A5043453536- Research on Leishmaniasis Studies
- Trypanosoma species research and implications
- Insect and Pesticide Research
- Toxin Mechanisms and Immunotoxins
- Brucella: diagnosis, epidemiology, treatment
- Viral Infections and Vectors
- T-cell and B-cell Immunology
- Parasites and Host Interactions
- Monoclonal and Polyclonal Antibodies Research
- Burkholderia infections and melioidosis
- Lysosomal Storage Disorders Research
- Vector-borne infectious diseases
- Renal Diseases and Glomerulopathies
- Leptospirosis research and findings
- Bacterial Identification and Susceptibility Testing
- Fungal Infections and Studies
- Vector-Borne Animal Diseases
- Muscle metabolism and nutrition
- Antibiotic Resistance in Bacteria
- Escherichia coli research studies
- Mosquito-borne diseases and control
- Antifungal resistance and susceptibility
- Systemic Lupus Erythematosus Research
- Hepatitis Viruses Studies and Epidemiology
- Clostridium difficile and Clostridium perfringens research
Universidade Federal de Minas Gerais
2016-2025
Hospital Israelita Albert Einstein
2024
Fundação Oswaldo Cruz
2006-2008
Inserm
2008
Universidade Federal do Piauí
2002-2007
Universidade Estadual do Piau
2006
The first proteomic analysis of Trypanosoma cruzi resistance to Benznidazole (BZ) is presented. differential proteome T. with selected in vivo (BZR and Clone27R), its susceptible pairs (BZS Clone9S), a pair from population Benznidazole-in vitro-induced (17LER) the 17WTS were analyzed by two-dimensional gel electrophoresis (2-DE) followed mass spectrometry (MS) for protein identification. Out 137 spots through MS, 110 identified as 56 distinct proteins. proteins, 36 present resistant, 9...
Background Visceral leishmaniasis is the most severe form of leishmaniasis. Worldwide, approximately 20% zoonotic human visceral caused by Leishmania infantum, also known as chagasi in Latin America. Current diagnostic methods are not accurate enough to identify Leishmania-infected animals and may compromise effectiveness disease control. Therefore, we aimed produce test two recombinant multiepitope proteins a means improve increase accuracy diagnosis canine (CVL). Methodology/Principal...
RESUMO A leishmaniose visceral e a mais severa dentre as leishmanioses sua forma zoonotica, causada por Leishmania (Leishmania) infantum chagasi, representa 20% do registro de casos da humana mundial. Nas areas urbanas periurbanas dos tropicos, incidencia dessa doenca crescente. Uma das medidas controle adotada no Brasil identificacao eliminacao caes infectados, considerados os principais reservatorios urbana. Nesse contexto, o diagnostico confiavel nesses animais desempenha um papel...
Dogs naturally infected with Leishmania Infantum (=L. chagasi) were treated miltefosine using different therapeutic regimens. The animals evaluated for clinical evolution, biochemical parameters, parasite load (by real-time PCR), cytokine levels and humoral response. After treatment during the following 24 months, there was progressive improvement complete recovery in 50% (7/14) of animals. There a decrease smear positivity bone marrow after treatment, also gradual constant positive cultures...
Abstract Background We compared skin biopsy samples from different anatomical regions for detecting Leishmania in dogs, using histological (HE), immunohistochemical (IHC) and polymerase chain reaction (PCR) techniques. Results The sensitivity was 82.8 percent PCR, 62.1 IHC 44.8 HE. These methods do not appear to depend on the clinical status of animal or source sample; there is no "best region" any method. However, PCR more effective than HE ear nose whereas better samples. There weak...
Identification of novel antigens is essential for developing new diagnostic tests and vaccines. We used DIGE to compare protein expression in amastigote promastigote forms Leishmania chagasi. Nine hundred spots were visualized. Five amastigote-specific, 25 promastigote-specific, 10 proteins shared by the two parasite stages identified. Furthermore, 41 identified Western blot employing 2-DE sera from infected dogs. From these proteins, 3 38 reactive with IgM total IgG, respectively. The...
Background Visceral leishmaniasis is the most severe form of leishmaniasis. Approximately 20% zoonotic human visceral worldwide caused by Leishmania infantum, which also known as chagasi in Latin America, and disease incidence increasing urban peri-urban areas tropics. In this disease, dogs are main reservoirs. Diagnostic methods used to identify infected animals not able detect all ones, can compromise effectiveness control. Therefore, contribute improvement diagnostic for canine (CVL), we...
Knowledge of Leishmania virulence is essential for understanding how the contact between pathogen and host cells can lead to pathogenesis. Virulence in two L. infantum strains was characterized using macrophages hamsters. Next, we used difference gel electrophoresis (DIGE) mass spectrometry identify differentially expressed proteins. A total 63 spots were identified corresponding 36 proteins; 20 up-regulated, which 16 had been previously associated with virulence. Considering our results...
This work presents the first indirect immunoassay performed on a paper-based microfluidic platform for diagnosis of canine visceral leishmaniasis (CVL). The IgG antibody biomarker, which signifies presence this infectious disease, was captured with recombinant K39 antigen and detected secondary antibodies that were conjugated cleavable ionic probes. use probes enabled direct analysis assay results through an on-chip paper spray mass spectrometry (MS) technology. MS-based developed to allow...
Leprosy is a chronic infectious disease that continues to be present in more than 120 countries. In 2023, over 20,000 new cases were reported Brazil, making it the second most endemic country world. The development of information systems utilize available clinical records essential support decision-making during treatment this highly stigmatizing disease. study, we preliminary results demonstrating feasibility using YOLO for automatic recognition non-textual data from neurological...
Angiotensin-(1-7) has been described as a new potential therapeutic tool for the treatment and prevention of metabolic disorders by regulating several pathways in visceral white adipose tissue (vWAT). The aim this study was to access proteins differentially regulated Ang-(1-7) using proteomic analysis tissue. Male mice were divided into three groups fed 60 days, with each group receiving one following diets: standard diet+HPβCD (ST), high fat (HFD) diet+Ang-(1-7)/HPβCD (HFD+Ang-(1-7)). Body...
Herein, we intended to perform flow-cytometric analyses of peripheral blood NK-cell subsets in patients with active tuberculosis (TB) and those putative resistant subjects displaying positive tuberculin skin test (TST+) compared TST- healthy controls. Our findings demonstrated distinct phenotypic features TST+ as TB. While lower values NK-cells increased frequency CD3-CD16+ CD56- CD3-CD16-CD56+ besides CD56+ was observed TST+, unaltered levels found Additional analysis highlighted a shift...
Aim: To identify immunoreactive proteins of Cryptococcus gattii genotype VGII and their B-cell epitopes. Materials & methods: We combined 2D gel electrophoresis, immunoblotting mass spectrometry to from four strains C. (CG01, CG02, CG03 R265). Next, we screened the identified map Results: Sixty-eight were identified. The number found were: CG01 (12), CG02 (18) R265 (26). In addition, mapped 374 peptides potentially targeted by B cells. Conclusion: Both epitopes that a host humoral response...
This study involves the comparison between exoproteomes of two different strains Corynebacterium pseudotuberculosis, etiologic agent caseous lymphadenitis in small ruminants. In a previous study, based on gel-free system (TPP-LC/MS(E)), 70 exoproteins for strain 1002 and 67 C231, totaling 93 extracellular proteins C. were identified. present work, we have used 2D gel electrophoresis to resolve both strains, which then digested with trypsin, analyzed by MALDI-TOF/TOF identified software...