Márcio C. Silva-Filho

ORCID: 0000-0003-2129-0388
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About
Contact & Profiles
Research Areas
  • Insect Resistance and Genetics
  • Photosynthetic Processes and Mechanisms
  • Sugarcane Cultivation and Processing
  • Insect-Plant Interactions and Control
  • Biofuel production and bioconversion
  • Plant Molecular Biology Research
  • Insect Pest Control Strategies
  • Plant-Microbe Interactions and Immunity
  • Biochemical and Structural Characterization
  • Plant Virus Research Studies
  • Studies on Chitinases and Chitosanases
  • Plant Parasitism and Resistance
  • Plant Reproductive Biology
  • Plant Pathogenic Bacteria Studies
  • Mitochondrial Function and Pathology
  • Entomopathogenic Microorganisms in Pest Control
  • RNA and protein synthesis mechanisms
  • Plant Stress Responses and Tolerance
  • Plant nutrient uptake and metabolism
  • Genomics and Phylogenetic Studies
  • Enzyme Production and Characterization
  • Transgenic Plants and Applications
  • Plant tissue culture and regeneration
  • Insect symbiosis and bacterial influences
  • ATP Synthase and ATPases Research

Universidade de São Paulo
2014-2023

Universidade Católica Dom Bosco
2020-2021

Universidade Federal de Mato Grosso do Sul
2014

Planta
2014

Forest Science and Research Institute
2014

GTx (United States)
2012

Universidade Estadual de Campinas (UNICAMP)
2010

Altera (United States)
2002

Methodist University of Piracicaba
2001

UCLouvain
1994-1997

Global interest in sugarcane has increased significantly recent years due to its economic impact on sustainable energy production. Sugarcane breeding and better agronomic practices have contributed a huge increase yield the last 30 years. Additional increases are expected result from use of biotechnology tools near future. Genetically modified (GM) that incorporates genes resistance biotic abiotic stresses could play major role achieving this goal. However, bring GM market, it is necessary...

10.1007/s12042-011-9068-3 article EN cc-by-nc Tropical Plant Biology 2011-02-21

In this review, plant-insect interaction is discussed as a dynamic system, subjected to continual variation and change. Plants developed different mechanisms reduce insect attack, including specific responses that activate metabolic pathways which considerably alter their chemical physical aspects. On the other hand, insects several strategies overcome plant defense barriers, allowing them feed, grow reproduce on host plants. This review foccuses aspects of complex between plants insects,...

10.1590/s1677-04202002000200001 article EN Brazilian Journal of Plant Physiology 2002-08-01

Abstract Background Sugarcane is an increasingly economically and environmentally important C4 grass, used for the production of sugar bioethanol, a low-carbon emission fuel. originated from crosses Saccharum species noted its unique capacity to accumulate high amounts sucrose in stems. Environmental stresses limit enormously sugarcane productivity worldwide. To investigate transcriptome changes response environmental inputs that alter yield we cDNA microarrays profile expression 1,545 genes...

10.1186/1471-2164-8-71 article EN cc-by BMC Genomics 2007-03-13

The fall armyworm, Spodoptera frugiperda (J. E. Smith 1797) (Lepidoptera: Noctuidae), is the most important corn pest in South America. Larvae feed mostly on leaves, but also ears when population densities are high. This has been historically controlled with insecticide applications, many cases of resistance have limited their efficacy. Transgenic varieties expressing Bacillus thuringiensis proteins (Bt corn) a widely adopted alternative to insecticides and, past 8 yr, primary technology for...

10.1093/jipm/pmx011 article EN cc-by-nc-nd Journal of Integrated Pest Management 2017-01-01

Rapid alkalinization factor (RALF) is a peptide signal that plays basic role in cell biology and most likely regulates expansion. In this study, transgenic Arabidopsis thaliana lines with high low levels of AtRALF1 transcripts were used to investigate peptide’s mechanism action. Overexpression the root-specific isoform resulted reduced size. Conversely, silencing increased root length by increasing size cells. AtRALF1-silenced plants also showed an increase number lateral roots, whereas...

10.1093/jxb/eru099 article EN Journal of Experimental Botany 2014-03-11

Abstract Vector-borne plant pathogens often change host traits to manipulate vector behavior in a way that favors their spread. By contrast, infection by opportunistic fungi does not depend on vectors, although damage caused an herbivore may facilitate infection. Manipulation of hosts and such as insect herbivores, has been demonstrated interactions with fungal pathogens. Herein, we establish new paradigm for the plant-insect-fungus association sugarcane. It long assumed Fusarium...

10.1038/s41396-021-01010-z article EN cc-by The ISME Journal 2021-06-14

Proteins found in the root exudates are thought to play a role interactions between plants and soil organisms. To gain better understanding of protein secretion by roots, we conducted systematic proteomic analysis Arabidopsis thaliana at different plant developmental stages. In total, identified 111 proteins secreted majority which were exuded constitutively during all stages development. However, defense-related such as chitinases, glucanases, myrosinases, others showed enhanced flowering....

