A5066237562- Venomous Animal Envenomation and Studies
- Antimicrobial Peptides and Activities
- Healthcare and Venom Research
- Ion channel regulation and function
- Rabies epidemiology and control
- Insect and Pesticide Research
- Biochemical and Structural Characterization
- Nicotinic Acetylcholine Receptors Study
- Amphibian and Reptile Biology
- Chemical Synthesis and Analysis
- X-ray Diffraction in Crystallography
- Crystallization and Solubility Studies
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Sulfur-Based Synthesis Techniques
- Carbohydrate Chemistry and Synthesis
- Research on Leishmaniasis Studies
- Neurobiology and Insect Physiology Research
- Protein Hydrolysis and Bioactive Peptides
- Echinoderm biology and ecology
- Click Chemistry and Applications
- Marine Invertebrate Physiology and Ecology
- Catalytic Cross-Coupling Reactions
- Marine Sponges and Natural Products
- Lipid Membrane Structure and Behavior
- Protease and Inhibitor Mechanisms
Instituto Butantan
2016-2025
Universidade Estadual Paulista (Unesp)
2010-2024
Centro de Computação Gráfica
2023
Universidade São Francisco
2020
University of St. Francis
2020
Universidade Cidade de São Paulo
2020
Instituto de Pesquisas Energéticas e Nucleares
2020
National University of the Northeast
2020
Centro de Excelência em Bioinformática
2020
Fundação Oswaldo Cruz
2020
Peroxiredoxins (Prx) are widely distributed peroxidases that can be divided into 1-Cys and 2-Cys Prx groups based on the number of conserved cysteine residues participate in their catalytical cycle. have been described to strictly dependent thiols, but here, we show ascorbate (vitamin C) also reduces Prx, not from several taxonomic groups. Reduction by is partly related fact oxidized form a stable sulfenic acid (Cys-SOH) instead disulfide. In addition, histidine residue active site required....
We investigated the putative toxins of Philodryas olfersii (Colubridae), a representative family snakes neglected in venom studies despite their growing medical importance. Transcriptomic data gland complemented by proteomic analysis secretion revealed presence major toxin classes from Viperidae family, including serine proteases, metalloproteases, C‐type lectins, Crisps, and natriuretic peptide (CNP). Interestingly, phylogenetic CNP precursor showed it as linker between two related...
Apis mellifera venom is comprised basically of melittin, phospholipase A(2), histamine, hyaluronidase, catecholamine and serotonin. Some these components have been associated with allergic reactions, amongst several other symptoms. On the hand, bee mass stinging, caused by Africanized honey (AHB), increasingly becoming a serious public health issue in Brazil; therefore, development efficient serum-therapies has become necessary. In this work, we analyzed composition AHB Brazil through one...
ABSTRACT The serodiagnosis of human tegumentary leishmaniasis (TL) presents some problems, such as the low level antileishmanial antibodies found in most patients, well cross-reactivity subjects infected by other trypanosomatids. In present study, an immunoproteomic approach was performed aimed at identification antigens total extracts stationary-phase promastigote and amastigote-like forms Leishmania ( Viannia ) braziliensis using sera from TL patients. With purpose reducing identified...
Among vertebrates, the yolk is commonly only form of nutritional investment offered by female to embryo. Some species, however, have developed parental care behaviors associated with specialized food provisioning essential for offspring survival, such as production lipidic-rich milk in mammals. Here, we show that females egg-laying caecilian amphibian
Africanized bees have spread across the Americas since 1956 and consequently resulted in human animal deaths attributed to massive attacks related exposure from Argentina USA. In Brazil, more than 100,000 accidents were registered last 5 years with a total of 303 deaths. To treat such attacks, Brazilian researchers developed first specific antivenom against honey bee sting exposure. This unique product, its kind world, has been safely tested 20 patients during Phase 2 clinical trial. develop...
Sea anemones are an important source of various biologically active peptides, and it is known that ATX-II from Anemonia sulcata slows sodium current inactivation. Using six different channel genes (from Nav1.1 to Nav1.6), we investigated the differential selectivity toxins AFT-II (purified Anthopleura fuscoviridis) Bc-III Bunodosoma caissarum) compared their effects with those recorded in presence ATX-II. Interestingly, differ by only one amino acid (L36A) has 70% similarity. The three...
