A5028222469- Insect Resistance and Genetics
- Insect Utilization and Effects
- Neurobiology and Insect Physiology Research
- Venomous Animal Envenomation and Studies
- Silkworms and Sericulture Research
- Enzyme Production and Characterization
- Invertebrate Immune Response Mechanisms
- Insect Pest Control Strategies
- Insect and Pesticide Research
- Antimicrobial Peptides and Activities
- Biofuel production and bioconversion
- Studies on Chitinases and Chitosanases
- Aquaculture Nutrition and Growth
- Insect-Plant Interactions and Control
- Research on Leishmaniasis Studies
- Protein Hydrolysis and Bioactive Peptides
- Physiological and biochemical adaptations
- Spider Taxonomy and Behavior Studies
- Ion channel regulation and function
- Biochemical Acid Research Studies
- Biochemical and Structural Characterization
- Protease and Inhibitor Mechanisms
- Aldose Reductase and Taurine
- Sugarcane Cultivation and Processing
- Breastfeeding Practices and Influences
Instituto Butantan
2011-2024
Universidade do Porto
2023-2024
Universidade Federal do Pará
2024
Universidade de São Paulo
2001-2023
Governo do Distrito Federal
2022
National School of Political Science and Public Administration
2022
Universidade de Pernambuco
2021
Universidade Federal do Amazonas
2015
Biotech (Brazil)
2015
Abstract Many plant proteinase inhibitors have lysine at the P1 position, presumably to avoid hydrolysis by insect trypsins. Lepidopteran trypsins appear adapted resist through increased inhibitor and decreased binding hydrophilic reactive sites. digestive prefer position substrate subsites more hydrophobic than from insects in other orders. All available sequences of sensitive inhibitor‐insensitive were aligned with porcine trypsin, for which interactions Kunitz Bowman‐Birk are known. After...
The development of transgenic maize plants expressing soybean proteinase inhibitors could reduce the economic damage one major pests in Brazil, fall armyworm, Spodoptera frugiperda (J.E. Smith, 1797). We examined influence on digestive enzyme properties and S. larvae. inhibition trypsin chymotrypsin activities vitro by suggested that either Kunitz (SBTI) or Bowman-Birk (SBBI) would have a potential antimetabolic effect when ingested insect However, chronic ingestion semipurified did not...
Abstract One approach that can be employed in integrated pest management is the use of proteins with antinutritional effects on insect metabolism and development. The antimetabolic properties soybean proteinase inhibitor (SPI) growth neonate larvae sugarcane borer, Diatraea saccharalis (Fabricius, 1794) (Lepidoptera: Crambidae) have been evaluated. When incorporated into an artificial diet at 0.5% (w/w), SPI retarded rate development when compared fed alone. However, larval survival was not...
Scorpions are among the oldest terrestrial arthropods and they have passed through small morphological changes during their evolutionary history on land. They efficient predators capable of capturing consuming large preys due to envenomation these animals can become a human health challenge. Understanding physiology scorpions not only lead insights but also is crucial step in development control strategies. However, digestive process has been scarcely studied. In this work, we describe...
Abstract Spiders have distinct predatory behaviours selected along Araneae’s evolutionary history but are mainly based on the use of venom for prey paralysis. Uloboridae spiders lost their glands secondarily during evolution. Because this, they immobilise by extensively wrapping, and digestion starts with addition digestive fluid. During extra-oral digestion, fluid liquefies both AcSp2 spidroins from web fibres. Despite efficiency this process, cocktail enzymes involved in remains unknown....
Spiders are known for their predatory efficiency and high capacity of digesting relatively large prey. They do this by combining both extracorporeal intracellular digestion. Whereas many throughput ("-omics") techniques focus on biomolecules in spider venom, so far approach has not yet been applied to investigate the protein composition midgut diverticula (MD) digestive fluid (DF). We here report our investigations MD DF Nephilingis (Nephilengys) cruentata through use next generation...
