A5018143168- Trypanosoma species research and implications
- Research on Leishmaniasis Studies
- Adipokines, Inflammation, and Metabolic Diseases
- Immune Cell Function and Interaction
- Regulation of Appetite and Obesity
- Tuberculosis Research and Epidemiology
- Insect symbiosis and bacterial influences
- Helminth infection and control
- Biochemical and Molecular Research
- Immune responses and vaccinations
- Immune cells in cancer
- Mosquito-borne diseases and control
- Parasite Biology and Host Interactions
- Apelin-related biomedical research
- RNA regulation and disease
- Insect Resistance and Genetics
- Adipose Tissue and Metabolism
- Neuroinflammation and Neurodegeneration Mechanisms
- Parasites and Host Interactions
- Endoplasmic Reticulum Stress and Disease
- Dietary Effects on Health
- CAR-T cell therapy research
- CRISPR and Genetic Engineering
- Galectins and Cancer Biology
- Viral Infectious Diseases and Gene Expression in Insects
Universidade Federal de Santa Catarina
2018-2025
Universidade Estadual de Campinas (UNICAMP)
2022-2025
Universidade Federal de Minas Gerais
2011-2024
Institute of Parasitology
2020
Tissue-resident macrophages are the most abundant immune cell population in healthy adipose tissue. Adipose tissue (ATMs) change during metabolic stress and thought to contribute syndrome. Here, we studied ATM subpopulations steady state response nutritional infectious challenges. We found that tissue-resident from epididymal white (eWAT) tightly associate with blood vessels, displaying very high endocytic capacity. refer these cells as vasculature-associated ATMs (VAMs). Chronic high-fat...
The protozoan parasite Trypanosoma cruzi is the etiologic agent of Chagas disease, a highly debilitating human pathology that affects millions people in Americas. sequencing this parasite's genome reveals trans-sialidase/trans-sialidase-like (TcS), polymorphic protein family known to be involved several aspects T. biology, largest gene family, encoding more than 1,400 genes. Despite fact four TcS groups are well characterized and only one contains active trans-sialidases, all members...
Trypanosoma cruzi, the etiologic agent of Chagas disease, is currently divided into six discrete typing units (DTUs), named TcI–TcVI. CL Brener, reference strain T. cruzi genome project, a hybrid with assembled 41 putative chromosomes. Gene copy number variation (CNV) well documented as an important mechanism to enhance gene expression and variability in cruzi. Chromosomal CNV (CCNV) another level which whole blocks genes are expanded simultaneously. Although karyotype not defined, several...
Background Trypanosoma rangeli is a hemoflagellate protozoan parasite infecting humans and other wild domestic mammals across Central South America. It does not cause human disease, but it can be mistaken for the etiologic agent of Chagas cruzi. We have sequenced T. genome to provide new tools elucidating distinct intriguing biology this species key pathways related interaction with its arthropod mammalian hosts. Methodology/Principal Findings The haploid ∼24 Mb in length, smallest least...
Endosymbiont-bearing trypanosomatids have been considered excellent models for the study of cell evolution because host protozoan co-evolves with an intracellular bacterium in a mutualistic relationship. Such protozoa inhabit single invertebrate during their entire life cycle and exhibit special characteristics that group them particular phylogenetic cluster Trypanosomatidae family, thus classified as monoxenics. In effort to better understand such symbiotic association, we used DNA...
Background Trypanosoma cruzi is the etiological agent of Chagas disease, a debilitating illness that affects millions people in Americas. A major finding T. genome project was discovery novel multigene family composed approximately 1,300 genes encode mucin-associated surface proteins (MASPs). The high level polymorphism MASP associated with its localization at infective forms parasite suggests participates host–parasite interactions. We speculate large repertoire sequences may contribute to...
Trypanosoma cruzi, the protozoan that causes Chagas disease, has a complex life cycle involving several morphologically and biochemically distinct stages establish intricate interactions with various insect mammalian hosts. It also heterogeneous population structure comprising strains properties such as virulence, sensitivity to drugs, antigenic profile tissue tropism. We present comparative transcriptome analysis of two cloned T. cruzi display contrasting virulence phenotypes in animal...
The Leishmania (Viannia) braziliensis complex is responsible for most cases of New World tegumentary leishmaniasis. This includes two closely related species but with different geographic distribution and disease phenotypes, L. (V.) peruviana braziliensis. However, the genetic basis these differences not well understood status as distinct has been questioned by some. Here we sequenced genomes isolates (LEM1537 PAB-4377) using Illumina high throughput sequencing performed comparative analyses...
Trypanosoma cruzi, the etiologic agent of Chagas disease, is currently divided into six discrete typing units (DTUs), named TcI-TcVI. TcII among major DTUs enrolled in human infections South America southern cone, where it associated with severe cardiac and digestive symptoms. Despite importance disease epidemiology pathology, so far, no genome-wide comparisons mitochondrial nuclear genomes field isolates have been performed to track variability evolution this DTU endemic regions.In present...
Background The factors influencing variation in the clinical forms of Chagas disease have not been elucidated; however, it is likely that genetics both host and parasite are involved. Several studies attempted to correlate T. cruzi strains involved infection with by using hemoculture and/or PCR-based genotyping parasites from infected human tissues. However, techniques limitations hamper analysis large numbers samples. goal this work was identify conserved polymorphic linear B-cell epitopes...