Abstract Variation in the snake venom proteome is well documented and it a ubiquitous phenomenon at all taxonomical levels. However, variation peptidome so far not described. In this work we used mass spectrometry [liquid chromatography/mass (LC/MS) matrix‐assisted laser desorption/ionization time‐of‐flight (MALDI‐TOFMS)] to explore sex‐based differences among peptides of eighteen sibling specimens Bothrops jararaca single litter born raised laboratory. MALDI‐TOFMS analyses showed individual...
Amphibians are known by cutaneous glands, spread over the skin, containing toxins (proteins, peptides, biogenic amines, steroidal bufadienolides, and alkaloids) used as chemical defense against predators microbial infection. Toads characterized presence of parotoid macroglands. The common toads have lately been divided into two genera: Bufo (Europe, Asia, Africa) Rhinella (South America). Basal Rhaebo genus is exclusively Central America Amazon region. Although related, species may share...
Amphibians have many skin poison glands used in passive defense, which the aggressor causes its own poisoning when biting prey. In some amphibians accumulate certain regions forming macroglands, such as parotoids of toads. We discovered that toad Rhaebo guttatus is able to squirt jets towards aggressor, contradicting typical amphibian defense. studied R. chemical comparing it with Rhinella marina, a sympatric species showing found only parotoid adhered scapula and do not calcified dermal...
Apis mellifera venom, which has already been recommended as an alternative anti-inflammatory treatment, may be also considered important source of candidate molecules for biotechnological and biomedical uses, such the treatment parasitic diseases.Africanized honeybee venom from was fractionated by RP-C18-HPLC obtained melittin incubated with promastigotes intracellular amastigotes Leishmania (L.) infantum. Cytotoxicity to mice peritoneal macrophages evaluated through mitochondrial oxidative...
Palladium(0)-catalyzed cross-coupling between potassium aryltrifluoroborate salts and aryl tellurides proceeds readily to afford the desired biaryls in good excellent yield. The reaction seems be unaffected by presence of electron-withdrawing or electron-donating substituents both partners. Biaryls containing a variety functional groups can prepared. A chemoselectivity study was also carried out using bearing halogen atoms same compound. In addition, this new version Suzuki−Miyaura monitored...
Abstract In amphibians, secretions of toxins from specialized skin poison glands play a central role in defense against predators. The production toxic is often associated with conspicuous color patterns that warn potential predators, as it the case many dendrobatid frogs, including Ameerega picta . This species resembles presumably nontoxic Leptodactylus lineatus study tests for mimicry by studying morphology and distribution glands, components secretion, defensive behavior. Dorsal was...
Enolase is secreted by C. albicans and present in its biofilms although extracellular function unknown. Here we show that enolase mediates the colonization of small intestine mucosa albicans. Assays using intestinal disks adhesion inhibited, a dose dependent mode, either pretreatment epithelium with recombinant (70% at 0.5 mg/ml enolase) or yeasts anti-enolase antibodies (48% 20 µg antiserum). Also flow cytometry, immunoblots conditioned media confocal microscopy demonstrate not an artifact...
Rabies is a fatal zoonotic neglected disease that occurs in more than 150 countries, and kills 55.000 people every year. It caused by an enveloped single stranded RNA virus affects the central nervous system, through infection initiated muscular nicotinic acetylcholine receptor, according to many authors. Alkaloids, such as acetylcholine, are widespread molecules nature. They present numerous biological fluids, including skin secretion of amphibians, which they act (together with proteins,...
Abstract Spiders have distinct predatory behaviours selected along Araneae’s evolutionary history but are mainly based on the use of venom for prey paralysis. Uloboridae spiders lost their glands secondarily during evolution. Because this, they immobilise by extensively wrapping, and digestion starts with addition digestive fluid. During extra-oral digestion, fluid liquefies both AcSp2 spidroins from web fibres. Despite efficiency this process, cocktail enzymes involved in remains unknown....