O Brasil enfrenta sérios desafios em relação à insegurança alimentar, especialmente entre a população negra situação de vulnerabilidade social e econômica. Entre os programas complementares saúde, destacam-se as hortas comunitárias, que têm se mostrado uma ferramenta importante para garantir o acesso alimentação saudável promover inclusão social. As comunitárias são espaços colaborativos onde alimentos frescos, como vegetais frutas, cultivados pela própria comunidade, contribuindo melhorar...
Abstract Insect digestive chymotrypsins are present in a large variety of insect orders but their substrate specificity still remains unclear. Four from 3 different (Dictyoptera, Coleoptera, and two Lepidoptera) were isolated using affinity chromatography. Enzymes presented molecular masses the range 20 to 31 kDa pH optima 7.5 10.0. Kinetic characterization colorimetric fluorescent substrates indicated that differ bovine chymotrypsin primary toward small (like N‐ benzoyl‐L‐Tyr p‐...
In plants, it is well-known that ascorbic acid (vitamin C) can be synthesized via multiple metabolic pathways but there still much to learned concerning their integration and control mechanisms. Furthermore, the structural biology of component enzymes has been poorly exploited. Here we describe first crystal structure for an L-galactose dehydrogenase [Spinacia oleracea GDH (SoGDH) from spinach], D-mannose/L-galactose (Smirnoff-Wheeler) pathway which converts into L-galactono-1,4-lactone. The...
A detecção do sexo de mosquitos da família Culicidae é importante em estudos faunísticos e epidemiológicos, pois somente as fêmeas possuem competência vetora para patógenos. O dimorfismo sexual genitália apêndices cefálicos é, geral, facilmente visível culicídeos. As asas também podem ser dimórficas assim poderiam complementar o procedimento sexagem. No entanto, tal distinção não notável à observação direta. Visando descrever formalmente alar Aedes scapularis, um culicídeo vetorialmente...
Anopheles aquasalis is a major malaria vector in coastal areas of South and Central America where it breeds preferentially brackish water. This species very susceptible to Plasmodium vivax has been already incriminated as responsible outbreaks. There no high-throughput investigation into the sequencing An. genes, transcripts proteins despite its epidemiological relevance. Here we describe sequencing, assembly annotation transcriptome.
Abstract Spiders are important predators of insects and their venoms play an essential role in prey capture. Spider have several potential applications as pharmaceutical compounds insecticides. However, transcriptomic proteomic analyses the digestive system (DS) spiders show that DS is also a rich source new peptidase inhibitor molecules. Biochemical, data crude extracts presence molecules with spider Nephilingis cruentata . Therefore, aims this work were to isolate characterize trypsin...
Abstract l ‐fucose is a constituent of glycoconjugates in different organisms. Fucosidases catalyze the removal fucose residues, and have been correlated to physiological pathological processes, such as fertilization, cancer, fucosidosis, digestion molluscs ticks. An α‐ ‐fucosidase sequence was identified from transcriptome proteome midgut diverticula synanthropic spider Nephilingis cruentata . In this article, we describe isolation characterization its activity using substrates inhibitors...
α-l-Fucosidases are widely occurring enzymes that remove fucose residues from N- and O-fucosylated glycoproteins. Comparison of amino acid sequences fucosidases reveals although the nucleophile is conserved among all α-l-fucosidases, position acid/base residue quite variable. Although several site-directed mutation studies have previously been performed on bacterial fucosidases, only eukaryotic fucosidase so studied was human fucosidase. Recent alignments indicate Arthropoda α-l-fucosidases...
Abstract Trehalose-6-phosphate (T6P) is an intermediate of trehalose biosynthesis that plays essential role in plant metabolism and development. Here, we comprehensively analyzed sequences from enzymes sugarcane, one the main crops used for bioenergy production. We identified protein domains, phylogeny, silico expression levels all classes enzymes. However, post-translational modifications residues involved catalysis substrate binding were only trehalose-6-phosphate synthase (TPS) sequences....