherian mammals, the X-linked MIR-506 family miRNAs are located in a region flanked by two highly conserved protein-coding genes ( SLITRK2 and FMR1 ) on X chromosome. Intriguingly, these predominantly expressed testis, suggesting potential role spermatogenesis male fertility. Here, we report that were derived from MER91C DNA transposons. Selective inactivation of individual or clusters caused no discernible defects, but simultaneous ablation five containing...
Abstract Despite rapid evolution across eutherian mammals, the X-linked miR-506 family miRNAs are located in a region flanked by two highly conserved protein-coding genes ( Slitrk2 and Fmr1 ) on X chromosome. Intriguingly, these predominantly expressed testis, suggesting potential role spermatogenesis male fertility. Here, we report that were derived from MER91C DNA transposons. Selective inactivation of individual or clusters caused no discernable defects, but simultaneous ablation five...