10.1074/jbc.m110.119040 article EN cc-by Journal of Biological Chemistry 2010-08-04

Abstract Sugarcane is a proven biofuel feedstock and accounts for about 40% of the production worldwide. It has more favorable energy input/output ratio than that corn, other major feedstock. The rich resource genetic diversity plasticity autopolyploid genomes offer wealth opportunities application genomics technologies to address fundamental questions in sugarcane towards maximizing biomass production. In workshop on engineering held at Rutgers University, we identified research areas...

10.1111/j.1757-1707.2009.01016.x article EN GCB Bioenergy 2009-06-01

Protein-protein interactions (PPIs) constitute one of the most crucial conditions to sustain life in living organisms. To study PPI Arabidopsis thaliana we have developed AtPIN, a database and web interface for searching building interaction networks based on publicly available protein-protein datasets. All were divided into experimentally demonstrated or predicted. The PPIs AtPIN present cellular compartment classification (C3) which divides 4 classes according its evidence subcellular...

10.1186/1471-2105-10-454 article EN cc-by BMC Bioinformatics 2009-12-01

Sugarcane is a highly productive crop used for centuries as the main source of sugar and recently to produce ethanol, renewable bio-fuel energy source. There increased interest in this due impending need decrease fossil fuel usage. has polyploid genome. Expressed sequence tag (EST) sequencing significantly contributed gene discovery expression studies associate function with sugarcane genes. A significant amount data exists on regulatory events controlling responses herbivory, drought,...

10.1155/2008/458732 article EN cc-by International Journal of Plant Genomics 2007-12-31

Sugarcane is one of the most important agricultural crops in world. However, pathogen infection and herbivore attack cause constant losses yield. Plants respond to by inducing expression several protein types, such as glucanases, chitinases, thaumatins, peptidase inhibitors, defensins, catalases glycoproteins. Proteins induced pathogenesis are directly or indirectly involved plant defense, leading death other defense responses. Several these proteins sugarcane different pathogens insects...

10.1590/1678-4685-gmb-2016-0057 article EN cc-by Genetics and Molecular Biology 2017-02-20

Prohormone proteins in animals and yeast are typically processed at dibasic sites by convertases. Propeptide hormones also found plants but little is known about processing. We show for the first time that a site upstream of plant peptide hormone, AtRALF1, essential Overexpression preproAtRALF1 causes semi‐dwarfism whereas overexpression preproAtRALF1(R69A), propeptide with mutation site, shows normal phenotype. RALF1(R69A) accumulate only mutated proprotein not peptide. In vitro processing...

10.1016/j.febslet.2008.08.025 article EN FEBS Letters 2008-09-05

The rapid alkalinization factor (RALF) peptide negatively regulates cell expansion, and an antagonistic relationship has been demonstrated between AtRALF1, a root-specific RALF isoform in Arabidopsis, brassinosteroids (BRs). An evaluation of the response BR signaling mutants to AtRALF1 revealed that BRI1-associated receptor kinase1 (bak1) are insensitive root growth inhibition activity. BAK1 was essential for induction AtRALF1-responsive genes but showed no effect on mobilization Ca2+...

10.1371/journal.pgen.1007053 article EN cc-by PLoS Genetics 2017-10-13

The development of transgenic maize plants expressing soybean proteinase inhibitors could reduce the economic damage one major pests in Brazil, fall armyworm, Spodoptera frugiperda (J.E. Smith, 1797). We examined influence on digestive enzyme properties and S. larvae. inhibition trypsin chymotrypsin activities vitro by suggested that either Kunitz (SBTI) or Bowman-Birk (SBBI) would have a potential antimetabolic effect when ingested insect However, chronic ingestion semipurified did not...

10.1603/0022-0493-93.3.892 article EN Journal of Economic Entomology 2000-06-01

Abstract One approach that can be employed in integrated pest management is the use of proteins with antinutritional effects on insect metabolism and development. The antimetabolic properties soybean proteinase inhibitor (SPI) growth neonate larvae sugarcane borer, Diatraea saccharalis (Fabricius, 1794) (Lepidoptera: Crambidae) have been evaluated. When incorporated into an artificial diet at 0.5% (w/w), SPI retarded rate development when compared fed alone. However, larval survival was not...

10.1046/j.1570-7458.2001.00804.x article EN Entomologia Experimentalis et Applicata 2001-04-01

Abstract The purpose of this research was to evaluate the genetic similarity and structure fall armyworm, Spodoptera frugiperda (J.E. Smith), populations associated with maize cotton crops in Brazil using amplified fragment length polymorphisms. Mean among 0.45. unweighted pair group method arithmetic mean analysis dendrograms did not separate S. into clusters related host plant which insects were collected. No variation observed , suggesting that same are injuring both Brazil. This...

10.1017/s0007485307004944 article EN Bulletin of Entomological Research 2007-05-24
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