Proteins containing repetitive amino acid domains are widespread in all life forms. In parasitic organisms, proteins repeats play important roles such as cell adhesion and invasion immune evasion. Therefore, extracellular intracellular parasites expected to be under different selective pressures regarding the content their genomes. Here, we investigated whether there is a bias found predicted proteomes of 6 exclusively 17 obligate protozoan parasites, well 4 free-living protists. We also...
Monocyte counts are increased during human tuberculosis (TB) but it has not been determined whether Mycobacterium (Mtb) directly regulates myeloid commitment. We demonstrated that exposure to Mtb directs primary CD34+ cells differentiate into monocytes/macrophages. In vitro conversion did require type I or II IFN signaling. contrast, enhanced IL-6 responses by cell cultures and IL-6R neutralization inhibited differentiation decreased mycobacterial growth in vitro. Integrated systems biology...
Hematopoiesis changes to adapt the physiology of development and aging. Temporal in hematopoiesis parallel age-dependent incidences blood diseases. Several heterochronic regulators have been identified, but how master transcription factor (TF) circuitry definitive hematopoietic stem cells (HSCs) adapts over lifespan is unknown. Here, we show that expression ETS family TF Erg adult-biased, programmed upregulation during juvenile adult aging evolutionarily conserved required for complete...
ABSTRACT Despite the early recognition that individuals living with obesity are more prone to develop adverse outcomes during COVID‐19, mechanisms underlying these conditions still unclear. During obesity, an accumulation of free fatty acids (FFAs) in circulation promotes low‐grade inflammation. Here, we show FFAs induce epigenetic reprogramming monocytes, exacerbating their inflammatory profile after SARS‐CoV‐2 infection, a mechanism named metabolic‐primed immunity. Monocytes from people or...
One of the fundamental features that make viruses intracellular parasites is necessity to use cellular translational machinery. Hence, this a crucial checkpoint for controlling infections. Here, we show dengue and Zika viruses, responsible nearly 400 million infections every year worldwide, explore such control optimal replication. Using immunocompetent cells, demonstrate arrest protein translations happens after sensing dsRNA information required avoid blocking contained in viral 5'-UTR....
Microgliosis plays a critical role in diet-induced hypothalamic inflammation. A few hours after high-fat diet (HFD), microglia shift to an inflammatory phenotype, and prolonged fat consumption leads the recruitment of bone marrow-derived cells hypothalamus. However, transcriptional signatures functions these remain unclear. Using dual-reporter mice, this study reveals that CX3CR1-positive exhibit minimal changes response HFD, while significant differences emerge between CCR2-positive...
Sequences that have several copies in a genome, such as multicopy-gene families, mobile elements, and microsatellites, are among the most challenging genomic segments to study. They frequently underestimated genome assemblies, hampering correct assessment of these important players evolution adaptation.
While diseases caused by nematodes remains a considerable drawback for the livestock, agriculture and public health, anthelmintics drug resistance has been observed over past years is major concern parasite control. Ivermectin, initially considered as highly potent drug, currently presents reduced anti-helminthic efficacy, which influenced expression of several ATP-binding cassette transporters (ABC), among them P-glycoproteins (Pgps). Here we present some evidences Pgps dominance during...
Trematodes have complex life cycles with multiple hosts. Biomphalaria snails commonly act as the first intermediate hosts of several species that can affect human and animal health. The specific identification larval trematodes found in is difficult limited, since taxonomy these flukes based on morphological traits adults vertebrates. Despite recent advances worldwide, studies aiming at use molecular tools for cercariae are scarce South America. In fact, most focused Schistosoma mansoni, few...
Genomic and epidemiological surveillance are paramount for the discovery of new viruses with potential to cross species barriers. Here, we present a member genus Alphavirus found in Trichoprosopon Wyeomia mosquitoes, tentatively named Pirahy virus (PIRAV). PIRAV was isolated from mosquito pools collected rural area Piraí do Sul, South Brazil. In vitro assays revealed that replicates causes cytopathic effects vertebrate cell lines such as Vero E6, SH-SY5Y, BHK-21 UMNSAH/DF-1. signature...
Molecular genetic markers are one of the most informative and widely used genome features in clinical environmental diagnostic studies. A polymerase chain reaction (PCR)-based molecular marker is very attractive because it suitable to high throughput automation confers specificity. However, design taxon-specific primers may be difficult time consuming due need identify appropriate genomic regions for annealing evaluate primer specificity.Here, we report development a Tool Identification...
Abstract Monocyte counts are increased during human tuberculosis (TB) but it has not been determined whether Mycobacterium ( Mtb ) directly regulates myeloid commitment. We demonstrated that exposure to directs primary CD34 + cells differentiate into monocytes/macrophages. In vitro conversion did require type I or II IFN signaling. contrast, enhanced IL-6 responses by cell cultures and IL-6R neutralization inhibited differentiation decreased mycobacterial growth in vitro. Integrated systems...
Abstract Microgliosis is an important component of diet-induced hypothalamic inflammation in obesity. A few hours after the introduction a high-fat diet, mediobasal hypothalamus resident microglia undergo morphological and functional changes toward inflammatory phenotype. If consumption large amounts dietary fats persists for long periods, bone marrow- derived myeloid cells are recruited integrated into new landscape microglia. However, it currently unknown what transcriptional signatures...