The protozoan parasite Trypanosoma cruzi is the etiologic agent of Chagas disease, a highly debilitating human pathology that affects millions people in Americas. sequencing this parasite's genome reveals trans-sialidase/trans-sialidase-like (TcS), polymorphic protein family known to be involved several aspects T. biology, largest gene family, encoding more than 1,400 genes. Despite fact four TcS groups are well characterized and only one contains active trans-sialidases, all members...

Background Trypanosoma cruzi is the etiological agent of Chagas disease, a debilitating illness that affects millions people in Americas. A major finding T. genome project was discovery novel multigene family composed approximately 1,300 genes encode mucin-associated surface proteins (MASPs). The high level polymorphism MASP associated with its localization at infective forms parasite suggests participates host–parasite interactions. We speculate large repertoire sequences may contribute to...

Abstract Background Although the pancreatic ductal adenocarcinoma (PDAC) presents high mortality and metastatic potential, there is a lack of effective therapies low survival rate for this disease. This PDAC scenario urges new strategies diagnosis, drug targets, treatment. Methods We performed gene expression microarray meta-analysis tumor against normal tissues in order to identify differentially expressed genes (DEG) shared among all datasets, named core-genes (CG). confirmed CG protein...

Identification of novel antigens is essential for developing new diagnostic tests and vaccines. We used DIGE to compare protein expression in amastigote promastigote forms Leishmania chagasi. Nine hundred spots were visualized. Five amastigote-specific, 25 promastigote-specific, 10 proteins shared by the two parasite stages identified. Furthermore, 41 identified Western blot employing 2-DE sera from infected dogs. From these proteins, 3 38 reactive with IgM total IgG, respectively. The...

Infection by Leishmania protozoan parasites can, depending on the parasite species, cause a variety of disease outcomes in humans and other mammals, from single self-healing cutaneous lesions to visceral dissemination parasite. The correlation between chronic ecto-nucleotidase activity surface is addressed here using damage caused epithelial cells nitric oxide. In order explore role purinergic metabolism lesion formation outcome infection, we implemented cellular automata/lattice gas model...

Comparative genomic analysis of strains may help us to better understand the wide diversity their genetic profiles. The aim this study was analyse features resistome and virulome Brazilian first methicillin-resistant Staphylococcus aureus (MRSA) isolates relationship other international MRSA strains.The whole genomes three previously isolated in Vitória da Conquista were sequenced, assembled, annotated compared with genomes. A phylogenetic tree constructed pan-genome accessory core...

Leishmania spp. commonly infects phagocytic cells of the immune system, particularly macrophages, employing various evasion strategies that enable their survival by altering intracellular environment. In mammals, these parasites establish persistent infections modulating gene expression in macrophages thus interfering with signaling and response pathways ultimately creating a favorable environment for parasite reproduction. this study, our objective was to use data mining subsequent...

Chagas disease, caused by the Trypanosoma cruzi, has a wide distribution in South America, and its main method of control is elimination triatomines. It presented here geographic rate natural infection T. cruzi triatomines collected evaluated from 2008 to 2013 southwest Bahia. Triatomines were captured intradomiciliary peridomiciliary areas five cities located Bahia state, identified, analyzed for presence trypanosomatids their feces. During study period number patients suspected acute...

We characterized the bacterial diversity of newly produced steelmaking wastes (NPSW) and deposited (SWD) in a restricted land area, generated by siderurgic industry, using 16S rDNA clone library approach. A total 212 partial-length sequences were analyzed, revealing 123 distinct operational taxonomic units (OTUs) determined DOTUR program to 97% sequence similarity. Phylogenetic analysis from NPSW SWD libraries demonstrated that Gammaproteobacteria, Betaproteobacteria, Alphaproteobacteria,...

Do Ceará, estado com problema de Doença Chagas, Russas tem recebido a maior soma investigações, verificando-se 11,6% infecção humana, em 1959. Recentemente foram examinados cães, gatos e outros animais encontrando-se altas taxas infecção. Selecionou-se, na presente pesquisa, uma micro-área (Fazenda Boa Vista) região, onde haviam sido antes 52 cães 21 gatos, 11 10 positivos. Foram mais 12 1 5 positivos, respectivamente, elevando-se taxa positividade global para 29,5%. Nessa 125 pessoas pela...

The protozoan parasite Trypanosoma cruzi causes the Chagas disease, which final outcome can be morbidity or death. complexity of this infection is due to many kinds players involved in immune response and variety host cells targeted by parasite. We built an ordinary differential equation model includes aspects innate adaptive study T. infection. also cardiomyocytes represent how affects heart. used parasitemia experimental data infected wild-type mice estimate parameters. investigated number...

ABSTRACT Here, we report the draft genome sequence of Staphylococcus aureus strain LC33, isolated from human breast milk in Brazil. This microorganism has been typed as ST1/t127/sccmecV. To our knowledge, this is first a methicillin-resistant S. milk.

Infection by Leishmania can cause diseases ranging from self-healing cutaneous to visceral dissemination that lead death if untreated. In order explore the early phase of infection and role macrophages, we implement a system differential equations involving major players in innate immune response leishmaniasis (i.e., parasites intracellular free form, infected uninfected NO/ROS). The model was adjusted validated using data C57BL/6, KO SCID mice published literature. key findings were...

spp. commonly infects phagocytic cells of the immune system, particularly macrophages, employing various evasion strategies that enable their survival by altering intracellular environment. In mammals, these parasites establish persistent infections modulating gene expression in thus interfering with signaling and response pathways, ultimately creating a favorable environment for parasite's reproduction. this study, our objective was to use data mining subsequent filtering techniques...

Abstract Background Although the pancreatic ductal adenocarcinoma (PDAC) presents high mortality and metastatic potential, there is a lack of effective therapies low survival rate for this disease. This PDAC scenario urges new strategies diagnosis, drug targets, treatment. Methods We performed gene expression microarray meta-analysis tumor against healthy tissues in order to identify differentially expressed genes shared among all datasets, named core-genes (CG). confirmed proteins CG...

Dynamic operation is one of the current challenges in optical communication and networks, adaptive control amplifier (ACOP) problems this challenge. The ACOP approaches aim to define gains amplifiers dynamically increase quality transmission after a cascade amplifiers. most recent approach uses multiobjective evolutionary optimization algorithm maximize signal noise ratio (OSNR) minimize OSNR ripple. Despite promising results regarding Quality Transmission, it not desirable rely on an make...

ABSTRACT We report here the draft genome sequences of two community-associated methicillin-resistant Staphylococcus aureus (CA-MRSA) strains, C18 and C80, isolated from healthy children day care centers. To our knowledge, these are first CA-MRSA ST398/CC398/Scc mec V ST5/CC5/Scc IVa in Brazil.

The Adaptive Control of optical amplifier Operating Point (ACOP) is one the problems presented in challenge Dynamic operation communication and networks. ACOP approaches aim to define gains amplifiers dynamically increase transmission quality after a cascade amplifiers. most recent approach uses multi-objective evolutionary optimization algorithm maximize Optical Signal Noise Ratio (OSNR) minimize OSNR ripple. Despite promising results regarding Quality Transmission, relying on an make...